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Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology
A methodology was implemented for purifying peptides in one chromatographic run via solid-phase extraction (SPE), reverse phase mode (RP), and gradient elution, obtaining high-purity products with good yields. Crude peptides were analyzed by reverse phase high performance liquid chromatography and a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479624/ https://www.ncbi.nlm.nih.gov/pubmed/30925720 http://dx.doi.org/10.3390/molecules24071215 |
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author | Insuasty Cepeda, Diego Sebastián Pineda Castañeda, Héctor Manuel Rodríguez Mayor, Andrea Verónica García Castañeda, Javier Eduardo Maldonado Villamil, Mauricio Fierro Medina, Ricardo Rivera Monroy, Zuly Jenny |
author_facet | Insuasty Cepeda, Diego Sebastián Pineda Castañeda, Héctor Manuel Rodríguez Mayor, Andrea Verónica García Castañeda, Javier Eduardo Maldonado Villamil, Mauricio Fierro Medina, Ricardo Rivera Monroy, Zuly Jenny |
author_sort | Insuasty Cepeda, Diego Sebastián |
collection | PubMed |
description | A methodology was implemented for purifying peptides in one chromatographic run via solid-phase extraction (SPE), reverse phase mode (RP), and gradient elution, obtaining high-purity products with good yields. Crude peptides were analyzed by reverse phase high performance liquid chromatography and a new mathematical model based on its retention time was developed in order to predict the percentage of organic modifier in which the peptide will elute in RP-SPE. This information was used for designing the elution program of each molecule. It was possible to purify peptides with different physicochemical properties, showing that this method is versatile and requires low solvent consumption, making it the least polluting one. Reverse phase-SPE can easily be routinely implemented. It is an alternative to enrich and purified synthetic or natural molecules. |
format | Online Article Text |
id | pubmed-6479624 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64796242019-04-30 Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology Insuasty Cepeda, Diego Sebastián Pineda Castañeda, Héctor Manuel Rodríguez Mayor, Andrea Verónica García Castañeda, Javier Eduardo Maldonado Villamil, Mauricio Fierro Medina, Ricardo Rivera Monroy, Zuly Jenny Molecules Article A methodology was implemented for purifying peptides in one chromatographic run via solid-phase extraction (SPE), reverse phase mode (RP), and gradient elution, obtaining high-purity products with good yields. Crude peptides were analyzed by reverse phase high performance liquid chromatography and a new mathematical model based on its retention time was developed in order to predict the percentage of organic modifier in which the peptide will elute in RP-SPE. This information was used for designing the elution program of each molecule. It was possible to purify peptides with different physicochemical properties, showing that this method is versatile and requires low solvent consumption, making it the least polluting one. Reverse phase-SPE can easily be routinely implemented. It is an alternative to enrich and purified synthetic or natural molecules. MDPI 2019-03-28 /pmc/articles/PMC6479624/ /pubmed/30925720 http://dx.doi.org/10.3390/molecules24071215 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Insuasty Cepeda, Diego Sebastián Pineda Castañeda, Héctor Manuel Rodríguez Mayor, Andrea Verónica García Castañeda, Javier Eduardo Maldonado Villamil, Mauricio Fierro Medina, Ricardo Rivera Monroy, Zuly Jenny Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology |
title | Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology |
title_full | Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology |
title_fullStr | Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology |
title_full_unstemmed | Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology |
title_short | Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology |
title_sort | synthetic peptide purification via solid-phase extraction with gradient elution: a simple, economical, fast, and efficient methodology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479624/ https://www.ncbi.nlm.nih.gov/pubmed/30925720 http://dx.doi.org/10.3390/molecules24071215 |
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