Cargando…

Next‐generation phylogeography of the cockle Cerastoderma glaucum: Highly heterogeneous genetic differentiation in a lagoon species

AIM: Coastal lagoons form an intriguing example of fragmented marine habitats. Restricted gene flow among isolated populations of lagoon species may promote their genetic divergence and may thus provide a first step toward speciation. In the present study, the population genetic structure of the lag...

Descripción completa

Detalles Bibliográficos
Autores principales: Sromek, Ludmila, Forcioli, Didier, Lasota, Rafal, Furla, Paola, Wolowicz, Maciej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476780/
https://www.ncbi.nlm.nih.gov/pubmed/31031934
http://dx.doi.org/10.1002/ece3.5070
_version_ 1783412929416134656
author Sromek, Ludmila
Forcioli, Didier
Lasota, Rafal
Furla, Paola
Wolowicz, Maciej
author_facet Sromek, Ludmila
Forcioli, Didier
Lasota, Rafal
Furla, Paola
Wolowicz, Maciej
author_sort Sromek, Ludmila
collection PubMed
description AIM: Coastal lagoons form an intriguing example of fragmented marine habitats. Restricted gene flow among isolated populations of lagoon species may promote their genetic divergence and may thus provide a first step toward speciation. In the present study, the population genetic structure of the lagoon cockle Cerastoderma glaucum has been investigated to clarify the complex phylogeographic pattern found in previous studies, to localize major genetic breaks, and to discuss their origin and maintenance. LOCATION: The Atlantic and Mediterranean coasts, including the Baltic, North Sea, and Black Sea. METHODS: A total of 204 C. glaucum individuals from 14 populations were genotyped using restriction site‐associated DNA sequencing (RADseq). The genetic diversity, divergence, and structure were analyzed using genome‐wide single nucleotide polymorphisms (SNPs). Phylogenetic relationships were inferred under a coalescent model using svdquartets. RESULTS: The RADseq approach allowed inferring phylogeographic relationships with an unprecedented resolution. Three deeply divergent lineages were identified within C. glaucum that are separated by many genetic barriers: one lineage in the Aegean–Black Sea region, one in the Ionian Sea, and the last one widely distributed from the Western Mediterranean to the Baltic Sea. The nested branching pattern displayed on the species tree largely agrees with the likely scenario of C. glaucum postglacial expansion from the Mediterranean to the Baltic Sea. MAIN CONCLUSION: The genetic differentiations between geographically separated lagoons proved to be strong, highlighting the evolutionary influence of these naturally fragmented habitats. The postglacial expansion created complex patterns of spatial segregation of genetic diversity with allele frequency gradients in many outlier loci, but also discrepancies between the nuclear and mitochondrial genetic markers that probably arose from genetic surfing of mitochondrial variation.
format Online
Article
Text
id pubmed-6476780
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-64767802019-04-26 Next‐generation phylogeography of the cockle Cerastoderma glaucum: Highly heterogeneous genetic differentiation in a lagoon species Sromek, Ludmila Forcioli, Didier Lasota, Rafal Furla, Paola Wolowicz, Maciej Ecol Evol Original Research AIM: Coastal lagoons form an intriguing example of fragmented marine habitats. Restricted gene flow among isolated populations of lagoon species may promote their genetic divergence and may thus provide a first step toward speciation. In the present study, the population genetic structure of the lagoon cockle Cerastoderma glaucum has been investigated to clarify the complex phylogeographic pattern found in previous studies, to localize major genetic breaks, and to discuss their origin and maintenance. LOCATION: The Atlantic and Mediterranean coasts, including the Baltic, North Sea, and Black Sea. METHODS: A total of 204 C. glaucum individuals from 14 populations were genotyped using restriction site‐associated DNA sequencing (RADseq). The genetic diversity, divergence, and structure were analyzed using genome‐wide single nucleotide polymorphisms (SNPs). Phylogenetic relationships were inferred under a coalescent model using svdquartets. RESULTS: The RADseq approach allowed inferring phylogeographic relationships with an unprecedented resolution. Three deeply divergent lineages were identified within C. glaucum that are separated by many genetic barriers: one lineage in the Aegean–Black Sea region, one in the Ionian Sea, and the last one widely distributed from the Western Mediterranean to the Baltic Sea. The nested branching pattern displayed on the species tree largely agrees with the likely scenario of C. glaucum postglacial expansion from the Mediterranean to the Baltic Sea. MAIN CONCLUSION: The genetic differentiations between geographically separated lagoons proved to be strong, highlighting the evolutionary influence of these naturally fragmented habitats. The postglacial expansion created complex patterns of spatial segregation of genetic diversity with allele frequency gradients in many outlier loci, but also discrepancies between the nuclear and mitochondrial genetic markers that probably arose from genetic surfing of mitochondrial variation. John Wiley and Sons Inc. 2019-03-27 /pmc/articles/PMC6476780/ /pubmed/31031934 http://dx.doi.org/10.1002/ece3.5070 Text en © 2019 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Sromek, Ludmila
Forcioli, Didier
Lasota, Rafal
Furla, Paola
Wolowicz, Maciej
Next‐generation phylogeography of the cockle Cerastoderma glaucum: Highly heterogeneous genetic differentiation in a lagoon species
title Next‐generation phylogeography of the cockle Cerastoderma glaucum: Highly heterogeneous genetic differentiation in a lagoon species
title_full Next‐generation phylogeography of the cockle Cerastoderma glaucum: Highly heterogeneous genetic differentiation in a lagoon species
title_fullStr Next‐generation phylogeography of the cockle Cerastoderma glaucum: Highly heterogeneous genetic differentiation in a lagoon species
title_full_unstemmed Next‐generation phylogeography of the cockle Cerastoderma glaucum: Highly heterogeneous genetic differentiation in a lagoon species
title_short Next‐generation phylogeography of the cockle Cerastoderma glaucum: Highly heterogeneous genetic differentiation in a lagoon species
title_sort next‐generation phylogeography of the cockle cerastoderma glaucum: highly heterogeneous genetic differentiation in a lagoon species
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476780/
https://www.ncbi.nlm.nih.gov/pubmed/31031934
http://dx.doi.org/10.1002/ece3.5070
work_keys_str_mv AT sromekludmila nextgenerationphylogeographyofthecocklecerastodermaglaucumhighlyheterogeneousgeneticdifferentiationinalagoonspecies
AT forciolididier nextgenerationphylogeographyofthecocklecerastodermaglaucumhighlyheterogeneousgeneticdifferentiationinalagoonspecies
AT lasotarafal nextgenerationphylogeographyofthecocklecerastodermaglaucumhighlyheterogeneousgeneticdifferentiationinalagoonspecies
AT furlapaola nextgenerationphylogeographyofthecocklecerastodermaglaucumhighlyheterogeneousgeneticdifferentiationinalagoonspecies
AT wolowiczmaciej nextgenerationphylogeographyofthecocklecerastodermaglaucumhighlyheterogeneousgeneticdifferentiationinalagoonspecies