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Changes in Gastric Smooth Muscle Cell Contraction during Pregnancy: Effect of Estrogen

It is well known that pregnancy is associated with frequent gastrointestinal (GI) disorders and symptoms. Moreover, previous reports have shown that estrogen, which changes in levels during pregnancy, participates in the regulation of GI motility and is involved in the pathogenesis of various functi...

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Autores principales: Al-Shboul, Othman A., Al-Rshoud, Hanan J., Al-Dwairi, Ahmed N., Alqudah, Mohammad A., Alfaqih, Mahmoud A., Mustafa, Ayman G., Jaafar, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475543/
https://www.ncbi.nlm.nih.gov/pubmed/31080672
http://dx.doi.org/10.1155/2019/4302309
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author Al-Shboul, Othman A.
Al-Rshoud, Hanan J.
Al-Dwairi, Ahmed N.
Alqudah, Mohammad A.
Alfaqih, Mahmoud A.
Mustafa, Ayman G.
Jaafar, Mohammad
author_facet Al-Shboul, Othman A.
Al-Rshoud, Hanan J.
Al-Dwairi, Ahmed N.
Alqudah, Mohammad A.
Alfaqih, Mahmoud A.
Mustafa, Ayman G.
Jaafar, Mohammad
author_sort Al-Shboul, Othman A.
collection PubMed
description It is well known that pregnancy is associated with frequent gastrointestinal (GI) disorders and symptoms. Moreover, previous reports have shown that estrogen, which changes in levels during pregnancy, participates in the regulation of GI motility and is involved in the pathogenesis of various functional disorders in the stomach. The aim of the current study was to explore the changes in the expression of estrogen receptors (ERs) and examine the effect of estrogen on nitric oxide- (NO-) cyclic guanosine monophosphate (cGMP) pathway and thus relaxation in gastric smooth muscle cells (GSMC) during pregnancy. Single GSMC from early-pregnant and late-pregnant Sprague-Dawley rats were used. Protein and mRNA expression levels of ERs were measured via specifically designed enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), respectively. NO and cGMP levels were measured via specifically designed ELISA kits. Effect of estrogen on acetylcholine- (ACh-) induced contraction of single GSMC was measured via scanning micrometry in the presence or absence of the NO synthase inhibitor, N-nitro-L-arginine (L-NNA), or guanylyl cyclase inhibitor, 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ). Estrogen increased both NO and cGMP levels and their levels were greater in early compared to late pregnancy. Expression of ERs was greater in early compared to late pregnancy. ACh induced greater contraction of GSMC in late pregnancy compared to early pregnancy. Estrogen inhibited ACh-induced contraction in both periods of pregnancy. Importantly, pretreatment of GSMC with either L-NNA or ODQ abolished estrogen inhibitory action on muscle contraction. In conclusion, GSMC contractile behavior undergoes drastic changes in response to estrogen during pregnancy and this might explain some of the pregnancy-associated gastric disorders.
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spelling pubmed-64755432019-05-12 Changes in Gastric Smooth Muscle Cell Contraction during Pregnancy: Effect of Estrogen Al-Shboul, Othman A. Al-Rshoud, Hanan J. Al-Dwairi, Ahmed N. Alqudah, Mohammad A. Alfaqih, Mahmoud A. Mustafa, Ayman G. Jaafar, Mohammad J Pregnancy Research Article It is well known that pregnancy is associated with frequent gastrointestinal (GI) disorders and symptoms. Moreover, previous reports have shown that estrogen, which changes in levels during pregnancy, participates in the regulation of GI motility and is involved in the pathogenesis of various functional disorders in the stomach. The aim of the current study was to explore the changes in the expression of estrogen receptors (ERs) and examine the effect of estrogen on nitric oxide- (NO-) cyclic guanosine monophosphate (cGMP) pathway and thus relaxation in gastric smooth muscle cells (GSMC) during pregnancy. Single GSMC from early-pregnant and late-pregnant Sprague-Dawley rats were used. Protein and mRNA expression levels of ERs were measured via specifically designed enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), respectively. NO and cGMP levels were measured via specifically designed ELISA kits. Effect of estrogen on acetylcholine- (ACh-) induced contraction of single GSMC was measured via scanning micrometry in the presence or absence of the NO synthase inhibitor, N-nitro-L-arginine (L-NNA), or guanylyl cyclase inhibitor, 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ). Estrogen increased both NO and cGMP levels and their levels were greater in early compared to late pregnancy. Expression of ERs was greater in early compared to late pregnancy. ACh induced greater contraction of GSMC in late pregnancy compared to early pregnancy. Estrogen inhibited ACh-induced contraction in both periods of pregnancy. Importantly, pretreatment of GSMC with either L-NNA or ODQ abolished estrogen inhibitory action on muscle contraction. In conclusion, GSMC contractile behavior undergoes drastic changes in response to estrogen during pregnancy and this might explain some of the pregnancy-associated gastric disorders. Hindawi 2019-04-04 /pmc/articles/PMC6475543/ /pubmed/31080672 http://dx.doi.org/10.1155/2019/4302309 Text en Copyright © 2019 Othman A. Al-Shboul et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Al-Shboul, Othman A.
Al-Rshoud, Hanan J.
Al-Dwairi, Ahmed N.
Alqudah, Mohammad A.
Alfaqih, Mahmoud A.
Mustafa, Ayman G.
Jaafar, Mohammad
Changes in Gastric Smooth Muscle Cell Contraction during Pregnancy: Effect of Estrogen
title Changes in Gastric Smooth Muscle Cell Contraction during Pregnancy: Effect of Estrogen
title_full Changes in Gastric Smooth Muscle Cell Contraction during Pregnancy: Effect of Estrogen
title_fullStr Changes in Gastric Smooth Muscle Cell Contraction during Pregnancy: Effect of Estrogen
title_full_unstemmed Changes in Gastric Smooth Muscle Cell Contraction during Pregnancy: Effect of Estrogen
title_short Changes in Gastric Smooth Muscle Cell Contraction during Pregnancy: Effect of Estrogen
title_sort changes in gastric smooth muscle cell contraction during pregnancy: effect of estrogen
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475543/
https://www.ncbi.nlm.nih.gov/pubmed/31080672
http://dx.doi.org/10.1155/2019/4302309
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