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Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder
Chicken egg yolk is an easily available source for the isolation of sialylglycopeptides (SGP) carrying homogenous biantennary N-glycans. This approach has gained much attention in the last decade since these SGPs can easily be used for the semi-synthesis of glycoconjugates circumventing laborious fu...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475658/ https://www.ncbi.nlm.nih.gov/pubmed/31016140 http://dx.doi.org/10.1016/j.mex.2019.04.007 |
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author | Alagesan, Kathirvel Kolarich, Daniel |
author_facet | Alagesan, Kathirvel Kolarich, Daniel |
author_sort | Alagesan, Kathirvel |
collection | PubMed |
description | Chicken egg yolk is an easily available source for the isolation of sialylglycopeptides (SGP) carrying homogenous biantennary N-glycans. This approach has gained much attention in the last decade since these SGPs can easily be used for the semi-synthesis of glycoconjugates circumventing laborious full-synthetic methodologies. Here we report an optimised, significantly shorter (one day instead of five) and environmentally friendly procedure for the mg scale isolation of SGP using commercially available egg yolk powder. A single chromatographic step following chloroform/methanol precipitation of proteins and lipids yielded desired approximately 200 mg SGP from 250 g egg yolk powder within a day. • Environmentally friendly procedure for isolation of sialylglycopeptide from Egg yolk powder. • Reduced the protocol from five days down to one. |
format | Online Article Text |
id | pubmed-6475658 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-64756582019-04-23 Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder Alagesan, Kathirvel Kolarich, Daniel MethodsX Agricultural and Biological Science Chicken egg yolk is an easily available source for the isolation of sialylglycopeptides (SGP) carrying homogenous biantennary N-glycans. This approach has gained much attention in the last decade since these SGPs can easily be used for the semi-synthesis of glycoconjugates circumventing laborious full-synthetic methodologies. Here we report an optimised, significantly shorter (one day instead of five) and environmentally friendly procedure for the mg scale isolation of SGP using commercially available egg yolk powder. A single chromatographic step following chloroform/methanol precipitation of proteins and lipids yielded desired approximately 200 mg SGP from 250 g egg yolk powder within a day. • Environmentally friendly procedure for isolation of sialylglycopeptide from Egg yolk powder. • Reduced the protocol from five days down to one. Elsevier 2019-04-09 /pmc/articles/PMC6475658/ /pubmed/31016140 http://dx.doi.org/10.1016/j.mex.2019.04.007 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Agricultural and Biological Science Alagesan, Kathirvel Kolarich, Daniel Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder |
title | Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder |
title_full | Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder |
title_fullStr | Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder |
title_full_unstemmed | Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder |
title_short | Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder |
title_sort | improved strategy for large scale isolation of sialylglycopeptide (sgp) from egg yolk powder |
topic | Agricultural and Biological Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475658/ https://www.ncbi.nlm.nih.gov/pubmed/31016140 http://dx.doi.org/10.1016/j.mex.2019.04.007 |
work_keys_str_mv | AT alagesankathirvel improvedstrategyforlargescaleisolationofsialylglycopeptidesgpfromeggyolkpowder AT kolarichdaniel improvedstrategyforlargescaleisolationofsialylglycopeptidesgpfromeggyolkpowder |