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Potential biomarkers to detect traumatic brain injury by the profiling of salivary extracellular vesicles
Traumatic brain injury (TBI) is a common cause of death and acquired disability in adults and children. Identifying biomarkers for mild TBI (mTBI) that can predict functional impairments on neuropsychiatric and neurocognitive testing after head trauma is yet to be firmly established. Extracellular v...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6478516/ https://www.ncbi.nlm.nih.gov/pubmed/30644102 http://dx.doi.org/10.1002/jcp.28139 |
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author | Cheng, Yan Pereira, Mandy Raukar, Neha Reagan, John L. Queseneberry, Mathew Goldberg, Laura Borgovan, Theodor LaFrance, W Curt Dooner, Mark Deregibus, Maria Camussi, Giovanni Ramratnam, Bharat Quesenberry, Peter |
author_facet | Cheng, Yan Pereira, Mandy Raukar, Neha Reagan, John L. Queseneberry, Mathew Goldberg, Laura Borgovan, Theodor LaFrance, W Curt Dooner, Mark Deregibus, Maria Camussi, Giovanni Ramratnam, Bharat Quesenberry, Peter |
author_sort | Cheng, Yan |
collection | PubMed |
description | Traumatic brain injury (TBI) is a common cause of death and acquired disability in adults and children. Identifying biomarkers for mild TBI (mTBI) that can predict functional impairments on neuropsychiatric and neurocognitive testing after head trauma is yet to be firmly established. Extracellular vesicles (EVs) are known to traffic from the brain to the oral cavity and can be detected in saliva. We hypothesize the genetic profile of salivary EVs in patients who have suffered head trauma will differ from normal healthy controls, thus constituting a unique expression signature for mTBI. We enrolled a total of 54 subjects including for saliva sampling, 23 controls with no history of head traumas, 16 patients enrolled from an outpatient concussion clinic, and 15 patients from the emergency department who had sustained a head trauma within 24 hr. We performed real‐time PCR of the salivary EVs of the 54 subjects profiling 96 genes from the TaqMan Human Alzheimer's disease array. Real‐time PCR analysis revealed 57 (15 genes, p < 0.05) upregulated genes in emergency department patients and 56 (14 genes, p < 0.05) upregulated genes in concussion clinic patients when compared with controls. Three genes were upregulated in both the emergency department patients and concussion clinic patients: CDC2, CSNK1A1, and CTSD ( p < 0.05). Our results demonstrate that salivary EVs gene expression can serve as a viable source of biomarkers for mTBI. This study shows multiple Alzheimer's disease genes present after an mTBI. |
format | Online Article Text |
id | pubmed-6478516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64785162019-08-09 Potential biomarkers to detect traumatic brain injury by the profiling of salivary extracellular vesicles Cheng, Yan Pereira, Mandy Raukar, Neha Reagan, John L. Queseneberry, Mathew Goldberg, Laura Borgovan, Theodor LaFrance, W Curt Dooner, Mark Deregibus, Maria Camussi, Giovanni Ramratnam, Bharat Quesenberry, Peter J Cell Physiol Original Research Articles Traumatic brain injury (TBI) is a common cause of death and acquired disability in adults and children. Identifying biomarkers for mild TBI (mTBI) that can predict functional impairments on neuropsychiatric and neurocognitive testing after head trauma is yet to be firmly established. Extracellular vesicles (EVs) are known to traffic from the brain to the oral cavity and can be detected in saliva. We hypothesize the genetic profile of salivary EVs in patients who have suffered head trauma will differ from normal healthy controls, thus constituting a unique expression signature for mTBI. We enrolled a total of 54 subjects including for saliva sampling, 23 controls with no history of head traumas, 16 patients enrolled from an outpatient concussion clinic, and 15 patients from the emergency department who had sustained a head trauma within 24 hr. We performed real‐time PCR of the salivary EVs of the 54 subjects profiling 96 genes from the TaqMan Human Alzheimer's disease array. Real‐time PCR analysis revealed 57 (15 genes, p < 0.05) upregulated genes in emergency department patients and 56 (14 genes, p < 0.05) upregulated genes in concussion clinic patients when compared with controls. Three genes were upregulated in both the emergency department patients and concussion clinic patients: CDC2, CSNK1A1, and CTSD ( p < 0.05). Our results demonstrate that salivary EVs gene expression can serve as a viable source of biomarkers for mTBI. This study shows multiple Alzheimer's disease genes present after an mTBI. John Wiley and Sons Inc. 2019-01-15 2019-08 /pmc/articles/PMC6478516/ /pubmed/30644102 http://dx.doi.org/10.1002/jcp.28139 Text en © 2019 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Articles Cheng, Yan Pereira, Mandy Raukar, Neha Reagan, John L. Queseneberry, Mathew Goldberg, Laura Borgovan, Theodor LaFrance, W Curt Dooner, Mark Deregibus, Maria Camussi, Giovanni Ramratnam, Bharat Quesenberry, Peter Potential biomarkers to detect traumatic brain injury by the profiling of salivary extracellular vesicles |
title | Potential biomarkers to detect traumatic brain injury by the profiling of salivary extracellular vesicles |
title_full | Potential biomarkers to detect traumatic brain injury by the profiling of salivary extracellular vesicles |
title_fullStr | Potential biomarkers to detect traumatic brain injury by the profiling of salivary extracellular vesicles |
title_full_unstemmed | Potential biomarkers to detect traumatic brain injury by the profiling of salivary extracellular vesicles |
title_short | Potential biomarkers to detect traumatic brain injury by the profiling of salivary extracellular vesicles |
title_sort | potential biomarkers to detect traumatic brain injury by the profiling of salivary extracellular vesicles |
topic | Original Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6478516/ https://www.ncbi.nlm.nih.gov/pubmed/30644102 http://dx.doi.org/10.1002/jcp.28139 |
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