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The Role of Tyramine β-Hydroxylase in Density Dependent Immunityof Oriental Armyworm (Mythmina separata) Larva

High population density alters insect prophylactic immunity, with density-dependent prophylaxis (DDP) being reported in many polyphonic insects. However, the molecular mechanism for DDP remains unclear. In current study, the role of tyramine β-hydroxylase (Tβh) in the immune response of M. separata...

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Autores principales: Kong, Hailong, Dong, Chuanlei, Jing, Wanghui, Tian, Zhen, Zheng, Minyuan, Wang, Cheng, Hou, Qiuli, Cheng, Yunxia, Zhang, Lei, Jiang, Xingfu, Luo, Lizhi, Zhu, Shude
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480284/
https://www.ncbi.nlm.nih.gov/pubmed/30925699
http://dx.doi.org/10.3390/ijms20071553
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author Kong, Hailong
Dong, Chuanlei
Jing, Wanghui
Tian, Zhen
Zheng, Minyuan
Wang, Cheng
Hou, Qiuli
Cheng, Yunxia
Zhang, Lei
Jiang, Xingfu
Luo, Lizhi
Zhu, Shude
author_facet Kong, Hailong
Dong, Chuanlei
Jing, Wanghui
Tian, Zhen
Zheng, Minyuan
Wang, Cheng
Hou, Qiuli
Cheng, Yunxia
Zhang, Lei
Jiang, Xingfu
Luo, Lizhi
Zhu, Shude
author_sort Kong, Hailong
collection PubMed
description High population density alters insect prophylactic immunity, with density-dependent prophylaxis (DDP) being reported in many polyphonic insects. However, the molecular mechanism for DDP remains unclear. In current study, the role of tyramine β-hydroxylase (Tβh) in the immune response of M. separata larvae that were subject to different rearing densities conditions was investigated. The tyramine β-hydroxylase activity of larvae from high density treatments (10 and 30 larvae per jar) was significantly higher than that of the larvae from low density treatments (one, two, and five larvae/jar). A tyramine β-hydroxylase (designated MsTβh) containing a 1779 bp open reading frame was identified. Multiple sequence alignment and phylogenetic analysis indicated that MsTβh was orthologous to the Tβh that was found in other lepidopterans. Elevated MsTβh expression was observed in larvae under high density (10 larvae per jar). Silencing MsTβh expression by the injection of dsRNA in larvae from the high density treatment produced a 25.1% reduction in octopamine levels, while at the same time, there was a significant decrease in phenoloxidase (PO) and lysozyme activity, total haemocyte counts, and survival against Beauveria infection 56.6%, 88.5%, 82.0%, and 55.8%, respectively, when compared to control larvae. Our findings provide the first insights into how MsTβh mediates the octopamine level, which in turn modulates the immune response of larvae under different population densities.
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spelling pubmed-64802842019-04-29 The Role of Tyramine β-Hydroxylase in Density Dependent Immunityof Oriental Armyworm (Mythmina separata) Larva Kong, Hailong Dong, Chuanlei Jing, Wanghui Tian, Zhen Zheng, Minyuan Wang, Cheng Hou, Qiuli Cheng, Yunxia Zhang, Lei Jiang, Xingfu Luo, Lizhi Zhu, Shude Int J Mol Sci Article High population density alters insect prophylactic immunity, with density-dependent prophylaxis (DDP) being reported in many polyphonic insects. However, the molecular mechanism for DDP remains unclear. In current study, the role of tyramine β-hydroxylase (Tβh) in the immune response of M. separata larvae that were subject to different rearing densities conditions was investigated. The tyramine β-hydroxylase activity of larvae from high density treatments (10 and 30 larvae per jar) was significantly higher than that of the larvae from low density treatments (one, two, and five larvae/jar). A tyramine β-hydroxylase (designated MsTβh) containing a 1779 bp open reading frame was identified. Multiple sequence alignment and phylogenetic analysis indicated that MsTβh was orthologous to the Tβh that was found in other lepidopterans. Elevated MsTβh expression was observed in larvae under high density (10 larvae per jar). Silencing MsTβh expression by the injection of dsRNA in larvae from the high density treatment produced a 25.1% reduction in octopamine levels, while at the same time, there was a significant decrease in phenoloxidase (PO) and lysozyme activity, total haemocyte counts, and survival against Beauveria infection 56.6%, 88.5%, 82.0%, and 55.8%, respectively, when compared to control larvae. Our findings provide the first insights into how MsTβh mediates the octopamine level, which in turn modulates the immune response of larvae under different population densities. MDPI 2019-03-28 /pmc/articles/PMC6480284/ /pubmed/30925699 http://dx.doi.org/10.3390/ijms20071553 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kong, Hailong
Dong, Chuanlei
Jing, Wanghui
Tian, Zhen
Zheng, Minyuan
Wang, Cheng
Hou, Qiuli
Cheng, Yunxia
Zhang, Lei
Jiang, Xingfu
Luo, Lizhi
Zhu, Shude
The Role of Tyramine β-Hydroxylase in Density Dependent Immunityof Oriental Armyworm (Mythmina separata) Larva
title The Role of Tyramine β-Hydroxylase in Density Dependent Immunityof Oriental Armyworm (Mythmina separata) Larva
title_full The Role of Tyramine β-Hydroxylase in Density Dependent Immunityof Oriental Armyworm (Mythmina separata) Larva
title_fullStr The Role of Tyramine β-Hydroxylase in Density Dependent Immunityof Oriental Armyworm (Mythmina separata) Larva
title_full_unstemmed The Role of Tyramine β-Hydroxylase in Density Dependent Immunityof Oriental Armyworm (Mythmina separata) Larva
title_short The Role of Tyramine β-Hydroxylase in Density Dependent Immunityof Oriental Armyworm (Mythmina separata) Larva
title_sort role of tyramine β-hydroxylase in density dependent immunityof oriental armyworm (mythmina separata) larva
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480284/
https://www.ncbi.nlm.nih.gov/pubmed/30925699
http://dx.doi.org/10.3390/ijms20071553
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