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Development of a Portable Dielectric Biosensor for Rapid Detection of Viscosity Variations and Its In Vitro Evaluations Using Saliva Samples of COPD Patients and Healthy Control
Chronic Obstructive Pulmonary Disease (COPD) is a life-threatening lung disease affecting millions of people worldwide. Although the majority of patients with objective COPD go undiagnosed until the late stages of their disease, recent studies suggest that the regular screening of sputum viscosity c...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6473814/ https://www.ncbi.nlm.nih.gov/pubmed/30654570 http://dx.doi.org/10.3390/healthcare7010011 |
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author | Soltani Zarrin, Pouya Ibne Jamal, Farabi Roeckendorf, Niels Wenger, Christian |
author_facet | Soltani Zarrin, Pouya Ibne Jamal, Farabi Roeckendorf, Niels Wenger, Christian |
author_sort | Soltani Zarrin, Pouya |
collection | PubMed |
description | Chronic Obstructive Pulmonary Disease (COPD) is a life-threatening lung disease affecting millions of people worldwide. Although the majority of patients with objective COPD go undiagnosed until the late stages of their disease, recent studies suggest that the regular screening of sputum viscosity could provide important information on the disease detection. Since the viscosity of sputum is mainly defined by its mucin–protein and water contents, dielectric biosensors can be used for detection of viscosity variations by screening changes in sputum’s contents. Therefore, the objective of this work was to develop a portable dielectric biosensor for rapid detection of viscosity changes and to evaluate its clinical performance in characterizing viscosity differences of saliva samples collected from COPD patients and Healthy Control (HC). For this purpose, a portable dielectric biosensor, capable of providing real-time measurements, was developed. The sensor performance for dielectric characterization of mediums with high water content, such as saliva, was evaluated using isopropanol–water mixtures. Subsequently, saliva samples, collected from COPD patients and HC, were investigated for clinical assessments. The radio frequency biosensor provided high repeatability of 1.1% throughout experiments. High repeatability, ease of cleaning, low-cost, and portability of the biosensor made it a suitable technology for point-of-care applications. |
format | Online Article Text |
id | pubmed-6473814 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64738142019-05-02 Development of a Portable Dielectric Biosensor for Rapid Detection of Viscosity Variations and Its In Vitro Evaluations Using Saliva Samples of COPD Patients and Healthy Control Soltani Zarrin, Pouya Ibne Jamal, Farabi Roeckendorf, Niels Wenger, Christian Healthcare (Basel) Article Chronic Obstructive Pulmonary Disease (COPD) is a life-threatening lung disease affecting millions of people worldwide. Although the majority of patients with objective COPD go undiagnosed until the late stages of their disease, recent studies suggest that the regular screening of sputum viscosity could provide important information on the disease detection. Since the viscosity of sputum is mainly defined by its mucin–protein and water contents, dielectric biosensors can be used for detection of viscosity variations by screening changes in sputum’s contents. Therefore, the objective of this work was to develop a portable dielectric biosensor for rapid detection of viscosity changes and to evaluate its clinical performance in characterizing viscosity differences of saliva samples collected from COPD patients and Healthy Control (HC). For this purpose, a portable dielectric biosensor, capable of providing real-time measurements, was developed. The sensor performance for dielectric characterization of mediums with high water content, such as saliva, was evaluated using isopropanol–water mixtures. Subsequently, saliva samples, collected from COPD patients and HC, were investigated for clinical assessments. The radio frequency biosensor provided high repeatability of 1.1% throughout experiments. High repeatability, ease of cleaning, low-cost, and portability of the biosensor made it a suitable technology for point-of-care applications. MDPI 2019-01-16 /pmc/articles/PMC6473814/ /pubmed/30654570 http://dx.doi.org/10.3390/healthcare7010011 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Soltani Zarrin, Pouya Ibne Jamal, Farabi Roeckendorf, Niels Wenger, Christian Development of a Portable Dielectric Biosensor for Rapid Detection of Viscosity Variations and Its In Vitro Evaluations Using Saliva Samples of COPD Patients and Healthy Control |
title | Development of a Portable Dielectric Biosensor for Rapid Detection of Viscosity Variations and Its In Vitro Evaluations Using Saliva Samples of COPD Patients and Healthy Control |
title_full | Development of a Portable Dielectric Biosensor for Rapid Detection of Viscosity Variations and Its In Vitro Evaluations Using Saliva Samples of COPD Patients and Healthy Control |
title_fullStr | Development of a Portable Dielectric Biosensor for Rapid Detection of Viscosity Variations and Its In Vitro Evaluations Using Saliva Samples of COPD Patients and Healthy Control |
title_full_unstemmed | Development of a Portable Dielectric Biosensor for Rapid Detection of Viscosity Variations and Its In Vitro Evaluations Using Saliva Samples of COPD Patients and Healthy Control |
title_short | Development of a Portable Dielectric Biosensor for Rapid Detection of Viscosity Variations and Its In Vitro Evaluations Using Saliva Samples of COPD Patients and Healthy Control |
title_sort | development of a portable dielectric biosensor for rapid detection of viscosity variations and its in vitro evaluations using saliva samples of copd patients and healthy control |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6473814/ https://www.ncbi.nlm.nih.gov/pubmed/30654570 http://dx.doi.org/10.3390/healthcare7010011 |
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