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Chrysanthemum extract attenuates hepatotoxicity via inhibiting oxidative stress in vivo and in vitro
BACKGROUND: ‘Bianliang ziyu’, a famous chrysanthemum variety commonly planted in Kaifeng, China, is often consumed by local residents. However, the hepatoprotective effects of Bianliang ziyu and their underlying mechanisms are not clear. OBJECTIVE: In this study, we investigated the hepatoprotective...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Open Academia
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475127/ https://www.ncbi.nlm.nih.gov/pubmed/31024225 http://dx.doi.org/10.29219/fnr.v63.1667 |
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author | Tian, Zixia Jia, Haiyan Jin, Yuezhen Wang, Minghui Kou, Jiejian Wang, Chunli Rong, Xuli Xie, Xinmei Han, Guang Pang, Xiaobin |
author_facet | Tian, Zixia Jia, Haiyan Jin, Yuezhen Wang, Minghui Kou, Jiejian Wang, Chunli Rong, Xuli Xie, Xinmei Han, Guang Pang, Xiaobin |
author_sort | Tian, Zixia |
collection | PubMed |
description | BACKGROUND: ‘Bianliang ziyu’, a famous chrysanthemum variety commonly planted in Kaifeng, China, is often consumed by local residents. However, the hepatoprotective effects of Bianliang ziyu and their underlying mechanisms are not clear. OBJECTIVE: In this study, we investigated the hepatoprotective and antioxidative effects of Bianliang ziyu extract (BZE) on liver injury and explored its molecular mechanisms. DESIGN: Sprague-Dawley rats were administered BZE by intragastric administration for 8–9 days, and then alcohol or carbon tetrachloride (CCl(4)) was administered by gavage to induce acute liver injury. The activities of serum alanine aminotransferase, aspartate aminotransferase, superoxide dismutase, and malondialdehyde in the rats were measured, and the liver of each rat was examined for histopathological changes. In vitro, HL-7702 cells were pretreated with BZE for 24 h and then exposed to 30 mmol•L(−1) acetaminophen (APAP) for 12 h. The survival rate of the cells and the alanine aminotransferase and aspartate aminotransferase activities were determined. Then, we investigated the effects of BZE on oxidative stress, apoptosis, and the activation of nuclear factor erythroid-2-related factor 2 (Nrf2) signaling in HL-7702 cells induced by APAP. RESULTS: The results showed that BZE prevented alcohol-, CCl(4)-, and APAP-induced liver injury and suppressed hepatic oxidative stress in vitro and in vivo. BZE was also observed to significantly inhibit the reduction of mitochondrial membrane potential and regulate the expression of Bcl-2, Bax and Caspase-3 in APAP-induced HL-7702 cells. In addition, BZE significantly promoted nuclear translocation and the expression of Nrf2 as well as its downstream gene hemeoxygenase-1 (HO-1) in vitro. Furthermore, the findings showed that Nrf2 siRNA reversed the effects of BZE on cell survival and apoptosis-related protein expression in APAP-induced HL-7702 cells. CONCLUSIONS: BZE plays an important role in preventing hepatotoxicity by inhibiting oxidative stress and apoptosis through activation of Nrf2 signaling. BZE could be developed as an effective functional food for protecting the liver. |
format | Online Article Text |
id | pubmed-6475127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Open Academia |
record_format | MEDLINE/PubMed |
spelling | pubmed-64751272019-04-25 Chrysanthemum extract attenuates hepatotoxicity via inhibiting oxidative stress in vivo and in vitro Tian, Zixia Jia, Haiyan Jin, Yuezhen Wang, Minghui Kou, Jiejian Wang, Chunli Rong, Xuli Xie, Xinmei Han, Guang Pang, Xiaobin Food Nutr Res Original Article BACKGROUND: ‘Bianliang ziyu’, a famous chrysanthemum variety commonly planted in Kaifeng, China, is often consumed by local residents. However, the hepatoprotective effects of Bianliang ziyu and their underlying mechanisms are not clear. OBJECTIVE: In this study, we investigated the hepatoprotective and antioxidative effects of Bianliang ziyu extract (BZE) on liver injury and explored its molecular mechanisms. DESIGN: Sprague-Dawley rats were administered BZE by intragastric administration for 8–9 days, and then alcohol or carbon tetrachloride (CCl(4)) was administered by gavage to induce acute liver injury. The activities of serum alanine aminotransferase, aspartate aminotransferase, superoxide dismutase, and malondialdehyde in the rats were measured, and the liver of each rat was examined for histopathological changes. In vitro, HL-7702 cells were pretreated with BZE for 24 h and then exposed to 30 mmol•L(−1) acetaminophen (APAP) for 12 h. The survival rate of the cells and the alanine aminotransferase and aspartate aminotransferase activities were determined. Then, we investigated the effects of BZE on oxidative stress, apoptosis, and the activation of nuclear factor erythroid-2-related factor 2 (Nrf2) signaling in HL-7702 cells induced by APAP. RESULTS: The results showed that BZE prevented alcohol-, CCl(4)-, and APAP-induced liver injury and suppressed hepatic oxidative stress in vitro and in vivo. BZE was also observed to significantly inhibit the reduction of mitochondrial membrane potential and regulate the expression of Bcl-2, Bax and Caspase-3 in APAP-induced HL-7702 cells. In addition, BZE significantly promoted nuclear translocation and the expression of Nrf2 as well as its downstream gene hemeoxygenase-1 (HO-1) in vitro. Furthermore, the findings showed that Nrf2 siRNA reversed the effects of BZE on cell survival and apoptosis-related protein expression in APAP-induced HL-7702 cells. CONCLUSIONS: BZE plays an important role in preventing hepatotoxicity by inhibiting oxidative stress and apoptosis through activation of Nrf2 signaling. BZE could be developed as an effective functional food for protecting the liver. Open Academia 2019-04-15 /pmc/articles/PMC6475127/ /pubmed/31024225 http://dx.doi.org/10.29219/fnr.v63.1667 Text en © 2019 Zixia Tian et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license. |
spellingShingle | Original Article Tian, Zixia Jia, Haiyan Jin, Yuezhen Wang, Minghui Kou, Jiejian Wang, Chunli Rong, Xuli Xie, Xinmei Han, Guang Pang, Xiaobin Chrysanthemum extract attenuates hepatotoxicity via inhibiting oxidative stress in vivo and in vitro |
title | Chrysanthemum extract attenuates hepatotoxicity via inhibiting oxidative stress in vivo and in vitro |
title_full | Chrysanthemum extract attenuates hepatotoxicity via inhibiting oxidative stress in vivo and in vitro |
title_fullStr | Chrysanthemum extract attenuates hepatotoxicity via inhibiting oxidative stress in vivo and in vitro |
title_full_unstemmed | Chrysanthemum extract attenuates hepatotoxicity via inhibiting oxidative stress in vivo and in vitro |
title_short | Chrysanthemum extract attenuates hepatotoxicity via inhibiting oxidative stress in vivo and in vitro |
title_sort | chrysanthemum extract attenuates hepatotoxicity via inhibiting oxidative stress in vivo and in vitro |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475127/ https://www.ncbi.nlm.nih.gov/pubmed/31024225 http://dx.doi.org/10.29219/fnr.v63.1667 |
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