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Proteome data of female Anopheles stephensi antennae

Antennae of female Anopheles stephensi mosquitoes were dissected and lysed with 1% SDS. Proteins were extracted using ultra sonication and analyzed on high resolution mass spectrometer. Proteomic data was analyzed using two search algorithms SEQUEST and Mascot, resulting in the identification of 22,...

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Autores principales: Mohanty, Ajeet Kumar, Dey, Gourav, Kumar, Manish, Sreenivasamurthy, Sreelakshmi K., Garg, Sandeep, Prasad, T.S. Keshava, Kumar, Ashwani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479098/
https://www.ncbi.nlm.nih.gov/pubmed/31049374
http://dx.doi.org/10.1016/j.dib.2019.103911
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author Mohanty, Ajeet Kumar
Dey, Gourav
Kumar, Manish
Sreenivasamurthy, Sreelakshmi K.
Garg, Sandeep
Prasad, T.S. Keshava
Kumar, Ashwani
author_facet Mohanty, Ajeet Kumar
Dey, Gourav
Kumar, Manish
Sreenivasamurthy, Sreelakshmi K.
Garg, Sandeep
Prasad, T.S. Keshava
Kumar, Ashwani
author_sort Mohanty, Ajeet Kumar
collection PubMed
description Antennae of female Anopheles stephensi mosquitoes were dissected and lysed with 1% SDS. Proteins were extracted using ultra sonication and analyzed on high resolution mass spectrometer. Proteomic data was analyzed using two search algorithms SEQUEST and Mascot, resulting in the identification of 22,729 peptides corresponding to 3262 proteins. These proteins were characterized using different bioinformatics tools. VectorBase resource was used to assign Gene Ontology (GO) terms. Using Biomart tool ortholog information was fetched from the VectorBase database. Raw mass spectrometric data was deposited in ProteomeXchange Consortium via PRIDE partner repository in the public dataset PXD001128. Proteins involved in insecticide resistance and odorant binding were the most abundant in the antennae. The proteins identified in this study could be targeted for developing novel vector control strategy.
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spelling pubmed-64790982019-05-02 Proteome data of female Anopheles stephensi antennae Mohanty, Ajeet Kumar Dey, Gourav Kumar, Manish Sreenivasamurthy, Sreelakshmi K. Garg, Sandeep Prasad, T.S. Keshava Kumar, Ashwani Data Brief Biochemistry, Genetics and Molecular Biology Antennae of female Anopheles stephensi mosquitoes were dissected and lysed with 1% SDS. Proteins were extracted using ultra sonication and analyzed on high resolution mass spectrometer. Proteomic data was analyzed using two search algorithms SEQUEST and Mascot, resulting in the identification of 22,729 peptides corresponding to 3262 proteins. These proteins were characterized using different bioinformatics tools. VectorBase resource was used to assign Gene Ontology (GO) terms. Using Biomart tool ortholog information was fetched from the VectorBase database. Raw mass spectrometric data was deposited in ProteomeXchange Consortium via PRIDE partner repository in the public dataset PXD001128. Proteins involved in insecticide resistance and odorant binding were the most abundant in the antennae. The proteins identified in this study could be targeted for developing novel vector control strategy. Elsevier 2019-04-08 /pmc/articles/PMC6479098/ /pubmed/31049374 http://dx.doi.org/10.1016/j.dib.2019.103911 Text en © 2019 Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Mohanty, Ajeet Kumar
Dey, Gourav
Kumar, Manish
Sreenivasamurthy, Sreelakshmi K.
Garg, Sandeep
Prasad, T.S. Keshava
Kumar, Ashwani
Proteome data of female Anopheles stephensi antennae
title Proteome data of female Anopheles stephensi antennae
title_full Proteome data of female Anopheles stephensi antennae
title_fullStr Proteome data of female Anopheles stephensi antennae
title_full_unstemmed Proteome data of female Anopheles stephensi antennae
title_short Proteome data of female Anopheles stephensi antennae
title_sort proteome data of female anopheles stephensi antennae
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479098/
https://www.ncbi.nlm.nih.gov/pubmed/31049374
http://dx.doi.org/10.1016/j.dib.2019.103911
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