Cargando…

Co-Existence of Hypertensive and Anti-Hypertensive Constituents, Synephrine, and Nobiletin in Citrus unshiu Peel

A single herb can contain multiple constituents with diverse bioactivities. We found that the extract of Citrus unshiu peel (CUP), induced abnormal vasoconstriction responses on the freshly isolated rat aortic rings in vitro. CUP stimulated the vasoconstriction alone, and it suppressed the phenyleph...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Jung-Joon, Kim, Keunyoung, Jung, Ye-Ryeon, Bian, Yiying, Ngo, Thien, Bae, Ok-Nam, Lim, Kyung-Min, Chung, Jin-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480055/
https://www.ncbi.nlm.nih.gov/pubmed/30934724
http://dx.doi.org/10.3390/molecules24071197
_version_ 1783413488068067328
author Kim, Jung-Joon
Kim, Keunyoung
Jung, Ye-Ryeon
Bian, Yiying
Ngo, Thien
Bae, Ok-Nam
Lim, Kyung-Min
Chung, Jin-Ho
author_facet Kim, Jung-Joon
Kim, Keunyoung
Jung, Ye-Ryeon
Bian, Yiying
Ngo, Thien
Bae, Ok-Nam
Lim, Kyung-Min
Chung, Jin-Ho
author_sort Kim, Jung-Joon
collection PubMed
description A single herb can contain multiple constituents with diverse bioactivities. We found that the extract of Citrus unshiu peel (CUP), induced abnormal vasoconstriction responses on the freshly isolated rat aortic rings in vitro. CUP stimulated the vasoconstriction alone, and it suppressed the phenylephrine-stimulated vasoconstriction. We studied the reasons behind this abnormal vasoconstriction pattern. Major constituents of CUP were determined and evaluated for their vaso-activities. Notably, synephrine, a contractile agonist, and nobiletin, newly identified to have anti-contractile activity co-existed in CUP. Synephrine and nobiletin competitively blocked or activated the same contractile targets resulting in contradicting and abnormal vasoconstriction responses. Accordingly, the vasoconstriction pattern varies significantly depending on the relative contents of synephrine and nobiletin in CUP. Interestingly, this response pattern could be observed with another plant extract, Acorus gramineus Sol. Collectively, we demonstrated that active ingredients with contradicting bioactivities could co-exist in a single plant extract, interact and produce abnormal response patterns in bioassay, which would give an important insight into the interpretation of unusual activity patterns induced by plant extracts.
format Online
Article
Text
id pubmed-6480055
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64800552019-04-30 Co-Existence of Hypertensive and Anti-Hypertensive Constituents, Synephrine, and Nobiletin in Citrus unshiu Peel Kim, Jung-Joon Kim, Keunyoung Jung, Ye-Ryeon Bian, Yiying Ngo, Thien Bae, Ok-Nam Lim, Kyung-Min Chung, Jin-Ho Molecules Communication A single herb can contain multiple constituents with diverse bioactivities. We found that the extract of Citrus unshiu peel (CUP), induced abnormal vasoconstriction responses on the freshly isolated rat aortic rings in vitro. CUP stimulated the vasoconstriction alone, and it suppressed the phenylephrine-stimulated vasoconstriction. We studied the reasons behind this abnormal vasoconstriction pattern. Major constituents of CUP were determined and evaluated for their vaso-activities. Notably, synephrine, a contractile agonist, and nobiletin, newly identified to have anti-contractile activity co-existed in CUP. Synephrine and nobiletin competitively blocked or activated the same contractile targets resulting in contradicting and abnormal vasoconstriction responses. Accordingly, the vasoconstriction pattern varies significantly depending on the relative contents of synephrine and nobiletin in CUP. Interestingly, this response pattern could be observed with another plant extract, Acorus gramineus Sol. Collectively, we demonstrated that active ingredients with contradicting bioactivities could co-exist in a single plant extract, interact and produce abnormal response patterns in bioassay, which would give an important insight into the interpretation of unusual activity patterns induced by plant extracts. MDPI 2019-03-27 /pmc/articles/PMC6480055/ /pubmed/30934724 http://dx.doi.org/10.3390/molecules24071197 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Kim, Jung-Joon
Kim, Keunyoung
Jung, Ye-Ryeon
Bian, Yiying
Ngo, Thien
Bae, Ok-Nam
Lim, Kyung-Min
Chung, Jin-Ho
Co-Existence of Hypertensive and Anti-Hypertensive Constituents, Synephrine, and Nobiletin in Citrus unshiu Peel
title Co-Existence of Hypertensive and Anti-Hypertensive Constituents, Synephrine, and Nobiletin in Citrus unshiu Peel
title_full Co-Existence of Hypertensive and Anti-Hypertensive Constituents, Synephrine, and Nobiletin in Citrus unshiu Peel
title_fullStr Co-Existence of Hypertensive and Anti-Hypertensive Constituents, Synephrine, and Nobiletin in Citrus unshiu Peel
title_full_unstemmed Co-Existence of Hypertensive and Anti-Hypertensive Constituents, Synephrine, and Nobiletin in Citrus unshiu Peel
title_short Co-Existence of Hypertensive and Anti-Hypertensive Constituents, Synephrine, and Nobiletin in Citrus unshiu Peel
title_sort co-existence of hypertensive and anti-hypertensive constituents, synephrine, and nobiletin in citrus unshiu peel
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480055/
https://www.ncbi.nlm.nih.gov/pubmed/30934724
http://dx.doi.org/10.3390/molecules24071197
work_keys_str_mv AT kimjungjoon coexistenceofhypertensiveandantihypertensiveconstituentssynephrineandnobiletinincitrusunshiupeel
AT kimkeunyoung coexistenceofhypertensiveandantihypertensiveconstituentssynephrineandnobiletinincitrusunshiupeel
AT jungyeryeon coexistenceofhypertensiveandantihypertensiveconstituentssynephrineandnobiletinincitrusunshiupeel
AT bianyiying coexistenceofhypertensiveandantihypertensiveconstituentssynephrineandnobiletinincitrusunshiupeel
AT ngothien coexistenceofhypertensiveandantihypertensiveconstituentssynephrineandnobiletinincitrusunshiupeel
AT baeoknam coexistenceofhypertensiveandantihypertensiveconstituentssynephrineandnobiletinincitrusunshiupeel
AT limkyungmin coexistenceofhypertensiveandantihypertensiveconstituentssynephrineandnobiletinincitrusunshiupeel
AT chungjinho coexistenceofhypertensiveandantihypertensiveconstituentssynephrineandnobiletinincitrusunshiupeel