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Anisotropic Gold Nanoparticle-Cell Interactions Mediated by Collagen
Gold nanoparticles (AuNPs) are the groundwork of a large variety of applications in the biomedical field. Further development and a better understanding of this versatile platform will lead to an expansion of potential applications. In this study, we propose a facile synthesis of AuNPs using hydroge...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480049/ https://www.ncbi.nlm.nih.gov/pubmed/30959932 http://dx.doi.org/10.3390/ma12071131 |
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author | Marișca, Oana T. Leopold, Nicolae |
author_facet | Marișca, Oana T. Leopold, Nicolae |
author_sort | Marișca, Oana T. |
collection | PubMed |
description | Gold nanoparticles (AuNPs) are the groundwork of a large variety of applications in the biomedical field. Further development and a better understanding of this versatile platform will lead to an expansion of potential applications. In this study, we propose a facile synthesis of AuNPs using hydrogen peroxide as a reducing agent and collagen as a stabilizing agent. Our synthetic approach results in “raspberry”-like AuNPs with a mean diameter of 60 nm, as revealed by electron microscopy. The optical properties of the AuNPs were assessed by UV-Vis and surface-enhanced Raman scattering (SERS), and their stability and in vitro cytotoxicity were evaluated as well. HeLa cell viability values were only modestly affected compared to control, with the highest concentration tested displaying a 20% decrease in cellular viability. The dose-dependent cellular internalization in the 20–60 nM range indicate the highest internalization rate at 60 nM and uptake values as high as 35%. This result correlated well with the viability results. These type of anisotropic AuNPs are proposed for biomedical applications such as hyperthermia, contrast agents or imaging. Therefore, our findings offer a platform for potential biological applications such as sensing and imaging, due to their unique physico-chemical features. |
format | Online Article Text |
id | pubmed-6480049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64800492019-04-29 Anisotropic Gold Nanoparticle-Cell Interactions Mediated by Collagen Marișca, Oana T. Leopold, Nicolae Materials (Basel) Article Gold nanoparticles (AuNPs) are the groundwork of a large variety of applications in the biomedical field. Further development and a better understanding of this versatile platform will lead to an expansion of potential applications. In this study, we propose a facile synthesis of AuNPs using hydrogen peroxide as a reducing agent and collagen as a stabilizing agent. Our synthetic approach results in “raspberry”-like AuNPs with a mean diameter of 60 nm, as revealed by electron microscopy. The optical properties of the AuNPs were assessed by UV-Vis and surface-enhanced Raman scattering (SERS), and their stability and in vitro cytotoxicity were evaluated as well. HeLa cell viability values were only modestly affected compared to control, with the highest concentration tested displaying a 20% decrease in cellular viability. The dose-dependent cellular internalization in the 20–60 nM range indicate the highest internalization rate at 60 nM and uptake values as high as 35%. This result correlated well with the viability results. These type of anisotropic AuNPs are proposed for biomedical applications such as hyperthermia, contrast agents or imaging. Therefore, our findings offer a platform for potential biological applications such as sensing and imaging, due to their unique physico-chemical features. MDPI 2019-04-06 /pmc/articles/PMC6480049/ /pubmed/30959932 http://dx.doi.org/10.3390/ma12071131 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Marișca, Oana T. Leopold, Nicolae Anisotropic Gold Nanoparticle-Cell Interactions Mediated by Collagen |
title | Anisotropic Gold Nanoparticle-Cell Interactions Mediated by Collagen |
title_full | Anisotropic Gold Nanoparticle-Cell Interactions Mediated by Collagen |
title_fullStr | Anisotropic Gold Nanoparticle-Cell Interactions Mediated by Collagen |
title_full_unstemmed | Anisotropic Gold Nanoparticle-Cell Interactions Mediated by Collagen |
title_short | Anisotropic Gold Nanoparticle-Cell Interactions Mediated by Collagen |
title_sort | anisotropic gold nanoparticle-cell interactions mediated by collagen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480049/ https://www.ncbi.nlm.nih.gov/pubmed/30959932 http://dx.doi.org/10.3390/ma12071131 |
work_keys_str_mv | AT mariscaoanat anisotropicgoldnanoparticlecellinteractionsmediatedbycollagen AT leopoldnicolae anisotropicgoldnanoparticlecellinteractionsmediatedbycollagen |