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Proteome data of female Anopheles stephensi antennae
Antennae of female Anopheles stephensi mosquitoes were dissected and lysed with 1% SDS. Proteins were extracted using ultra sonication and analyzed on high resolution mass spectrometer. Proteomic data was analyzed using two search algorithms SEQUEST and Mascot, resulting in the identification of 22,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479098/ https://www.ncbi.nlm.nih.gov/pubmed/31049374 http://dx.doi.org/10.1016/j.dib.2019.103911 |
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author | Mohanty, Ajeet Kumar Dey, Gourav Kumar, Manish Sreenivasamurthy, Sreelakshmi K. Garg, Sandeep Prasad, T.S. Keshava Kumar, Ashwani |
author_facet | Mohanty, Ajeet Kumar Dey, Gourav Kumar, Manish Sreenivasamurthy, Sreelakshmi K. Garg, Sandeep Prasad, T.S. Keshava Kumar, Ashwani |
author_sort | Mohanty, Ajeet Kumar |
collection | PubMed |
description | Antennae of female Anopheles stephensi mosquitoes were dissected and lysed with 1% SDS. Proteins were extracted using ultra sonication and analyzed on high resolution mass spectrometer. Proteomic data was analyzed using two search algorithms SEQUEST and Mascot, resulting in the identification of 22,729 peptides corresponding to 3262 proteins. These proteins were characterized using different bioinformatics tools. VectorBase resource was used to assign Gene Ontology (GO) terms. Using Biomart tool ortholog information was fetched from the VectorBase database. Raw mass spectrometric data was deposited in ProteomeXchange Consortium via PRIDE partner repository in the public dataset PXD001128. Proteins involved in insecticide resistance and odorant binding were the most abundant in the antennae. The proteins identified in this study could be targeted for developing novel vector control strategy. |
format | Online Article Text |
id | pubmed-6479098 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-64790982019-05-02 Proteome data of female Anopheles stephensi antennae Mohanty, Ajeet Kumar Dey, Gourav Kumar, Manish Sreenivasamurthy, Sreelakshmi K. Garg, Sandeep Prasad, T.S. Keshava Kumar, Ashwani Data Brief Biochemistry, Genetics and Molecular Biology Antennae of female Anopheles stephensi mosquitoes were dissected and lysed with 1% SDS. Proteins were extracted using ultra sonication and analyzed on high resolution mass spectrometer. Proteomic data was analyzed using two search algorithms SEQUEST and Mascot, resulting in the identification of 22,729 peptides corresponding to 3262 proteins. These proteins were characterized using different bioinformatics tools. VectorBase resource was used to assign Gene Ontology (GO) terms. Using Biomart tool ortholog information was fetched from the VectorBase database. Raw mass spectrometric data was deposited in ProteomeXchange Consortium via PRIDE partner repository in the public dataset PXD001128. Proteins involved in insecticide resistance and odorant binding were the most abundant in the antennae. The proteins identified in this study could be targeted for developing novel vector control strategy. Elsevier 2019-04-08 /pmc/articles/PMC6479098/ /pubmed/31049374 http://dx.doi.org/10.1016/j.dib.2019.103911 Text en © 2019 Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Biochemistry, Genetics and Molecular Biology Mohanty, Ajeet Kumar Dey, Gourav Kumar, Manish Sreenivasamurthy, Sreelakshmi K. Garg, Sandeep Prasad, T.S. Keshava Kumar, Ashwani Proteome data of female Anopheles stephensi antennae |
title | Proteome data of female Anopheles stephensi antennae |
title_full | Proteome data of female Anopheles stephensi antennae |
title_fullStr | Proteome data of female Anopheles stephensi antennae |
title_full_unstemmed | Proteome data of female Anopheles stephensi antennae |
title_short | Proteome data of female Anopheles stephensi antennae |
title_sort | proteome data of female anopheles stephensi antennae |
topic | Biochemistry, Genetics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479098/ https://www.ncbi.nlm.nih.gov/pubmed/31049374 http://dx.doi.org/10.1016/j.dib.2019.103911 |
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