Cargando…
Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana
Mass drug administration (MDA) is the current mainstay to interrupt the transmission of lymphatic filariasis. To monitor whether MDA is effective and transmission of lymphatic filariasis indeed has been interrupted, rigorous surveillance is required. Assessment of transmission by programme managers...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6473662/ https://www.ncbi.nlm.nih.gov/pubmed/30884886 http://dx.doi.org/10.3390/tropicalmed4010049 |
_version_ | 1783412480626655232 |
---|---|
author | Pi-Bansa, Sellase Osei, Joseph H. N. Kartey-Attipoe, Worlasi D. Elhassan, Elizabeth Agyemang, David Otoo, Sampson Dadzie, Samuel K. Appawu, Maxwell A. Wilson, Michael D. Koudou, Benjamin G. de Souza, Dziedzom K. Utzinger, Jürg Boakye, Daniel A. |
author_facet | Pi-Bansa, Sellase Osei, Joseph H. N. Kartey-Attipoe, Worlasi D. Elhassan, Elizabeth Agyemang, David Otoo, Sampson Dadzie, Samuel K. Appawu, Maxwell A. Wilson, Michael D. Koudou, Benjamin G. de Souza, Dziedzom K. Utzinger, Jürg Boakye, Daniel A. |
author_sort | Pi-Bansa, Sellase |
collection | PubMed |
description | Mass drug administration (MDA) is the current mainstay to interrupt the transmission of lymphatic filariasis. To monitor whether MDA is effective and transmission of lymphatic filariasis indeed has been interrupted, rigorous surveillance is required. Assessment of transmission by programme managers is usually done via serology. New research suggests that xenomonitoring holds promise for determining the success of lymphatic filariasis interventions. The objective of this study was to assess Wuchereria bancrofti infection in mosquitoes as a post-MDA surveillance tool using xenomonitoring. The study was carried out in four districts of Ghana; Ahanta West, Mpohor, Kassena Nankana West and Bongo. A suite of mosquito sampling methods was employed, including human landing collections, pyrethrum spray catches and window exit traps. Infection of W. bancrofti in mosquitoes was determined using dissection, conventional and real-time polymerase chain reaction and loop mediated isothermal amplification assays. Aedes, Anopheles coustani, An. gambiae, An. pharoensis, Culex and Mansonia mosquitoes were sampled in each of the four study districts. The dissected mosquitoes were positive for filarial infection using molecular assays. Dissected An. melas mosquitoes from Ahanta West district were the only species found positive for filarial parasites. We conclude that whilst samples extracted with Trizol reagent did not show any positives, molecular methods should still be considered for monitoring and surveillance of lymphatic filariasis transmission. |
format | Online Article Text |
id | pubmed-6473662 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64736622019-04-29 Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana Pi-Bansa, Sellase Osei, Joseph H. N. Kartey-Attipoe, Worlasi D. Elhassan, Elizabeth Agyemang, David Otoo, Sampson Dadzie, Samuel K. Appawu, Maxwell A. Wilson, Michael D. Koudou, Benjamin G. de Souza, Dziedzom K. Utzinger, Jürg Boakye, Daniel A. Trop Med Infect Dis Article Mass drug administration (MDA) is the current mainstay to interrupt the transmission of lymphatic filariasis. To monitor whether MDA is effective and transmission of lymphatic filariasis indeed has been interrupted, rigorous surveillance is required. Assessment of transmission by programme managers is usually done via serology. New research suggests that xenomonitoring holds promise for determining the success of lymphatic filariasis interventions. The objective of this study was to assess Wuchereria bancrofti infection in mosquitoes as a post-MDA surveillance tool using xenomonitoring. The study was carried out in four districts of Ghana; Ahanta West, Mpohor, Kassena Nankana West and Bongo. A suite of mosquito sampling methods was employed, including human landing collections, pyrethrum spray catches and window exit traps. Infection of W. bancrofti in mosquitoes was determined using dissection, conventional and real-time polymerase chain reaction and loop mediated isothermal amplification assays. Aedes, Anopheles coustani, An. gambiae, An. pharoensis, Culex and Mansonia mosquitoes were sampled in each of the four study districts. The dissected mosquitoes were positive for filarial infection using molecular assays. Dissected An. melas mosquitoes from Ahanta West district were the only species found positive for filarial parasites. We conclude that whilst samples extracted with Trizol reagent did not show any positives, molecular methods should still be considered for monitoring and surveillance of lymphatic filariasis transmission. MDPI 2019-03-17 /pmc/articles/PMC6473662/ /pubmed/30884886 http://dx.doi.org/10.3390/tropicalmed4010049 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pi-Bansa, Sellase Osei, Joseph H. N. Kartey-Attipoe, Worlasi D. Elhassan, Elizabeth Agyemang, David Otoo, Sampson Dadzie, Samuel K. Appawu, Maxwell A. Wilson, Michael D. Koudou, Benjamin G. de Souza, Dziedzom K. Utzinger, Jürg Boakye, Daniel A. Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana |
title | Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana |
title_full | Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana |
title_fullStr | Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana |
title_full_unstemmed | Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana |
title_short | Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana |
title_sort | assessing the presence of wuchereria bancrofti infections in vectors using xenomonitoring in lymphatic filariasis endemic districts in ghana |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6473662/ https://www.ncbi.nlm.nih.gov/pubmed/30884886 http://dx.doi.org/10.3390/tropicalmed4010049 |
work_keys_str_mv | AT pibansasellase assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana AT oseijosephhn assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana AT karteyattipoeworlasid assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana AT elhassanelizabeth assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana AT agyemangdavid assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana AT otoosampson assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana AT dadziesamuelk assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana AT appawumaxwella assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana AT wilsonmichaeld assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana AT koudoubenjaming assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana AT desouzadziedzomk assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana AT utzingerjurg assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana AT boakyedaniela assessingthepresenceofwuchereriabancroftiinfectionsinvectorsusingxenomonitoringinlymphaticfilariasisendemicdistrictsinghana |