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Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi)

OBJECTIVE: Limited sample sizes are often a problem for species of conservation concern when using genetic tools to make population assessments. Lack of analytical power from small sample sizes can be compensated for by use of a large marker set. Here we report on development and characterization of...

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Detalles Bibliográficos
Autores principales: Armstrong, Amelia J., Dudgeon, Christine L., Bustamante, Carlos, Bennett, Michael B., Ovenden, Jennifer R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6477734/
https://www.ncbi.nlm.nih.gov/pubmed/31010433
http://dx.doi.org/10.1186/s13104-019-4270-8
Descripción
Sumario:OBJECTIVE: Limited sample sizes are often a problem for species of conservation concern when using genetic tools to make population assessments. Lack of analytical power from small sample sizes can be compensated for by use of a large marker set. Here we report on development and characterization of 17 novel microsatellite markers for the reef manta ray (Mobula alfredi). RESULTS: Loci were screened on 60 reef manta rays (M. alfredi) sampled from the east coast of Australia. The number of alleles per locus varied from 2 to 13 with observed heterozygosities ranging between 0.300 and 0.917. The development of these 17 additional markers increases the total number of microsatellite markers available for this species to 27.