Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi)

OBJECTIVE: Limited sample sizes are often a problem for species of conservation concern when using genetic tools to make population assessments. Lack of analytical power from small sample sizes can be compensated for by use of a large marker set. Here we report on development and characterization of...

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Autores principales: Armstrong, Amelia J., Dudgeon, Christine L., Bustamante, Carlos, Bennett, Michael B., Ovenden, Jennifer R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6477734/
https://www.ncbi.nlm.nih.gov/pubmed/31010433
http://dx.doi.org/10.1186/s13104-019-4270-8
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author Armstrong, Amelia J.
Dudgeon, Christine L.
Bustamante, Carlos
Bennett, Michael B.
Ovenden, Jennifer R.
author_facet Armstrong, Amelia J.
Dudgeon, Christine L.
Bustamante, Carlos
Bennett, Michael B.
Ovenden, Jennifer R.
author_sort Armstrong, Amelia J.
collection PubMed
description OBJECTIVE: Limited sample sizes are often a problem for species of conservation concern when using genetic tools to make population assessments. Lack of analytical power from small sample sizes can be compensated for by use of a large marker set. Here we report on development and characterization of 17 novel microsatellite markers for the reef manta ray (Mobula alfredi). RESULTS: Loci were screened on 60 reef manta rays (M. alfredi) sampled from the east coast of Australia. The number of alleles per locus varied from 2 to 13 with observed heterozygosities ranging between 0.300 and 0.917. The development of these 17 additional markers increases the total number of microsatellite markers available for this species to 27.
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spelling pubmed-64777342019-05-01 Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi) Armstrong, Amelia J. Dudgeon, Christine L. Bustamante, Carlos Bennett, Michael B. Ovenden, Jennifer R. BMC Res Notes Research Note OBJECTIVE: Limited sample sizes are often a problem for species of conservation concern when using genetic tools to make population assessments. Lack of analytical power from small sample sizes can be compensated for by use of a large marker set. Here we report on development and characterization of 17 novel microsatellite markers for the reef manta ray (Mobula alfredi). RESULTS: Loci were screened on 60 reef manta rays (M. alfredi) sampled from the east coast of Australia. The number of alleles per locus varied from 2 to 13 with observed heterozygosities ranging between 0.300 and 0.917. The development of these 17 additional markers increases the total number of microsatellite markers available for this species to 27. BioMed Central 2019-04-22 /pmc/articles/PMC6477734/ /pubmed/31010433 http://dx.doi.org/10.1186/s13104-019-4270-8 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Armstrong, Amelia J.
Dudgeon, Christine L.
Bustamante, Carlos
Bennett, Michael B.
Ovenden, Jennifer R.
Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi)
title Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi)
title_full Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi)
title_fullStr Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi)
title_full_unstemmed Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi)
title_short Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi)
title_sort development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (mobula alfredi)
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6477734/
https://www.ncbi.nlm.nih.gov/pubmed/31010433
http://dx.doi.org/10.1186/s13104-019-4270-8
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