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A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products
An efficient PCR cloning method is indispensable in modern molecular biology, as it can greatly improve the efficiency of DNA cloning processes. Here, I describe the development of three vectors for TA cloning and blunt-end cloning. Specifically, pCRT and pCRZeroT were designed to improve the effici...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6478821/ https://www.ncbi.nlm.nih.gov/pubmed/31015513 http://dx.doi.org/10.1038/s41598-019-42868-6 |