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Fluid supplementation accelerates epithelial repair during chemical colitis
The dextran sulfate sodium (DSS) model of colitis is a common animal model of inflammatory bowel disease that causes pain and distress. In this study, we aimed to determine whether fluid supplementation can be used as a welfare-based intervention to minimize animal suffering. C57Bl/6 females undergo...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474653/ https://www.ncbi.nlm.nih.gov/pubmed/31002683 http://dx.doi.org/10.1371/journal.pone.0215387 |
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author | Burgueño, Juan F. Lang, Jessica K. Santander, Ana M. Fernández, Irina Fernández, Ester Zaias, Julia Abreu, Maria T. |
author_facet | Burgueño, Juan F. Lang, Jessica K. Santander, Ana M. Fernández, Irina Fernández, Ester Zaias, Julia Abreu, Maria T. |
author_sort | Burgueño, Juan F. |
collection | PubMed |
description | The dextran sulfate sodium (DSS) model of colitis is a common animal model of inflammatory bowel disease that causes pain and distress. In this study, we aimed to determine whether fluid supplementation can be used as a welfare-based intervention to minimize animal suffering. C57Bl/6 females undergoing acute colitis by administration of 3% DSS in drinking water were supplemented with 1 mL intraperitoneal injections of NaCl and compared to non-supplemented control mice. Mouse behavior and locomotive activity were assessed on days 5–6 after DSS initiation by means of tail suspension, novel object recognition and open field activity tests. Mice were euthanized after either the acute (day 7) or the recovery phase (day 12) of colitis and inflammation, epithelial proliferation, and differentiation were assessed by means of histology, immunohistochemistry, quantitative PCR, and western blot. We found that fluid-supplemented mice had reduced signs of colitis with no alterations in behavior or locomotive activity. Furthermore, we observed an accelerated epithelial repair response after fluid hydration during the acute phase of colitis, characterized by increased crypt proliferation, activation of ERK1/2, and modulation of TGF-β1 expression. Consistent with these findings, fluid-supplemented mice had increased numbers of goblet cells, upregulated expression of differentiation markers for absorptive enterocytes, and reduced inflammation during the recovery phase. Our results show that fluid hydration does not reduce stress in DSS-treated mice but alters colitis evolution by reducing clinical signs and accelerating epithelial repair. These results argue against the routine use of fluid supplementation in DSS-treated mice. |
format | Online Article Text |
id | pubmed-6474653 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64746532019-05-03 Fluid supplementation accelerates epithelial repair during chemical colitis Burgueño, Juan F. Lang, Jessica K. Santander, Ana M. Fernández, Irina Fernández, Ester Zaias, Julia Abreu, Maria T. PLoS One Research Article The dextran sulfate sodium (DSS) model of colitis is a common animal model of inflammatory bowel disease that causes pain and distress. In this study, we aimed to determine whether fluid supplementation can be used as a welfare-based intervention to minimize animal suffering. C57Bl/6 females undergoing acute colitis by administration of 3% DSS in drinking water were supplemented with 1 mL intraperitoneal injections of NaCl and compared to non-supplemented control mice. Mouse behavior and locomotive activity were assessed on days 5–6 after DSS initiation by means of tail suspension, novel object recognition and open field activity tests. Mice were euthanized after either the acute (day 7) or the recovery phase (day 12) of colitis and inflammation, epithelial proliferation, and differentiation were assessed by means of histology, immunohistochemistry, quantitative PCR, and western blot. We found that fluid-supplemented mice had reduced signs of colitis with no alterations in behavior or locomotive activity. Furthermore, we observed an accelerated epithelial repair response after fluid hydration during the acute phase of colitis, characterized by increased crypt proliferation, activation of ERK1/2, and modulation of TGF-β1 expression. Consistent with these findings, fluid-supplemented mice had increased numbers of goblet cells, upregulated expression of differentiation markers for absorptive enterocytes, and reduced inflammation during the recovery phase. Our results show that fluid hydration does not reduce stress in DSS-treated mice but alters colitis evolution by reducing clinical signs and accelerating epithelial repair. These results argue against the routine use of fluid supplementation in DSS-treated mice. Public Library of Science 2019-04-19 /pmc/articles/PMC6474653/ /pubmed/31002683 http://dx.doi.org/10.1371/journal.pone.0215387 Text en © 2019 Burgueño et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Burgueño, Juan F. Lang, Jessica K. Santander, Ana M. Fernández, Irina Fernández, Ester Zaias, Julia Abreu, Maria T. Fluid supplementation accelerates epithelial repair during chemical colitis |
title | Fluid supplementation accelerates epithelial repair during chemical colitis |
title_full | Fluid supplementation accelerates epithelial repair during chemical colitis |
title_fullStr | Fluid supplementation accelerates epithelial repair during chemical colitis |
title_full_unstemmed | Fluid supplementation accelerates epithelial repair during chemical colitis |
title_short | Fluid supplementation accelerates epithelial repair during chemical colitis |
title_sort | fluid supplementation accelerates epithelial repair during chemical colitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474653/ https://www.ncbi.nlm.nih.gov/pubmed/31002683 http://dx.doi.org/10.1371/journal.pone.0215387 |
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