In Vitro Toxicity Study of a Porous Iron(III) Metal‒Organic Framework

A MIL series metal‒organic framework (MOF), MIL-100(Fe), was successfully synthesized at the nanoscale and fully characterized by TEM, TGA, XRD, FTIR, DLS, and BET. A toxicological assessment was performed using two different cell lines: human normal liver cells (HL-7702) and hepatocellular carcinom...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Gongsen, Leng, Xin, Luo, Juyuan, You, Longtai, Qu, Changhai, Dong, Xiaoxv, Huang, Hongliang, Yin, Xingbin, Ni, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480057/
https://www.ncbi.nlm.nih.gov/pubmed/30925694
http://dx.doi.org/10.3390/molecules24071211
_version_ 1783413488531537920
author Chen, Gongsen
Leng, Xin
Luo, Juyuan
You, Longtai
Qu, Changhai
Dong, Xiaoxv
Huang, Hongliang
Yin, Xingbin
Ni, Jian
author_facet Chen, Gongsen
Leng, Xin
Luo, Juyuan
You, Longtai
Qu, Changhai
Dong, Xiaoxv
Huang, Hongliang
Yin, Xingbin
Ni, Jian
author_sort Chen, Gongsen
collection PubMed
description A MIL series metal‒organic framework (MOF), MIL-100(Fe), was successfully synthesized at the nanoscale and fully characterized by TEM, TGA, XRD, FTIR, DLS, and BET. A toxicological assessment was performed using two different cell lines: human normal liver cells (HL-7702) and hepatocellular carcinoma (HepG2). In vitro cytotoxicity of MIL-100(Fe) was evaluated by the MTT assay, LDH releasing rate assay, DAPI staining, and annexin V/PI double staining assay. The safe dose of MIL-100(Fe) was 80 μg/mL. It exhibited good biocompatibility, low cytotoxicity, and high cell survival rate (HL-7702 cells’ viability >85.97%, HepG2 cells’ viability >91.20%). Therefore, MIL-100(Fe) has a potential application as a drug carrier.
format Online
Article
Text
id pubmed-6480057
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64800572019-04-30 In Vitro Toxicity Study of a Porous Iron(III) Metal‒Organic Framework Chen, Gongsen Leng, Xin Luo, Juyuan You, Longtai Qu, Changhai Dong, Xiaoxv Huang, Hongliang Yin, Xingbin Ni, Jian Molecules Article A MIL series metal‒organic framework (MOF), MIL-100(Fe), was successfully synthesized at the nanoscale and fully characterized by TEM, TGA, XRD, FTIR, DLS, and BET. A toxicological assessment was performed using two different cell lines: human normal liver cells (HL-7702) and hepatocellular carcinoma (HepG2). In vitro cytotoxicity of MIL-100(Fe) was evaluated by the MTT assay, LDH releasing rate assay, DAPI staining, and annexin V/PI double staining assay. The safe dose of MIL-100(Fe) was 80 μg/mL. It exhibited good biocompatibility, low cytotoxicity, and high cell survival rate (HL-7702 cells’ viability >85.97%, HepG2 cells’ viability >91.20%). Therefore, MIL-100(Fe) has a potential application as a drug carrier. MDPI 2019-03-28 /pmc/articles/PMC6480057/ /pubmed/30925694 http://dx.doi.org/10.3390/molecules24071211 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Gongsen
Leng, Xin
Luo, Juyuan
You, Longtai
Qu, Changhai
Dong, Xiaoxv
Huang, Hongliang
Yin, Xingbin
Ni, Jian
In Vitro Toxicity Study of a Porous Iron(III) Metal‒Organic Framework
title In Vitro Toxicity Study of a Porous Iron(III) Metal‒Organic Framework
title_full In Vitro Toxicity Study of a Porous Iron(III) Metal‒Organic Framework
title_fullStr In Vitro Toxicity Study of a Porous Iron(III) Metal‒Organic Framework
title_full_unstemmed In Vitro Toxicity Study of a Porous Iron(III) Metal‒Organic Framework
title_short In Vitro Toxicity Study of a Porous Iron(III) Metal‒Organic Framework
title_sort in vitro toxicity study of a porous iron(iii) metal‒organic framework
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480057/
https://www.ncbi.nlm.nih.gov/pubmed/30925694
http://dx.doi.org/10.3390/molecules24071211
work_keys_str_mv AT chengongsen invitrotoxicitystudyofaporousironiiimetalorganicframework
AT lengxin invitrotoxicitystudyofaporousironiiimetalorganicframework
AT luojuyuan invitrotoxicitystudyofaporousironiiimetalorganicframework
AT youlongtai invitrotoxicitystudyofaporousironiiimetalorganicframework
AT quchanghai invitrotoxicitystudyofaporousironiiimetalorganicframework
AT dongxiaoxv invitrotoxicitystudyofaporousironiiimetalorganicframework
AT huanghongliang invitrotoxicitystudyofaporousironiiimetalorganicframework
AT yinxingbin invitrotoxicitystudyofaporousironiiimetalorganicframework
AT nijian invitrotoxicitystudyofaporousironiiimetalorganicframework

Ejemplares similares