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Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells

MicroRNAs (miRNAs) have been widely demonstrated to interact with multiple cellular signaling pathways and to participate in a wide range of physiological processes. Estradiol-17β (E2) is the most potent and prevalent endogenous estrogen that plays a vital role in promoting bone formation and reduci...

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Autores principales: Liu, Guanqi, Lu, Yeming, Mai, Zhihui, Liu, Runheng, Peng, Zhuli, Chen, Lin, Chen, Zheng, Wang, Ruizhi, Ai, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476012/
https://www.ncbi.nlm.nih.gov/pubmed/31089333
http://dx.doi.org/10.1155/2019/7547506
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author Liu, Guanqi
Lu, Yeming
Mai, Zhihui
Liu, Runheng
Peng, Zhuli
Chen, Lin
Chen, Zheng
Wang, Ruizhi
Ai, Hong
author_facet Liu, Guanqi
Lu, Yeming
Mai, Zhihui
Liu, Runheng
Peng, Zhuli
Chen, Lin
Chen, Zheng
Wang, Ruizhi
Ai, Hong
author_sort Liu, Guanqi
collection PubMed
description MicroRNAs (miRNAs) have been widely demonstrated to interact with multiple cellular signaling pathways and to participate in a wide range of physiological processes. Estradiol-17β (E2) is the most potent and prevalent endogenous estrogen that plays a vital role in promoting bone formation and reducing bone resorption. Currently, little is known about the regulation of miRNAs in E2-induced osteogenic differentiation. In the present study, the primary bone marrow mesenchymal stem cells from rats (rBMSCs) were isolated and incubated with E2, followed by miRNA profiling. The microarray showed that 29 miRNAs were differentially expressed in response to E2 stimulation. Further verification by real-time reverse-transcriptase polymerase chain reaction revealed that E2 enhanced the expression of let-7b and miR-25 but suppressed the miR-30b expression. Moreover, a gain-of-function experiment confirmed that miR-30b negatively regulated the E2-induced osteogenic differentiation. These data suggest an important role of miRNAs in osteogenic differentiation.
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spelling pubmed-64760122019-05-14 Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells Liu, Guanqi Lu, Yeming Mai, Zhihui Liu, Runheng Peng, Zhuli Chen, Lin Chen, Zheng Wang, Ruizhi Ai, Hong Stem Cells Int Research Article MicroRNAs (miRNAs) have been widely demonstrated to interact with multiple cellular signaling pathways and to participate in a wide range of physiological processes. Estradiol-17β (E2) is the most potent and prevalent endogenous estrogen that plays a vital role in promoting bone formation and reducing bone resorption. Currently, little is known about the regulation of miRNAs in E2-induced osteogenic differentiation. In the present study, the primary bone marrow mesenchymal stem cells from rats (rBMSCs) were isolated and incubated with E2, followed by miRNA profiling. The microarray showed that 29 miRNAs were differentially expressed in response to E2 stimulation. Further verification by real-time reverse-transcriptase polymerase chain reaction revealed that E2 enhanced the expression of let-7b and miR-25 but suppressed the miR-30b expression. Moreover, a gain-of-function experiment confirmed that miR-30b negatively regulated the E2-induced osteogenic differentiation. These data suggest an important role of miRNAs in osteogenic differentiation. Hindawi 2019-04-04 /pmc/articles/PMC6476012/ /pubmed/31089333 http://dx.doi.org/10.1155/2019/7547506 Text en Copyright © 2019 Guanqi Liu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Guanqi
Lu, Yeming
Mai, Zhihui
Liu, Runheng
Peng, Zhuli
Chen, Lin
Chen, Zheng
Wang, Ruizhi
Ai, Hong
Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells
title Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells
title_full Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells
title_fullStr Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells
title_full_unstemmed Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells
title_short Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells
title_sort suppressing microrna-30b by estrogen promotes osteogenesis in bone marrow mesenchymal stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476012/
https://www.ncbi.nlm.nih.gov/pubmed/31089333
http://dx.doi.org/10.1155/2019/7547506
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