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Circulation of the Cultivable Symbiont Serratia symbiotica in Aphids Is Mediated by Plants
Symbiosis is a common phenomenon in nature that substantially affects organismal ecology and evolution. Fundamental questions regarding how mutualistic associations arise and evolve in nature remain, however, poorly studied. The aphid-Serratia symbiotica bacterium interaction represents a valuable m...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476230/ https://www.ncbi.nlm.nih.gov/pubmed/31037067 http://dx.doi.org/10.3389/fmicb.2019.00764 |
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author | Pons, Inès Renoz, François Noël, Christine Hance, Thierry |
author_facet | Pons, Inès Renoz, François Noël, Christine Hance, Thierry |
author_sort | Pons, Inès |
collection | PubMed |
description | Symbiosis is a common phenomenon in nature that substantially affects organismal ecology and evolution. Fundamental questions regarding how mutualistic associations arise and evolve in nature remain, however, poorly studied. The aphid-Serratia symbiotica bacterium interaction represents a valuable model to study mechanisms shaping these symbiotic interspecific interactions. S. symbiotica strains capable of living independently of aphid hosts have recently been isolated. These strains probably resulted from horizontal transfers and could be an evolutionary link to an intra-organismal symbiosis. In this context, we used the tripartite interaction between the aphid Aphis fabae, a cultivable S. symbiotica bacterium, and the host plant Vicia faba to evaluate the bacterium ability to circulate in this system, exploring its environmental acquisition by aphids and horizontal transmission between aphids via the host plant. Using molecular analyses and fluorescence techniques, we showed that the cultivable S. symbiotica can enter the plants and induce new bacterial infections in aphids feeding on these new infected plants. Remarkably, we also found that the bacterium can have positive effects on the host plant, mainly at the root level. Furthermore, our results demonstrated that cultivable S. symbiotica can be horizontally transferred from infected to uninfected aphids sharing the same plant, providing first direct evidence that plants can mediate horizontal transmission of certain strains of this symbiont species. These findings highlight the importance of considering symbiotic associations in complex systems where microorganisms can circulate between different compartments. Our study can thus have major implications for understanding the multifaceted interactions between microbes, insects and plants. |
format | Online Article Text |
id | pubmed-6476230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64762302019-04-29 Circulation of the Cultivable Symbiont Serratia symbiotica in Aphids Is Mediated by Plants Pons, Inès Renoz, François Noël, Christine Hance, Thierry Front Microbiol Microbiology Symbiosis is a common phenomenon in nature that substantially affects organismal ecology and evolution. Fundamental questions regarding how mutualistic associations arise and evolve in nature remain, however, poorly studied. The aphid-Serratia symbiotica bacterium interaction represents a valuable model to study mechanisms shaping these symbiotic interspecific interactions. S. symbiotica strains capable of living independently of aphid hosts have recently been isolated. These strains probably resulted from horizontal transfers and could be an evolutionary link to an intra-organismal symbiosis. In this context, we used the tripartite interaction between the aphid Aphis fabae, a cultivable S. symbiotica bacterium, and the host plant Vicia faba to evaluate the bacterium ability to circulate in this system, exploring its environmental acquisition by aphids and horizontal transmission between aphids via the host plant. Using molecular analyses and fluorescence techniques, we showed that the cultivable S. symbiotica can enter the plants and induce new bacterial infections in aphids feeding on these new infected plants. Remarkably, we also found that the bacterium can have positive effects on the host plant, mainly at the root level. Furthermore, our results demonstrated that cultivable S. symbiotica can be horizontally transferred from infected to uninfected aphids sharing the same plant, providing first direct evidence that plants can mediate horizontal transmission of certain strains of this symbiont species. These findings highlight the importance of considering symbiotic associations in complex systems where microorganisms can circulate between different compartments. Our study can thus have major implications for understanding the multifaceted interactions between microbes, insects and plants. Frontiers Media S.A. 2019-04-15 /pmc/articles/PMC6476230/ /pubmed/31037067 http://dx.doi.org/10.3389/fmicb.2019.00764 Text en Copyright © 2019 Pons, Renoz, Noël and Hance. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Pons, Inès Renoz, François Noël, Christine Hance, Thierry Circulation of the Cultivable Symbiont Serratia symbiotica in Aphids Is Mediated by Plants |
title | Circulation of the Cultivable Symbiont Serratia symbiotica in Aphids Is Mediated by Plants |
title_full | Circulation of the Cultivable Symbiont Serratia symbiotica in Aphids Is Mediated by Plants |
title_fullStr | Circulation of the Cultivable Symbiont Serratia symbiotica in Aphids Is Mediated by Plants |
title_full_unstemmed | Circulation of the Cultivable Symbiont Serratia symbiotica in Aphids Is Mediated by Plants |
title_short | Circulation of the Cultivable Symbiont Serratia symbiotica in Aphids Is Mediated by Plants |
title_sort | circulation of the cultivable symbiont serratia symbiotica in aphids is mediated by plants |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476230/ https://www.ncbi.nlm.nih.gov/pubmed/31037067 http://dx.doi.org/10.3389/fmicb.2019.00764 |
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