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Maspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma

AIM: Barrett’s esophagus (BE) is a predisposing factor of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma (ECA/GEJ Aca). BE patients are stratified and subsequently monitored according to the risk of malignant progression by the combination of endoscopy and biopsy. This study is t...

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Autores principales: Dzinic, Sijana H., Mahdi, Zaid, Bernardo, M. Margarida, Vranic, Semir, Beydoun, Haya, Nahra, Nadine, Alijagic, Amra, Harajli, Deanna, Pang, Aaron, Saliganan, Dan M., Rahman, Abid M., Skenderi, Faruk, Hasanbegovic, Berisa, Dyson, Gregory, Beydoun, Rafic, Sheng, Shijie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474598/
https://www.ncbi.nlm.nih.gov/pubmed/31002675
http://dx.doi.org/10.1371/journal.pone.0215089
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author Dzinic, Sijana H.
Mahdi, Zaid
Bernardo, M. Margarida
Vranic, Semir
Beydoun, Haya
Nahra, Nadine
Alijagic, Amra
Harajli, Deanna
Pang, Aaron
Saliganan, Dan M.
Rahman, Abid M.
Skenderi, Faruk
Hasanbegovic, Berisa
Dyson, Gregory
Beydoun, Rafic
Sheng, Shijie
author_facet Dzinic, Sijana H.
Mahdi, Zaid
Bernardo, M. Margarida
Vranic, Semir
Beydoun, Haya
Nahra, Nadine
Alijagic, Amra
Harajli, Deanna
Pang, Aaron
Saliganan, Dan M.
Rahman, Abid M.
Skenderi, Faruk
Hasanbegovic, Berisa
Dyson, Gregory
Beydoun, Rafic
Sheng, Shijie
author_sort Dzinic, Sijana H.
collection PubMed
description AIM: Barrett’s esophagus (BE) is a predisposing factor of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma (ECA/GEJ Aca). BE patients are stratified and subsequently monitored according to the risk of malignant progression by the combination of endoscopy and biopsy. This study is to evaluate the maspin expression patterns as early diagnostic markers of malignancy in BE patients. MATERIALS AND METHODS: Immunohistochemistry (IHC) staining was performed on 62 archival core biopsies from 35 patients, including BE without dysplasia (intestinal metaplasia, IM), BE with low grade dysplasia, BE with high grade dysplasia, carcinoma in situ, and well to poorly differentiated ECA/GEJ Aca (PD-ECA/GEJ Aca). The intensity and the subcellular distribution of immunoreactivity were evaluated microscopically. Statistical analysis was performed using the χ(2) and Fisher exact tests. RESULTS: The level of epithelial-specific tumor suppressor maspin protein inversely correlated with the progression from IM to PD-ECA/GEJ Aca. Lesions of each pathological grade could be divided into subtypes that exhibited distinct maspin subcellular distribution patterns, including nuclear only (Nuc), combined nuclear and cytoplasmic (Nuc+Cyt), cytoplasmic only (Cyt) and overall negligible (Neg). The Cyt subtype, which was minor in both IM and dysplasia (approximately 10%), was predominant in ECA/GEJ Aca as early as well-differentiated lesions (more than 50%: p = 0.0092). In comparison, nuclear staining of the tumor suppressor TP53 was heterogeneous in dysplasia, and did not correlate with the differentiation grades of ECA/GEJ Aca. CONCLUSION: The Cyt subtype of maspin expression pattern in core biopsies of BE patients may serve as a molecular marker for early diagnosis of ECA/GEJ Aca.
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spelling pubmed-64745982019-05-03 Maspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma Dzinic, Sijana H. Mahdi, Zaid Bernardo, M. Margarida Vranic, Semir Beydoun, Haya Nahra, Nadine Alijagic, Amra Harajli, Deanna Pang, Aaron Saliganan, Dan M. Rahman, Abid M. Skenderi, Faruk Hasanbegovic, Berisa Dyson, Gregory Beydoun, Rafic Sheng, Shijie PLoS One Research Article AIM: Barrett’s esophagus (BE) is a predisposing factor of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma (ECA/GEJ Aca). BE patients are stratified and subsequently monitored according to the risk of malignant progression by the combination of endoscopy and biopsy. This study is to evaluate the maspin expression patterns as early diagnostic markers of malignancy in BE patients. MATERIALS AND METHODS: Immunohistochemistry (IHC) staining was performed on 62 archival core biopsies from 35 patients, including BE without dysplasia (intestinal metaplasia, IM), BE with low grade dysplasia, BE with high grade dysplasia, carcinoma in situ, and well to poorly differentiated ECA/GEJ Aca (PD-ECA/GEJ Aca). The intensity and the subcellular distribution of immunoreactivity were evaluated microscopically. Statistical analysis was performed using the χ(2) and Fisher exact tests. RESULTS: The level of epithelial-specific tumor suppressor maspin protein inversely correlated with the progression from IM to PD-ECA/GEJ Aca. Lesions of each pathological grade could be divided into subtypes that exhibited distinct maspin subcellular distribution patterns, including nuclear only (Nuc), combined nuclear and cytoplasmic (Nuc+Cyt), cytoplasmic only (Cyt) and overall negligible (Neg). The Cyt subtype, which was minor in both IM and dysplasia (approximately 10%), was predominant in ECA/GEJ Aca as early as well-differentiated lesions (more than 50%: p = 0.0092). In comparison, nuclear staining of the tumor suppressor TP53 was heterogeneous in dysplasia, and did not correlate with the differentiation grades of ECA/GEJ Aca. CONCLUSION: The Cyt subtype of maspin expression pattern in core biopsies of BE patients may serve as a molecular marker for early diagnosis of ECA/GEJ Aca. Public Library of Science 2019-04-19 /pmc/articles/PMC6474598/ /pubmed/31002675 http://dx.doi.org/10.1371/journal.pone.0215089 Text en © 2019 Dzinic et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dzinic, Sijana H.
Mahdi, Zaid
Bernardo, M. Margarida
Vranic, Semir
Beydoun, Haya
Nahra, Nadine
Alijagic, Amra
Harajli, Deanna
Pang, Aaron
Saliganan, Dan M.
Rahman, Abid M.
Skenderi, Faruk
Hasanbegovic, Berisa
Dyson, Gregory
Beydoun, Rafic
Sheng, Shijie
Maspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma
title Maspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma
title_full Maspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma
title_fullStr Maspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma
title_full_unstemmed Maspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma
title_short Maspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma
title_sort maspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474598/
https://www.ncbi.nlm.nih.gov/pubmed/31002675
http://dx.doi.org/10.1371/journal.pone.0215089
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