Cargando…
Phenotypic variation of cassava root traits and their responses to drought
PREMISE OF THE STUDY: The key to increased cassava production is balancing the trade‐off between marketable roots and traits that drive nutrient and water uptake. However, only a small number of protocols have been developed for cassava roots. Here, we introduce a set of new variables and methods to...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476172/ https://www.ncbi.nlm.nih.gov/pubmed/31024782 http://dx.doi.org/10.1002/aps3.1238 |
_version_ | 1783412862284201984 |
---|---|
author | Kengkanna, Jitrana Jakaew, Phissinee Amawan, Suwaluk Busener, Natalie Bucksch, Alexander Saengwilai, Patompong |
author_facet | Kengkanna, Jitrana Jakaew, Phissinee Amawan, Suwaluk Busener, Natalie Bucksch, Alexander Saengwilai, Patompong |
author_sort | Kengkanna, Jitrana |
collection | PubMed |
description | PREMISE OF THE STUDY: The key to increased cassava production is balancing the trade‐off between marketable roots and traits that drive nutrient and water uptake. However, only a small number of protocols have been developed for cassava roots. Here, we introduce a set of new variables and methods to phenotype cassava roots and enhance breeding pipelines. METHODS: Different cassava genotypes were planted in pot and field conditions under well‐watered and drought treatments. We developed cassava shovelomics and used digital imaging of root traits (DIRT) to evaluate geometrical root traits in addition to common traits (e.g., length, number). RESULTS: Cassava shovelomics and DIRT were successfully implemented to extract root phenotypes, and a large phenotypic variation for root traits was observed. Significant correlations were found among root traits measured manually and by DIRT. Drought significantly decreased shoot dry weight, total root number, and root length by 84%, 30%, and 25%, respectively. High adventitious root number was associated with increased shoot dry weight (r = 0.44) under drought. DISCUSSION: Our methods allow for high‐throughput cassava root phenotyping, which makes a breeding program targeting root traits feasible. We suggest that root number is a breeding target for improved cassava production under drought. |
format | Online Article Text |
id | pubmed-6476172 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64761722019-04-25 Phenotypic variation of cassava root traits and their responses to drought Kengkanna, Jitrana Jakaew, Phissinee Amawan, Suwaluk Busener, Natalie Bucksch, Alexander Saengwilai, Patompong Appl Plant Sci Application Articles PREMISE OF THE STUDY: The key to increased cassava production is balancing the trade‐off between marketable roots and traits that drive nutrient and water uptake. However, only a small number of protocols have been developed for cassava roots. Here, we introduce a set of new variables and methods to phenotype cassava roots and enhance breeding pipelines. METHODS: Different cassava genotypes were planted in pot and field conditions under well‐watered and drought treatments. We developed cassava shovelomics and used digital imaging of root traits (DIRT) to evaluate geometrical root traits in addition to common traits (e.g., length, number). RESULTS: Cassava shovelomics and DIRT were successfully implemented to extract root phenotypes, and a large phenotypic variation for root traits was observed. Significant correlations were found among root traits measured manually and by DIRT. Drought significantly decreased shoot dry weight, total root number, and root length by 84%, 30%, and 25%, respectively. High adventitious root number was associated with increased shoot dry weight (r = 0.44) under drought. DISCUSSION: Our methods allow for high‐throughput cassava root phenotyping, which makes a breeding program targeting root traits feasible. We suggest that root number is a breeding target for improved cassava production under drought. John Wiley and Sons Inc. 2019-04-10 /pmc/articles/PMC6476172/ /pubmed/31024782 http://dx.doi.org/10.1002/aps3.1238 Text en © 2019 Kengkanna et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Application Articles Kengkanna, Jitrana Jakaew, Phissinee Amawan, Suwaluk Busener, Natalie Bucksch, Alexander Saengwilai, Patompong Phenotypic variation of cassava root traits and their responses to drought |
title | Phenotypic variation of cassava root traits and their responses to drought |
title_full | Phenotypic variation of cassava root traits and their responses to drought |
title_fullStr | Phenotypic variation of cassava root traits and their responses to drought |
title_full_unstemmed | Phenotypic variation of cassava root traits and their responses to drought |
title_short | Phenotypic variation of cassava root traits and their responses to drought |
title_sort | phenotypic variation of cassava root traits and their responses to drought |
topic | Application Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476172/ https://www.ncbi.nlm.nih.gov/pubmed/31024782 http://dx.doi.org/10.1002/aps3.1238 |
work_keys_str_mv | AT kengkannajitrana phenotypicvariationofcassavaroottraitsandtheirresponsestodrought AT jakaewphissinee phenotypicvariationofcassavaroottraitsandtheirresponsestodrought AT amawansuwaluk phenotypicvariationofcassavaroottraitsandtheirresponsestodrought AT busenernatalie phenotypicvariationofcassavaroottraitsandtheirresponsestodrought AT buckschalexander phenotypicvariationofcassavaroottraitsandtheirresponsestodrought AT saengwilaipatompong phenotypicvariationofcassavaroottraitsandtheirresponsestodrought |