Cargando…
Comparative Transcriptome Analysis of Ampelopsis megalophylla for Identifying Genes Involved in Flavonoid Biosynthesis and Accumulation during Different Seasons
Ampelopsis megalophylla is an important species used in Chinese folk medicine. Flavonoids, the most important active components of plants, greatly determine the quality of A. megalophylla. However, biosynthesis of flavonoids at the molecular and genetic levels in A. megalophylla is not well understo...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480179/ https://www.ncbi.nlm.nih.gov/pubmed/30939828 http://dx.doi.org/10.3390/molecules24071267 |
_version_ | 1783413516648054784 |
---|---|
author | Yang, Min Zhou, Peina Gui, Chun Da, Guozheng Gong, Ling Zhang, Xiuqiao |
author_facet | Yang, Min Zhou, Peina Gui, Chun Da, Guozheng Gong, Ling Zhang, Xiuqiao |
author_sort | Yang, Min |
collection | PubMed |
description | Ampelopsis megalophylla is an important species used in Chinese folk medicine. Flavonoids, the most important active components of plants, greatly determine the quality of A. megalophylla. However, biosynthesis of flavonoids at the molecular and genetic levels in A. megalophylla is not well understood. In this study, we performed chemical analysis and transcriptome analysis of A. megalophylla in different seasons (i.e., May, August, and October). Accumulation of flavonoids was higher in May than in the other two months. Genes involved in the flavonoid biosynthesis pathway, such as chalcone synthase, anthocyanidin synthase, flavanone 3-hydroxylase, flavonoid-3′,5′-hydroxylase, caffeoyl-CoA O-methyltransferase, dihydroflavonol 4-reductase, 4-coumarate-CoA ligase, phenylalanine ammonia-lyase, cinnamate 4-hydroxylase, flavonoid 3′-monooxygenase, shikimate O-hydroxycinnamoyltransferase, and leucoanthocyanidin reductase, were identified based on transcriptome data. Fifty ATP binding cassette (ABC) transporter, nine SNARE, forty-nine GST, and eighty-four glycosyltransferases unigenes related to flavonoid transport and biomodification were also found. Moreover, seventy-eight cytochrome P450s and multiple transcription factors (five MYB, two bHLH, and three WD40 family genes) may be associated with the regulation of the flavonoid biosynthesis process. These results provide insights into the molecular processes of flavonoid biosynthesis in A. megalophylla and offer a significant resource for the application of genetic engineering in developing varieties with improved quality. |
format | Online Article Text |
id | pubmed-6480179 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64801792019-04-30 Comparative Transcriptome Analysis of Ampelopsis megalophylla for Identifying Genes Involved in Flavonoid Biosynthesis and Accumulation during Different Seasons Yang, Min Zhou, Peina Gui, Chun Da, Guozheng Gong, Ling Zhang, Xiuqiao Molecules Article Ampelopsis megalophylla is an important species used in Chinese folk medicine. Flavonoids, the most important active components of plants, greatly determine the quality of A. megalophylla. However, biosynthesis of flavonoids at the molecular and genetic levels in A. megalophylla is not well understood. In this study, we performed chemical analysis and transcriptome analysis of A. megalophylla in different seasons (i.e., May, August, and October). Accumulation of flavonoids was higher in May than in the other two months. Genes involved in the flavonoid biosynthesis pathway, such as chalcone synthase, anthocyanidin synthase, flavanone 3-hydroxylase, flavonoid-3′,5′-hydroxylase, caffeoyl-CoA O-methyltransferase, dihydroflavonol 4-reductase, 4-coumarate-CoA ligase, phenylalanine ammonia-lyase, cinnamate 4-hydroxylase, flavonoid 3′-monooxygenase, shikimate O-hydroxycinnamoyltransferase, and leucoanthocyanidin reductase, were identified based on transcriptome data. Fifty ATP binding cassette (ABC) transporter, nine SNARE, forty-nine GST, and eighty-four glycosyltransferases unigenes related to flavonoid transport and biomodification were also found. Moreover, seventy-eight cytochrome P450s and multiple transcription factors (five MYB, two bHLH, and three WD40 family genes) may be associated with the regulation of the flavonoid biosynthesis process. These results provide insights into the molecular processes of flavonoid biosynthesis in A. megalophylla and offer a significant resource for the application of genetic engineering in developing varieties with improved quality. MDPI 2019-04-01 /pmc/articles/PMC6480179/ /pubmed/30939828 http://dx.doi.org/10.3390/molecules24071267 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Min Zhou, Peina Gui, Chun Da, Guozheng Gong, Ling Zhang, Xiuqiao Comparative Transcriptome Analysis of Ampelopsis megalophylla for Identifying Genes Involved in Flavonoid Biosynthesis and Accumulation during Different Seasons |
title | Comparative Transcriptome Analysis of Ampelopsis megalophylla for Identifying Genes Involved in Flavonoid Biosynthesis and Accumulation during Different Seasons |
title_full | Comparative Transcriptome Analysis of Ampelopsis megalophylla for Identifying Genes Involved in Flavonoid Biosynthesis and Accumulation during Different Seasons |
title_fullStr | Comparative Transcriptome Analysis of Ampelopsis megalophylla for Identifying Genes Involved in Flavonoid Biosynthesis and Accumulation during Different Seasons |
title_full_unstemmed | Comparative Transcriptome Analysis of Ampelopsis megalophylla for Identifying Genes Involved in Flavonoid Biosynthesis and Accumulation during Different Seasons |
title_short | Comparative Transcriptome Analysis of Ampelopsis megalophylla for Identifying Genes Involved in Flavonoid Biosynthesis and Accumulation during Different Seasons |
title_sort | comparative transcriptome analysis of ampelopsis megalophylla for identifying genes involved in flavonoid biosynthesis and accumulation during different seasons |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480179/ https://www.ncbi.nlm.nih.gov/pubmed/30939828 http://dx.doi.org/10.3390/molecules24071267 |
work_keys_str_mv | AT yangmin comparativetranscriptomeanalysisofampelopsismegalophyllaforidentifyinggenesinvolvedinflavonoidbiosynthesisandaccumulationduringdifferentseasons AT zhoupeina comparativetranscriptomeanalysisofampelopsismegalophyllaforidentifyinggenesinvolvedinflavonoidbiosynthesisandaccumulationduringdifferentseasons AT guichun comparativetranscriptomeanalysisofampelopsismegalophyllaforidentifyinggenesinvolvedinflavonoidbiosynthesisandaccumulationduringdifferentseasons AT daguozheng comparativetranscriptomeanalysisofampelopsismegalophyllaforidentifyinggenesinvolvedinflavonoidbiosynthesisandaccumulationduringdifferentseasons AT gongling comparativetranscriptomeanalysisofampelopsismegalophyllaforidentifyinggenesinvolvedinflavonoidbiosynthesisandaccumulationduringdifferentseasons AT zhangxiuqiao comparativetranscriptomeanalysisofampelopsismegalophyllaforidentifyinggenesinvolvedinflavonoidbiosynthesisandaccumulationduringdifferentseasons |