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Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana

Mass drug administration (MDA) is the current mainstay to interrupt the transmission of lymphatic filariasis. To monitor whether MDA is effective and transmission of lymphatic filariasis indeed has been interrupted, rigorous surveillance is required. Assessment of transmission by programme managers...

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Autores principales: Pi-Bansa, Sellase, Osei, Joseph H. N., Kartey-Attipoe, Worlasi D., Elhassan, Elizabeth, Agyemang, David, Otoo, Sampson, Dadzie, Samuel K., Appawu, Maxwell A., Wilson, Michael D., Koudou, Benjamin G., de Souza, Dziedzom K., Utzinger, Jürg, Boakye, Daniel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6473662/
https://www.ncbi.nlm.nih.gov/pubmed/30884886
http://dx.doi.org/10.3390/tropicalmed4010049
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author Pi-Bansa, Sellase
Osei, Joseph H. N.
Kartey-Attipoe, Worlasi D.
Elhassan, Elizabeth
Agyemang, David
Otoo, Sampson
Dadzie, Samuel K.
Appawu, Maxwell A.
Wilson, Michael D.
Koudou, Benjamin G.
de Souza, Dziedzom K.
Utzinger, Jürg
Boakye, Daniel A.
author_facet Pi-Bansa, Sellase
Osei, Joseph H. N.
Kartey-Attipoe, Worlasi D.
Elhassan, Elizabeth
Agyemang, David
Otoo, Sampson
Dadzie, Samuel K.
Appawu, Maxwell A.
Wilson, Michael D.
Koudou, Benjamin G.
de Souza, Dziedzom K.
Utzinger, Jürg
Boakye, Daniel A.
author_sort Pi-Bansa, Sellase
collection PubMed
description Mass drug administration (MDA) is the current mainstay to interrupt the transmission of lymphatic filariasis. To monitor whether MDA is effective and transmission of lymphatic filariasis indeed has been interrupted, rigorous surveillance is required. Assessment of transmission by programme managers is usually done via serology. New research suggests that xenomonitoring holds promise for determining the success of lymphatic filariasis interventions. The objective of this study was to assess Wuchereria bancrofti infection in mosquitoes as a post-MDA surveillance tool using xenomonitoring. The study was carried out in four districts of Ghana; Ahanta West, Mpohor, Kassena Nankana West and Bongo. A suite of mosquito sampling methods was employed, including human landing collections, pyrethrum spray catches and window exit traps. Infection of W. bancrofti in mosquitoes was determined using dissection, conventional and real-time polymerase chain reaction and loop mediated isothermal amplification assays. Aedes, Anopheles coustani, An. gambiae, An. pharoensis, Culex and Mansonia mosquitoes were sampled in each of the four study districts. The dissected mosquitoes were positive for filarial infection using molecular assays. Dissected An. melas mosquitoes from Ahanta West district were the only species found positive for filarial parasites. We conclude that whilst samples extracted with Trizol reagent did not show any positives, molecular methods should still be considered for monitoring and surveillance of lymphatic filariasis transmission.
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spelling pubmed-64736622019-04-29 Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana Pi-Bansa, Sellase Osei, Joseph H. N. Kartey-Attipoe, Worlasi D. Elhassan, Elizabeth Agyemang, David Otoo, Sampson Dadzie, Samuel K. Appawu, Maxwell A. Wilson, Michael D. Koudou, Benjamin G. de Souza, Dziedzom K. Utzinger, Jürg Boakye, Daniel A. Trop Med Infect Dis Article Mass drug administration (MDA) is the current mainstay to interrupt the transmission of lymphatic filariasis. To monitor whether MDA is effective and transmission of lymphatic filariasis indeed has been interrupted, rigorous surveillance is required. Assessment of transmission by programme managers is usually done via serology. New research suggests that xenomonitoring holds promise for determining the success of lymphatic filariasis interventions. The objective of this study was to assess Wuchereria bancrofti infection in mosquitoes as a post-MDA surveillance tool using xenomonitoring. The study was carried out in four districts of Ghana; Ahanta West, Mpohor, Kassena Nankana West and Bongo. A suite of mosquito sampling methods was employed, including human landing collections, pyrethrum spray catches and window exit traps. Infection of W. bancrofti in mosquitoes was determined using dissection, conventional and real-time polymerase chain reaction and loop mediated isothermal amplification assays. Aedes, Anopheles coustani, An. gambiae, An. pharoensis, Culex and Mansonia mosquitoes were sampled in each of the four study districts. The dissected mosquitoes were positive for filarial infection using molecular assays. Dissected An. melas mosquitoes from Ahanta West district were the only species found positive for filarial parasites. We conclude that whilst samples extracted with Trizol reagent did not show any positives, molecular methods should still be considered for monitoring and surveillance of lymphatic filariasis transmission. MDPI 2019-03-17 /pmc/articles/PMC6473662/ /pubmed/30884886 http://dx.doi.org/10.3390/tropicalmed4010049 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pi-Bansa, Sellase
Osei, Joseph H. N.
Kartey-Attipoe, Worlasi D.
Elhassan, Elizabeth
Agyemang, David
Otoo, Sampson
Dadzie, Samuel K.
Appawu, Maxwell A.
Wilson, Michael D.
Koudou, Benjamin G.
de Souza, Dziedzom K.
Utzinger, Jürg
Boakye, Daniel A.
Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana
title Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana
title_full Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana
title_fullStr Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana
title_full_unstemmed Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana
title_short Assessing the Presence of Wuchereria bancrofti Infections in Vectors Using Xenomonitoring in Lymphatic Filariasis Endemic Districts in Ghana
title_sort assessing the presence of wuchereria bancrofti infections in vectors using xenomonitoring in lymphatic filariasis endemic districts in ghana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6473662/
https://www.ncbi.nlm.nih.gov/pubmed/30884886
http://dx.doi.org/10.3390/tropicalmed4010049
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