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Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles

Chitin was extracted from local snow crab shell waste and used as a raw material in the fabrication of porous spherical microgels. The chitin microgels were obtained using a batch process of emulsification and, afterward, gelation. The effects of chitin concentrations, oil and water phase ratios (O:...

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Detalles Bibliográficos
Autores principales: Taokaew, Siriporn, Ofuchi, Mitsumasa, Kobayashi, Takaomi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480158/
https://www.ncbi.nlm.nih.gov/pubmed/30974746
http://dx.doi.org/10.3390/ma12071160
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author Taokaew, Siriporn
Ofuchi, Mitsumasa
Kobayashi, Takaomi
author_facet Taokaew, Siriporn
Ofuchi, Mitsumasa
Kobayashi, Takaomi
author_sort Taokaew, Siriporn
collection PubMed
description Chitin was extracted from local snow crab shell waste and used as a raw material in the fabrication of porous spherical microgels. The chitin microgels were obtained using a batch process of emulsification and, afterward, gelation. The effects of chitin concentrations, oil and water phase ratios (O:W), surfactants, and gelation on the size distribution and morphology of the microgels were investigated. The extracted chitin possessed α-chitin with a degree of acetylation of ~60% and crystallinity of 70%, as confirmed by Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Powder Diffraction (XRD). In the reverse-micellar emulsification, different chitin concentrations in NaOH solution were used as aqueous phases, and n-hexane media containing Span 80-based surfactants were used as dispersion phases. Various HCl solutions were used as gelling agents. Microgels with sizes ranging from ~5–200 μm were obtained relying on these studied parameters. Under the condition of 3% w/w chitin solution using O:W of 15:1 at 5% w/w of Span 80 (hydrophilic-lipophilic balance; HLB of 4.3), the gelation in the emulsified reverse micelles was better controlled and capable of forming spherical microgel particles with a size of 7.1 ± 0.3 μm, when 800 μL of 1 M HCl was added. The prepared chitin microgel exhibited macro-pore morphology and swelling behavior sensitive to the acidic pH.
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spelling pubmed-64801582019-04-29 Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles Taokaew, Siriporn Ofuchi, Mitsumasa Kobayashi, Takaomi Materials (Basel) Article Chitin was extracted from local snow crab shell waste and used as a raw material in the fabrication of porous spherical microgels. The chitin microgels were obtained using a batch process of emulsification and, afterward, gelation. The effects of chitin concentrations, oil and water phase ratios (O:W), surfactants, and gelation on the size distribution and morphology of the microgels were investigated. The extracted chitin possessed α-chitin with a degree of acetylation of ~60% and crystallinity of 70%, as confirmed by Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Powder Diffraction (XRD). In the reverse-micellar emulsification, different chitin concentrations in NaOH solution were used as aqueous phases, and n-hexane media containing Span 80-based surfactants were used as dispersion phases. Various HCl solutions were used as gelling agents. Microgels with sizes ranging from ~5–200 μm were obtained relying on these studied parameters. Under the condition of 3% w/w chitin solution using O:W of 15:1 at 5% w/w of Span 80 (hydrophilic-lipophilic balance; HLB of 4.3), the gelation in the emulsified reverse micelles was better controlled and capable of forming spherical microgel particles with a size of 7.1 ± 0.3 μm, when 800 μL of 1 M HCl was added. The prepared chitin microgel exhibited macro-pore morphology and swelling behavior sensitive to the acidic pH. MDPI 2019-04-10 /pmc/articles/PMC6480158/ /pubmed/30974746 http://dx.doi.org/10.3390/ma12071160 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Taokaew, Siriporn
Ofuchi, Mitsumasa
Kobayashi, Takaomi
Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles
title Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles
title_full Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles
title_fullStr Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles
title_full_unstemmed Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles
title_short Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles
title_sort size distribution and characteristics of chitin microgels prepared via emulsified reverse-micelles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480158/
https://www.ncbi.nlm.nih.gov/pubmed/30974746
http://dx.doi.org/10.3390/ma12071160
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