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Analysis of Flavonoid Metabolites in Buckwheat Leaves Using UPLC-ESI-MS/MS

Flavonoids from plants are particularly important in our diet. Buckwheat is a special crop that is rich in flavonoids. In this study, four important buckwheat varieties, including one tartary buckwheat and three common buckwheat varieties, were selected as experimental materials. The total flavonoid...

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Autores principales: Li, Jing, Yang, Pu, Yang, Qinghua, Gong, Xiangwei, Ma, Hongchi, Dang, Ke, Chen, Guanghua, Gao, Xiaoli, Feng, Baili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479795/
https://www.ncbi.nlm.nih.gov/pubmed/30987158
http://dx.doi.org/10.3390/molecules24071310
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author Li, Jing
Yang, Pu
Yang, Qinghua
Gong, Xiangwei
Ma, Hongchi
Dang, Ke
Chen, Guanghua
Gao, Xiaoli
Feng, Baili
author_facet Li, Jing
Yang, Pu
Yang, Qinghua
Gong, Xiangwei
Ma, Hongchi
Dang, Ke
Chen, Guanghua
Gao, Xiaoli
Feng, Baili
author_sort Li, Jing
collection PubMed
description Flavonoids from plants are particularly important in our diet. Buckwheat is a special crop that is rich in flavonoids. In this study, four important buckwheat varieties, including one tartary buckwheat and three common buckwheat varieties, were selected as experimental materials. The total flavonoid content of leaves from red-flowered common buckwheat was the highest, followed by tartary buckwheat leaves. A total of 182 flavonoid metabolites (including 53 flavone, 37 flavonol, 32 flavone C-glycosides, 24 flavanone, 18 anthocyanins, 7 isoflavone, 6 flavonolignan, and 5 proanthocyanidins) were identified based on Ultra Performance Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry (UPLC-ESI-MS/MS) system. Through clustering analysis, principal component analysis (PCA), and orthogonal signal correction and partial least squares-discriminant analysis (OPLS-DA), different samples were clearly separated. Considerable differences were observed in the flavonoid metabolites between tartary buckwheat leaves and common buckwheat leaves, and both displayed unique metabolites with important biological functions. This study provides new insights into the differences of flavonoid metabolites between tartary buckwheat and common buckwheat leaves and provides theoretical basis for the sufficient utilization of buckwheat.
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spelling pubmed-64797952019-04-30 Analysis of Flavonoid Metabolites in Buckwheat Leaves Using UPLC-ESI-MS/MS Li, Jing Yang, Pu Yang, Qinghua Gong, Xiangwei Ma, Hongchi Dang, Ke Chen, Guanghua Gao, Xiaoli Feng, Baili Molecules Article Flavonoids from plants are particularly important in our diet. Buckwheat is a special crop that is rich in flavonoids. In this study, four important buckwheat varieties, including one tartary buckwheat and three common buckwheat varieties, were selected as experimental materials. The total flavonoid content of leaves from red-flowered common buckwheat was the highest, followed by tartary buckwheat leaves. A total of 182 flavonoid metabolites (including 53 flavone, 37 flavonol, 32 flavone C-glycosides, 24 flavanone, 18 anthocyanins, 7 isoflavone, 6 flavonolignan, and 5 proanthocyanidins) were identified based on Ultra Performance Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry (UPLC-ESI-MS/MS) system. Through clustering analysis, principal component analysis (PCA), and orthogonal signal correction and partial least squares-discriminant analysis (OPLS-DA), different samples were clearly separated. Considerable differences were observed in the flavonoid metabolites between tartary buckwheat leaves and common buckwheat leaves, and both displayed unique metabolites with important biological functions. This study provides new insights into the differences of flavonoid metabolites between tartary buckwheat and common buckwheat leaves and provides theoretical basis for the sufficient utilization of buckwheat. MDPI 2019-04-03 /pmc/articles/PMC6479795/ /pubmed/30987158 http://dx.doi.org/10.3390/molecules24071310 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Jing
Yang, Pu
Yang, Qinghua
Gong, Xiangwei
Ma, Hongchi
Dang, Ke
Chen, Guanghua
Gao, Xiaoli
Feng, Baili
Analysis of Flavonoid Metabolites in Buckwheat Leaves Using UPLC-ESI-MS/MS
title Analysis of Flavonoid Metabolites in Buckwheat Leaves Using UPLC-ESI-MS/MS
title_full Analysis of Flavonoid Metabolites in Buckwheat Leaves Using UPLC-ESI-MS/MS
title_fullStr Analysis of Flavonoid Metabolites in Buckwheat Leaves Using UPLC-ESI-MS/MS
title_full_unstemmed Analysis of Flavonoid Metabolites in Buckwheat Leaves Using UPLC-ESI-MS/MS
title_short Analysis of Flavonoid Metabolites in Buckwheat Leaves Using UPLC-ESI-MS/MS
title_sort analysis of flavonoid metabolites in buckwheat leaves using uplc-esi-ms/ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479795/
https://www.ncbi.nlm.nih.gov/pubmed/30987158
http://dx.doi.org/10.3390/molecules24071310
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