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Phosphorothioate DNA Mediated Sequence-Insensitive Etching and Ripening of Silver Nanoparticles

Many DNA-functionalized nanomaterials and biosensors have been reported, but most have ignored the influence of DNA on the stability of nanoparticles. We observed that cytosine-rich DNA oligonucleotides can etch silver nanoparticles (AgNPs). In this work, we showed that phosphorothioate (PS)-modifie...

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Autores principales: Hu, Shengqiang, Huang, Po-Jung Jimmy, Wang, Jianxiu, Liu, Juewen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476897/
https://www.ncbi.nlm.nih.gov/pubmed/31041302
http://dx.doi.org/10.3389/fchem.2019.00198
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author Hu, Shengqiang
Huang, Po-Jung Jimmy
Wang, Jianxiu
Liu, Juewen
author_facet Hu, Shengqiang
Huang, Po-Jung Jimmy
Wang, Jianxiu
Liu, Juewen
author_sort Hu, Shengqiang
collection PubMed
description Many DNA-functionalized nanomaterials and biosensors have been reported, but most have ignored the influence of DNA on the stability of nanoparticles. We observed that cytosine-rich DNA oligonucleotides can etch silver nanoparticles (AgNPs). In this work, we showed that phosphorothioate (PS)-modified DNA (PS-DNA) can etch AgNPs independently of DNA sequence, suggesting that the thio-modifications are playing the major role in etching. Compared to unmodified DNA (e.g., poly-cytosine DNA), the concentration of required PS DNA decreases sharply, and the reaction rate increases. Furthermore, etching by PS-DNA occurs quite independent of pH, which is also different from unmodified DNA. The PS-DNA mediated etching could also be controlled well by varying DNA length and conformation, and the number and location of PS modifications. With a higher activity of PS-DNA, the process of etching, ripening, and further etching was taken place sequentially. The etching ability is inhibited by forming duplex DNA and thus etching can be used to measure the concentration of complementary DNA.
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spelling pubmed-64768972019-04-30 Phosphorothioate DNA Mediated Sequence-Insensitive Etching and Ripening of Silver Nanoparticles Hu, Shengqiang Huang, Po-Jung Jimmy Wang, Jianxiu Liu, Juewen Front Chem Chemistry Many DNA-functionalized nanomaterials and biosensors have been reported, but most have ignored the influence of DNA on the stability of nanoparticles. We observed that cytosine-rich DNA oligonucleotides can etch silver nanoparticles (AgNPs). In this work, we showed that phosphorothioate (PS)-modified DNA (PS-DNA) can etch AgNPs independently of DNA sequence, suggesting that the thio-modifications are playing the major role in etching. Compared to unmodified DNA (e.g., poly-cytosine DNA), the concentration of required PS DNA decreases sharply, and the reaction rate increases. Furthermore, etching by PS-DNA occurs quite independent of pH, which is also different from unmodified DNA. The PS-DNA mediated etching could also be controlled well by varying DNA length and conformation, and the number and location of PS modifications. With a higher activity of PS-DNA, the process of etching, ripening, and further etching was taken place sequentially. The etching ability is inhibited by forming duplex DNA and thus etching can be used to measure the concentration of complementary DNA. Frontiers Media S.A. 2019-04-16 /pmc/articles/PMC6476897/ /pubmed/31041302 http://dx.doi.org/10.3389/fchem.2019.00198 Text en Copyright © 2019 Hu, Huang, Wang and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Hu, Shengqiang
Huang, Po-Jung Jimmy
Wang, Jianxiu
Liu, Juewen
Phosphorothioate DNA Mediated Sequence-Insensitive Etching and Ripening of Silver Nanoparticles
title Phosphorothioate DNA Mediated Sequence-Insensitive Etching and Ripening of Silver Nanoparticles
title_full Phosphorothioate DNA Mediated Sequence-Insensitive Etching and Ripening of Silver Nanoparticles
title_fullStr Phosphorothioate DNA Mediated Sequence-Insensitive Etching and Ripening of Silver Nanoparticles
title_full_unstemmed Phosphorothioate DNA Mediated Sequence-Insensitive Etching and Ripening of Silver Nanoparticles
title_short Phosphorothioate DNA Mediated Sequence-Insensitive Etching and Ripening of Silver Nanoparticles
title_sort phosphorothioate dna mediated sequence-insensitive etching and ripening of silver nanoparticles
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6476897/
https://www.ncbi.nlm.nih.gov/pubmed/31041302
http://dx.doi.org/10.3389/fchem.2019.00198
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