Cargando…
High Protein Diet Induces Oxidative Stress in Rat Cerebral Cortex and Hypothalamus
This is the first study to analyze the impact of high protein diet (HPD) on antioxidant defense, redox status, as well as oxidative damage on both a local and systemic level. Male Wistar rats were divided into two equal groups (n = 9): HPD (44% protein) and standard diet (CON; 24.2% protein). After...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480352/ https://www.ncbi.nlm.nih.gov/pubmed/30925663 http://dx.doi.org/10.3390/ijms20071547 |
_version_ | 1783413554150375424 |
---|---|
author | Żebrowska, Ewa Maciejczyk, Mateusz Żendzian-Piotrowska, Małgorzata Zalewska, Anna Chabowski, Adrian |
author_facet | Żebrowska, Ewa Maciejczyk, Mateusz Żendzian-Piotrowska, Małgorzata Zalewska, Anna Chabowski, Adrian |
author_sort | Żebrowska, Ewa |
collection | PubMed |
description | This is the first study to analyze the impact of high protein diet (HPD) on antioxidant defense, redox status, as well as oxidative damage on both a local and systemic level. Male Wistar rats were divided into two equal groups (n = 9): HPD (44% protein) and standard diet (CON; 24.2% protein). After eight weeks, glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), superoxide dismutase-1 (SOD-1), reduced glutathione (GSH), uric acid (UA), total antioxidant (TAC)/oxidant status (TOS) as well as advanced glycation end products (AGE), 4-hydroxynonenal (4-HNE), and malondialdehyde (MDA) were analyzed in the serum/plasma, cerebral cortex, and hypothalamus of HPD and CON rats. HPD resulted in higher UA concentration and activity of GPx and CAT in the hypothalamus, whereas in the cerebral cortex these parameters remained unchanged. A significantly lower GSH content was demonstrated in the plasma and hypothalamus of HPD rats when compared to CON rats. Both brain structures expressed higher content of 4-HNE and MDA, whereas AGE was increased only in the hypothalamus of HPD animals. Despite the enhancement in antioxidant defense in the hypothalamus, this mechanism does not protect the hypothalamus from oxidative damage in rats. Hypothalamus is more susceptible to oxidative stress caused by HPD. |
format | Online Article Text |
id | pubmed-6480352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64803522019-04-29 High Protein Diet Induces Oxidative Stress in Rat Cerebral Cortex and Hypothalamus Żebrowska, Ewa Maciejczyk, Mateusz Żendzian-Piotrowska, Małgorzata Zalewska, Anna Chabowski, Adrian Int J Mol Sci Article This is the first study to analyze the impact of high protein diet (HPD) on antioxidant defense, redox status, as well as oxidative damage on both a local and systemic level. Male Wistar rats were divided into two equal groups (n = 9): HPD (44% protein) and standard diet (CON; 24.2% protein). After eight weeks, glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), superoxide dismutase-1 (SOD-1), reduced glutathione (GSH), uric acid (UA), total antioxidant (TAC)/oxidant status (TOS) as well as advanced glycation end products (AGE), 4-hydroxynonenal (4-HNE), and malondialdehyde (MDA) were analyzed in the serum/plasma, cerebral cortex, and hypothalamus of HPD and CON rats. HPD resulted in higher UA concentration and activity of GPx and CAT in the hypothalamus, whereas in the cerebral cortex these parameters remained unchanged. A significantly lower GSH content was demonstrated in the plasma and hypothalamus of HPD rats when compared to CON rats. Both brain structures expressed higher content of 4-HNE and MDA, whereas AGE was increased only in the hypothalamus of HPD animals. Despite the enhancement in antioxidant defense in the hypothalamus, this mechanism does not protect the hypothalamus from oxidative damage in rats. Hypothalamus is more susceptible to oxidative stress caused by HPD. MDPI 2019-03-28 /pmc/articles/PMC6480352/ /pubmed/30925663 http://dx.doi.org/10.3390/ijms20071547 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Żebrowska, Ewa Maciejczyk, Mateusz Żendzian-Piotrowska, Małgorzata Zalewska, Anna Chabowski, Adrian High Protein Diet Induces Oxidative Stress in Rat Cerebral Cortex and Hypothalamus |
title | High Protein Diet Induces Oxidative Stress in Rat Cerebral Cortex and Hypothalamus |
title_full | High Protein Diet Induces Oxidative Stress in Rat Cerebral Cortex and Hypothalamus |
title_fullStr | High Protein Diet Induces Oxidative Stress in Rat Cerebral Cortex and Hypothalamus |
title_full_unstemmed | High Protein Diet Induces Oxidative Stress in Rat Cerebral Cortex and Hypothalamus |
title_short | High Protein Diet Induces Oxidative Stress in Rat Cerebral Cortex and Hypothalamus |
title_sort | high protein diet induces oxidative stress in rat cerebral cortex and hypothalamus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480352/ https://www.ncbi.nlm.nih.gov/pubmed/30925663 http://dx.doi.org/10.3390/ijms20071547 |
work_keys_str_mv | AT zebrowskaewa highproteindietinducesoxidativestressinratcerebralcortexandhypothalamus AT maciejczykmateusz highproteindietinducesoxidativestressinratcerebralcortexandhypothalamus AT zendzianpiotrowskamałgorzata highproteindietinducesoxidativestressinratcerebralcortexandhypothalamus AT zalewskaanna highproteindietinducesoxidativestressinratcerebralcortexandhypothalamus AT chabowskiadrian highproteindietinducesoxidativestressinratcerebralcortexandhypothalamus |