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Gene Expression in Developmental Stages of Schistosoma japonicum Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide

We showed previously that the Schistosoma japonicum insulin-like peptide (SjILP) binds the worm insulin receptors, thereby, activating the parasite’s insulin pathway and emphasizing its important role in regulating uptake of glucose, a nutrient essential for parasite survival. Here we show that SjIL...

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Autores principales: Du, Xiaofeng, Jones, Malcolm K., Nawaratna, Sujeevi S. K., Ranasinghe, Shiwanthi, Xiong, Chunrong, Cai, Pengfei, McManus, Donald P., You, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480100/
https://www.ncbi.nlm.nih.gov/pubmed/30925781
http://dx.doi.org/10.3390/ijms20071565
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author Du, Xiaofeng
Jones, Malcolm K.
Nawaratna, Sujeevi S. K.
Ranasinghe, Shiwanthi
Xiong, Chunrong
Cai, Pengfei
McManus, Donald P.
You, Hong
author_facet Du, Xiaofeng
Jones, Malcolm K.
Nawaratna, Sujeevi S. K.
Ranasinghe, Shiwanthi
Xiong, Chunrong
Cai, Pengfei
McManus, Donald P.
You, Hong
author_sort Du, Xiaofeng
collection PubMed
description We showed previously that the Schistosoma japonicum insulin-like peptide (SjILP) binds the worm insulin receptors, thereby, activating the parasite’s insulin pathway and emphasizing its important role in regulating uptake of glucose, a nutrient essential for parasite survival. Here we show that SjILP is differentially expressed in the schistosome life cycle and is especially highly transcribed in eggs, miracidia, and adult female worms. RNA inference was employed to knockdown SjILP in adults in vitro, with suppression confirmed by significantly reduced protein production, declined adenosine diphosphate levels, and reduction in glucose consumption. Immunolocalization showed that SjILP is located to lateral gland cells of mature intra-ovular miracidia in the schistosome egg, and is distributed on the ciliated epithelium and internal cell masses of newly transformed miracidia. In schistosomula, SjILP is present on the tegument in two antero-lateral points, indicating highly polarized expression during cercarial transformation. Analysis of serum from S. japonicum-infected mice by ELISA using a recombinant form of SjILP as an antigen revealed IgG immunoreactivity to this molecule at 7 weeks post-infection indicating it is likely secreted from mature eggs into the host circulation. These findings provide further insights on ILP function in schistosomes and its essential roles in parasite survival and growth in different development stages.
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spelling pubmed-64801002019-04-29 Gene Expression in Developmental Stages of Schistosoma japonicum Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide Du, Xiaofeng Jones, Malcolm K. Nawaratna, Sujeevi S. K. Ranasinghe, Shiwanthi Xiong, Chunrong Cai, Pengfei McManus, Donald P. You, Hong Int J Mol Sci Article We showed previously that the Schistosoma japonicum insulin-like peptide (SjILP) binds the worm insulin receptors, thereby, activating the parasite’s insulin pathway and emphasizing its important role in regulating uptake of glucose, a nutrient essential for parasite survival. Here we show that SjILP is differentially expressed in the schistosome life cycle and is especially highly transcribed in eggs, miracidia, and adult female worms. RNA inference was employed to knockdown SjILP in adults in vitro, with suppression confirmed by significantly reduced protein production, declined adenosine diphosphate levels, and reduction in glucose consumption. Immunolocalization showed that SjILP is located to lateral gland cells of mature intra-ovular miracidia in the schistosome egg, and is distributed on the ciliated epithelium and internal cell masses of newly transformed miracidia. In schistosomula, SjILP is present on the tegument in two antero-lateral points, indicating highly polarized expression during cercarial transformation. Analysis of serum from S. japonicum-infected mice by ELISA using a recombinant form of SjILP as an antigen revealed IgG immunoreactivity to this molecule at 7 weeks post-infection indicating it is likely secreted from mature eggs into the host circulation. These findings provide further insights on ILP function in schistosomes and its essential roles in parasite survival and growth in different development stages. MDPI 2019-03-28 /pmc/articles/PMC6480100/ /pubmed/30925781 http://dx.doi.org/10.3390/ijms20071565 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Du, Xiaofeng
Jones, Malcolm K.
Nawaratna, Sujeevi S. K.
Ranasinghe, Shiwanthi
Xiong, Chunrong
Cai, Pengfei
McManus, Donald P.
You, Hong
Gene Expression in Developmental Stages of Schistosoma japonicum Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide
title Gene Expression in Developmental Stages of Schistosoma japonicum Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide
title_full Gene Expression in Developmental Stages of Schistosoma japonicum Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide
title_fullStr Gene Expression in Developmental Stages of Schistosoma japonicum Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide
title_full_unstemmed Gene Expression in Developmental Stages of Schistosoma japonicum Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide
title_short Gene Expression in Developmental Stages of Schistosoma japonicum Provides Further Insight into the Importance of the Schistosome Insulin-Like Peptide
title_sort gene expression in developmental stages of schistosoma japonicum provides further insight into the importance of the schistosome insulin-like peptide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480100/
https://www.ncbi.nlm.nih.gov/pubmed/30925781
http://dx.doi.org/10.3390/ijms20071565
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