A Cautionary Tale: Endogenous Biotinylated Proteins and Exogenously-Introduced Protein A Cause Antibody-Independent Artefacts in Western Blot Studies of Brain-Derived Proteins

Antibodies are commonly used to detect or isolate proteins from biological samples. Much attention has been paid to the potential for poorly-characterized antibodies to lead to misleading results, but antibody-independent artefacts may also occur. Here, we recount two examples of antibody-independen...

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Detalles Bibliográficos
Autores principales: Grant, Marianne K. O., Shapiro, Samantha L., Ashe, Karen H., Liu, Peng, Zahs, Kathleen R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474067/
https://www.ncbi.nlm.nih.gov/pubmed/31019379
http://dx.doi.org/10.1186/s12575-019-0095-z

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