Cargando…
Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles
Chitin was extracted from local snow crab shell waste and used as a raw material in the fabrication of porous spherical microgels. The chitin microgels were obtained using a batch process of emulsification and, afterward, gelation. The effects of chitin concentrations, oil and water phase ratios (O:...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480158/ https://www.ncbi.nlm.nih.gov/pubmed/30974746 http://dx.doi.org/10.3390/ma12071160 |
_version_ | 1783413511843479552 |
---|---|
author | Taokaew, Siriporn Ofuchi, Mitsumasa Kobayashi, Takaomi |
author_facet | Taokaew, Siriporn Ofuchi, Mitsumasa Kobayashi, Takaomi |
author_sort | Taokaew, Siriporn |
collection | PubMed |
description | Chitin was extracted from local snow crab shell waste and used as a raw material in the fabrication of porous spherical microgels. The chitin microgels were obtained using a batch process of emulsification and, afterward, gelation. The effects of chitin concentrations, oil and water phase ratios (O:W), surfactants, and gelation on the size distribution and morphology of the microgels were investigated. The extracted chitin possessed α-chitin with a degree of acetylation of ~60% and crystallinity of 70%, as confirmed by Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Powder Diffraction (XRD). In the reverse-micellar emulsification, different chitin concentrations in NaOH solution were used as aqueous phases, and n-hexane media containing Span 80-based surfactants were used as dispersion phases. Various HCl solutions were used as gelling agents. Microgels with sizes ranging from ~5–200 μm were obtained relying on these studied parameters. Under the condition of 3% w/w chitin solution using O:W of 15:1 at 5% w/w of Span 80 (hydrophilic-lipophilic balance; HLB of 4.3), the gelation in the emulsified reverse micelles was better controlled and capable of forming spherical microgel particles with a size of 7.1 ± 0.3 μm, when 800 μL of 1 M HCl was added. The prepared chitin microgel exhibited macro-pore morphology and swelling behavior sensitive to the acidic pH. |
format | Online Article Text |
id | pubmed-6480158 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64801582019-04-29 Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles Taokaew, Siriporn Ofuchi, Mitsumasa Kobayashi, Takaomi Materials (Basel) Article Chitin was extracted from local snow crab shell waste and used as a raw material in the fabrication of porous spherical microgels. The chitin microgels were obtained using a batch process of emulsification and, afterward, gelation. The effects of chitin concentrations, oil and water phase ratios (O:W), surfactants, and gelation on the size distribution and morphology of the microgels were investigated. The extracted chitin possessed α-chitin with a degree of acetylation of ~60% and crystallinity of 70%, as confirmed by Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Powder Diffraction (XRD). In the reverse-micellar emulsification, different chitin concentrations in NaOH solution were used as aqueous phases, and n-hexane media containing Span 80-based surfactants were used as dispersion phases. Various HCl solutions were used as gelling agents. Microgels with sizes ranging from ~5–200 μm were obtained relying on these studied parameters. Under the condition of 3% w/w chitin solution using O:W of 15:1 at 5% w/w of Span 80 (hydrophilic-lipophilic balance; HLB of 4.3), the gelation in the emulsified reverse micelles was better controlled and capable of forming spherical microgel particles with a size of 7.1 ± 0.3 μm, when 800 μL of 1 M HCl was added. The prepared chitin microgel exhibited macro-pore morphology and swelling behavior sensitive to the acidic pH. MDPI 2019-04-10 /pmc/articles/PMC6480158/ /pubmed/30974746 http://dx.doi.org/10.3390/ma12071160 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Taokaew, Siriporn Ofuchi, Mitsumasa Kobayashi, Takaomi Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles |
title | Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles |
title_full | Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles |
title_fullStr | Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles |
title_full_unstemmed | Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles |
title_short | Size Distribution and Characteristics of Chitin Microgels Prepared via Emulsified Reverse-Micelles |
title_sort | size distribution and characteristics of chitin microgels prepared via emulsified reverse-micelles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480158/ https://www.ncbi.nlm.nih.gov/pubmed/30974746 http://dx.doi.org/10.3390/ma12071160 |
work_keys_str_mv | AT taokaewsiriporn sizedistributionandcharacteristicsofchitinmicrogelspreparedviaemulsifiedreversemicelles AT ofuchimitsumasa sizedistributionandcharacteristicsofchitinmicrogelspreparedviaemulsifiedreversemicelles AT kobayashitakaomi sizedistributionandcharacteristicsofchitinmicrogelspreparedviaemulsifiedreversemicelles |