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Evaluation of the Biocompatibility of CS-Graphene Oxide Compounds In Vivo

In the last few years, graphene oxide (GO) has gained considerable importance in scaffold preparation for tissue engineering due to the presence of functional groups that allow the interaction between the extracellular matrix and the components of the cellular membrane. The interaction between GO an...

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Autores principales: López Tenorio, Diego, Valencia, Carlos H., Valencia, Cesar, Zuluaga, Fabio, Valencia, Mayra E., Mina, José H., Grande Tovar, Carlos David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480102/
https://www.ncbi.nlm.nih.gov/pubmed/30934823
http://dx.doi.org/10.3390/ijms20071572
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author López Tenorio, Diego
Valencia, Carlos H.
Valencia, Cesar
Zuluaga, Fabio
Valencia, Mayra E.
Mina, José H.
Grande Tovar, Carlos David
author_facet López Tenorio, Diego
Valencia, Carlos H.
Valencia, Cesar
Zuluaga, Fabio
Valencia, Mayra E.
Mina, José H.
Grande Tovar, Carlos David
author_sort López Tenorio, Diego
collection PubMed
description In the last few years, graphene oxide (GO) has gained considerable importance in scaffold preparation for tissue engineering due to the presence of functional groups that allow the interaction between the extracellular matrix and the components of the cellular membrane. The interaction between GO and chitosan (CS) can not only improve the biomechanical properties of the scaffold but also generate a synergistic effect, facilitating tissue recovery. In vivo studies on GO are scarce; therefore, biocompatibility tests on CS-GO scaffolds and bone regeneration experiments on critical size defects were carried out on Wistar rats. Scaffolds made of CS, CS-GO 0.5%, and CS-GO 1% were prepared and implanted on Wistar rats cranial bones for three months. Scaffold samples were analyzed through histochemistry and scanning electron microscopy. The analysis performed showed reabsorption of the material by phagocytic activity and new bone formation. The CS-GO 0.5% formulation gave the best performance in bone regeneration, with excellent biocompatibility. These results show the potential of this compound for tissue regeneration opening and medical applications.
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spelling pubmed-64801022019-04-29 Evaluation of the Biocompatibility of CS-Graphene Oxide Compounds In Vivo López Tenorio, Diego Valencia, Carlos H. Valencia, Cesar Zuluaga, Fabio Valencia, Mayra E. Mina, José H. Grande Tovar, Carlos David Int J Mol Sci Article In the last few years, graphene oxide (GO) has gained considerable importance in scaffold preparation for tissue engineering due to the presence of functional groups that allow the interaction between the extracellular matrix and the components of the cellular membrane. The interaction between GO and chitosan (CS) can not only improve the biomechanical properties of the scaffold but also generate a synergistic effect, facilitating tissue recovery. In vivo studies on GO are scarce; therefore, biocompatibility tests on CS-GO scaffolds and bone regeneration experiments on critical size defects were carried out on Wistar rats. Scaffolds made of CS, CS-GO 0.5%, and CS-GO 1% were prepared and implanted on Wistar rats cranial bones for three months. Scaffold samples were analyzed through histochemistry and scanning electron microscopy. The analysis performed showed reabsorption of the material by phagocytic activity and new bone formation. The CS-GO 0.5% formulation gave the best performance in bone regeneration, with excellent biocompatibility. These results show the potential of this compound for tissue regeneration opening and medical applications. MDPI 2019-03-29 /pmc/articles/PMC6480102/ /pubmed/30934823 http://dx.doi.org/10.3390/ijms20071572 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
López Tenorio, Diego
Valencia, Carlos H.
Valencia, Cesar
Zuluaga, Fabio
Valencia, Mayra E.
Mina, José H.
Grande Tovar, Carlos David
Evaluation of the Biocompatibility of CS-Graphene Oxide Compounds In Vivo
title Evaluation of the Biocompatibility of CS-Graphene Oxide Compounds In Vivo
title_full Evaluation of the Biocompatibility of CS-Graphene Oxide Compounds In Vivo
title_fullStr Evaluation of the Biocompatibility of CS-Graphene Oxide Compounds In Vivo
title_full_unstemmed Evaluation of the Biocompatibility of CS-Graphene Oxide Compounds In Vivo
title_short Evaluation of the Biocompatibility of CS-Graphene Oxide Compounds In Vivo
title_sort evaluation of the biocompatibility of cs-graphene oxide compounds in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480102/
https://www.ncbi.nlm.nih.gov/pubmed/30934823
http://dx.doi.org/10.3390/ijms20071572
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