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In vivo models of lung neutrophil activation. Comparison of mice and hamsters

BACKGROUND: Evidence suggests that both the migration and activation of neutrophils into the airway is of importance in pathological conditions such as pulmonary emphysema. In the present study, we describe in vivo models of lung neutrophil infiltration and activation in mice and hamsters. RESULTS:...

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Autores principales: Corteling, Randolph, Wyss, Daniel, Trifilieff, Alexandre
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC64786/
https://www.ncbi.nlm.nih.gov/pubmed/11806755
http://dx.doi.org/10.1186/1471-2210-2-1
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author Corteling, Randolph
Wyss, Daniel
Trifilieff, Alexandre
author_facet Corteling, Randolph
Wyss, Daniel
Trifilieff, Alexandre
author_sort Corteling, Randolph
collection PubMed
description BACKGROUND: Evidence suggests that both the migration and activation of neutrophils into the airway is of importance in pathological conditions such as pulmonary emphysema. In the present study, we describe in vivo models of lung neutrophil infiltration and activation in mice and hamsters. RESULTS: BALB/c and C57BL/6 mice were intranasally treated with lipopolysaccharide (0.3 mg/kg). Twenty-four hours after, animals were treated intranasally with N-Formyl-Met-Leu-Phe (0 to 5 mg/kg). Golden Syrian hamsters were treated intratracheally with 0.5 mg/kg of lipopolysaccharide. Twenty-four hours after, animals were treated intratracheally with 0.25 mg/kg of N-Formyl-Met-Leu-Phe. Both mice and hamster were sacrificed two hours after the N-Formyl-Met-Leu-Phe application. In both BALB/c and C57BL/6 mice, a neutrophil infiltration was observed after the sequential application of lipopolysaccharide and N-Formyl-Met-Leu-Phe. However, 5 times less neutrophil was found in C57BL/6 mice when compared to BALB/c mice. This was reflected in the neutrophil activation parameters measured (myeloperoxidase and elastase activities). Despite the presence of neutrophil and their activation status, no lung haemorrhage could be detected in both strains of mice. When compared with mice, the lung inflammation induced by the sequential application of lipopolysaccharide and N-Formyl-Met-Leu-Phe was much greater in the hamster. In parallel with this lung inflammation, a significant lung haemorrhage was also observed. CONCLUSIONS: Both mouse and hamster can be used for pharmacological studies of new drugs or other therapeutics agents that aimed to interfere with neutrophil activation. However, only the hamster model seems to be suitable for studying the haemorrhagic lung injury process.
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spelling pubmed-647862002-01-25 In vivo models of lung neutrophil activation. Comparison of mice and hamsters Corteling, Randolph Wyss, Daniel Trifilieff, Alexandre BMC Pharmacol Methodology Article BACKGROUND: Evidence suggests that both the migration and activation of neutrophils into the airway is of importance in pathological conditions such as pulmonary emphysema. In the present study, we describe in vivo models of lung neutrophil infiltration and activation in mice and hamsters. RESULTS: BALB/c and C57BL/6 mice were intranasally treated with lipopolysaccharide (0.3 mg/kg). Twenty-four hours after, animals were treated intranasally with N-Formyl-Met-Leu-Phe (0 to 5 mg/kg). Golden Syrian hamsters were treated intratracheally with 0.5 mg/kg of lipopolysaccharide. Twenty-four hours after, animals were treated intratracheally with 0.25 mg/kg of N-Formyl-Met-Leu-Phe. Both mice and hamster were sacrificed two hours after the N-Formyl-Met-Leu-Phe application. In both BALB/c and C57BL/6 mice, a neutrophil infiltration was observed after the sequential application of lipopolysaccharide and N-Formyl-Met-Leu-Phe. However, 5 times less neutrophil was found in C57BL/6 mice when compared to BALB/c mice. This was reflected in the neutrophil activation parameters measured (myeloperoxidase and elastase activities). Despite the presence of neutrophil and their activation status, no lung haemorrhage could be detected in both strains of mice. When compared with mice, the lung inflammation induced by the sequential application of lipopolysaccharide and N-Formyl-Met-Leu-Phe was much greater in the hamster. In parallel with this lung inflammation, a significant lung haemorrhage was also observed. CONCLUSIONS: Both mouse and hamster can be used for pharmacological studies of new drugs or other therapeutics agents that aimed to interfere with neutrophil activation. However, only the hamster model seems to be suitable for studying the haemorrhagic lung injury process. BioMed Central 2002-01-10 /pmc/articles/PMC64786/ /pubmed/11806755 http://dx.doi.org/10.1186/1471-2210-2-1 Text en Copyright © 2002 Corteling et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Methodology Article
Corteling, Randolph
Wyss, Daniel
Trifilieff, Alexandre
In vivo models of lung neutrophil activation. Comparison of mice and hamsters
title In vivo models of lung neutrophil activation. Comparison of mice and hamsters
title_full In vivo models of lung neutrophil activation. Comparison of mice and hamsters
title_fullStr In vivo models of lung neutrophil activation. Comparison of mice and hamsters
title_full_unstemmed In vivo models of lung neutrophil activation. Comparison of mice and hamsters
title_short In vivo models of lung neutrophil activation. Comparison of mice and hamsters
title_sort in vivo models of lung neutrophil activation. comparison of mice and hamsters
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC64786/
https://www.ncbi.nlm.nih.gov/pubmed/11806755
http://dx.doi.org/10.1186/1471-2210-2-1
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