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Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components

We aimed to purify polyphenols from distiller’s grain extract using macroporous resins and to identify its polyphenolic components. The influence of operational parameters on purification efficiency was investigated. The polyphenolic composition was analyzed by ultra-performance liquid chromatograph...

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Detalles Bibliográficos
Autores principales: Wang, Xiaoyuan, Wang, Shuangshuang, Huang, Shasha, Zhang, Lihua, Ge, Zhenzhen, Sun, Liping, Zong, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479346/
https://www.ncbi.nlm.nih.gov/pubmed/30986967
http://dx.doi.org/10.3390/molecules24071284
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author Wang, Xiaoyuan
Wang, Shuangshuang
Huang, Shasha
Zhang, Lihua
Ge, Zhenzhen
Sun, Liping
Zong, Wei
author_facet Wang, Xiaoyuan
Wang, Shuangshuang
Huang, Shasha
Zhang, Lihua
Ge, Zhenzhen
Sun, Liping
Zong, Wei
author_sort Wang, Xiaoyuan
collection PubMed
description We aimed to purify polyphenols from distiller’s grain extract using macroporous resins and to identify its polyphenolic components. The influence of operational parameters on purification efficiency was investigated. The polyphenolic composition was analyzed by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) and then quantified by UPLC-MS using authenticated standards. The results showed that the optimal purifying conditions were D101 resin with a dosage of 3 g, four hours adsorption, three hours desorption time, and 60% ethanol as the eluent, producing the highest purification rate of 51%. The purified distiller’s grain extract exhibited stronger antioxidant activity than the unpurified extracts, which was assessed using DPPH and ABTS methods (IC(50) DPPH = 34.03 and 16.21 μg/mL, respectively; IC(50) ABTS = 20.31 and 5.73 μg/mL, respectively). UPLC-MS results indicated that (−)-epicatechin is the major compound found in distiller’s grain extract which was quantified as 562.7 μg/g extract, followed by ferulic acid (518.2 μg/g), p-hydroxybenzoic acid (417.7 μg/g), caffeic acid (217.1 μg/g), syringic acid (158.0 μg/g) and quercetin (147.8 μg/g). Two compounds, vanillic acid (66.5 μg/g) and gallic acid (41.4 μg/g), were found in lower concentrations. The findings of this study suggest that purification of polyphenolic compounds from distiller’s grain by macroporous resins is feasible, providing a new and effective method for the secondary use of distiller’s grain resources.
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spelling pubmed-64793462019-04-30 Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components Wang, Xiaoyuan Wang, Shuangshuang Huang, Shasha Zhang, Lihua Ge, Zhenzhen Sun, Liping Zong, Wei Molecules Article We aimed to purify polyphenols from distiller’s grain extract using macroporous resins and to identify its polyphenolic components. The influence of operational parameters on purification efficiency was investigated. The polyphenolic composition was analyzed by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) and then quantified by UPLC-MS using authenticated standards. The results showed that the optimal purifying conditions were D101 resin with a dosage of 3 g, four hours adsorption, three hours desorption time, and 60% ethanol as the eluent, producing the highest purification rate of 51%. The purified distiller’s grain extract exhibited stronger antioxidant activity than the unpurified extracts, which was assessed using DPPH and ABTS methods (IC(50) DPPH = 34.03 and 16.21 μg/mL, respectively; IC(50) ABTS = 20.31 and 5.73 μg/mL, respectively). UPLC-MS results indicated that (−)-epicatechin is the major compound found in distiller’s grain extract which was quantified as 562.7 μg/g extract, followed by ferulic acid (518.2 μg/g), p-hydroxybenzoic acid (417.7 μg/g), caffeic acid (217.1 μg/g), syringic acid (158.0 μg/g) and quercetin (147.8 μg/g). Two compounds, vanillic acid (66.5 μg/g) and gallic acid (41.4 μg/g), were found in lower concentrations. The findings of this study suggest that purification of polyphenolic compounds from distiller’s grain by macroporous resins is feasible, providing a new and effective method for the secondary use of distiller’s grain resources. MDPI 2019-04-02 /pmc/articles/PMC6479346/ /pubmed/30986967 http://dx.doi.org/10.3390/molecules24071284 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Xiaoyuan
Wang, Shuangshuang
Huang, Shasha
Zhang, Lihua
Ge, Zhenzhen
Sun, Liping
Zong, Wei
Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components
title Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components
title_full Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components
title_fullStr Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components
title_full_unstemmed Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components
title_short Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components
title_sort purification of polyphenols from distiller’s grains by macroporous resin and analysis of the polyphenolic components
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479346/
https://www.ncbi.nlm.nih.gov/pubmed/30986967
http://dx.doi.org/10.3390/molecules24071284
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