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Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays
Background: Galium is a plant rich in iridoid glycosides, flavonoids, anthraquinones, and small amounts of essential oils and vitamin C. Recent works showed the antibacterial, antifungal, antiparasitic, and antioxidant activity of this plant genus. Methods: For the determination of the multicomponen...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480365/ https://www.ncbi.nlm.nih.gov/pubmed/30925810 http://dx.doi.org/10.3390/molecules24071226 |
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author | Mocan, Andrei Diuzheva, Alina Bădărău, Sabin Moldovan, Cadmiel Andruch, Vasil Carradori, Simone Campestre, Cristina Tartaglia, Angela De Simone, Marta Vodnar, Dan Tiecco, Matteo Germani, Raimondo Crișan, Gianina Locatelli, Marcello |
author_facet | Mocan, Andrei Diuzheva, Alina Bădărău, Sabin Moldovan, Cadmiel Andruch, Vasil Carradori, Simone Campestre, Cristina Tartaglia, Angela De Simone, Marta Vodnar, Dan Tiecco, Matteo Germani, Raimondo Crișan, Gianina Locatelli, Marcello |
author_sort | Mocan, Andrei |
collection | PubMed |
description | Background: Galium is a plant rich in iridoid glycosides, flavonoids, anthraquinones, and small amounts of essential oils and vitamin C. Recent works showed the antibacterial, antifungal, antiparasitic, and antioxidant activity of this plant genus. Methods: For the determination of the multicomponent phenolic pattern, liquid phase microextraction procedures were applied, combined with HPLC-PDA instrument configuration in five Galium species aerial parts (G. verum, G. album, G. rivale, G. pseudoaristatum, and G. purpureum). Dispersive Liquid–Liquid MicroExtraction (DLLME) with NaCl and NAtural Deep Eutectic Solvent (NADES) medium and Ultrasound-Assisted (UA)-DLLME with β-cyclodextrin medium were optimized. Results: The optimal DLLME conditions were found to be: 10 mg of the sample, 10% NaCl, 15% NADES or 1% β-cyclodextrin as extraction solvent—400 μL of ethyl acetate as dispersive solvent—300 μL of ethanol, vortex time—30 s, extraction time—1 min, centrifugation at 12000× g for 5 min. Conclusions: These results were compared with microwave-assisted extraction procedures. G. purpureum and G. verum extracts showed the highest total phenolic and flavonoid content, respectively. The most potent extract in terms of antioxidant capacity was obtained from G. purpureum, whereas the extract obtained from G. album exhibited the strongest inhibitory effect against tyrosinase. |
format | Online Article Text |
id | pubmed-6480365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64803652019-04-30 Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays Mocan, Andrei Diuzheva, Alina Bădărău, Sabin Moldovan, Cadmiel Andruch, Vasil Carradori, Simone Campestre, Cristina Tartaglia, Angela De Simone, Marta Vodnar, Dan Tiecco, Matteo Germani, Raimondo Crișan, Gianina Locatelli, Marcello Molecules Article Background: Galium is a plant rich in iridoid glycosides, flavonoids, anthraquinones, and small amounts of essential oils and vitamin C. Recent works showed the antibacterial, antifungal, antiparasitic, and antioxidant activity of this plant genus. Methods: For the determination of the multicomponent phenolic pattern, liquid phase microextraction procedures were applied, combined with HPLC-PDA instrument configuration in five Galium species aerial parts (G. verum, G. album, G. rivale, G. pseudoaristatum, and G. purpureum). Dispersive Liquid–Liquid MicroExtraction (DLLME) with NaCl and NAtural Deep Eutectic Solvent (NADES) medium and Ultrasound-Assisted (UA)-DLLME with β-cyclodextrin medium were optimized. Results: The optimal DLLME conditions were found to be: 10 mg of the sample, 10% NaCl, 15% NADES or 1% β-cyclodextrin as extraction solvent—400 μL of ethyl acetate as dispersive solvent—300 μL of ethanol, vortex time—30 s, extraction time—1 min, centrifugation at 12000× g for 5 min. Conclusions: These results were compared with microwave-assisted extraction procedures. G. purpureum and G. verum extracts showed the highest total phenolic and flavonoid content, respectively. The most potent extract in terms of antioxidant capacity was obtained from G. purpureum, whereas the extract obtained from G. album exhibited the strongest inhibitory effect against tyrosinase. MDPI 2019-03-28 /pmc/articles/PMC6480365/ /pubmed/30925810 http://dx.doi.org/10.3390/molecules24071226 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mocan, Andrei Diuzheva, Alina Bădărău, Sabin Moldovan, Cadmiel Andruch, Vasil Carradori, Simone Campestre, Cristina Tartaglia, Angela De Simone, Marta Vodnar, Dan Tiecco, Matteo Germani, Raimondo Crișan, Gianina Locatelli, Marcello Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays |
title | Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays |
title_full | Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays |
title_fullStr | Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays |
title_full_unstemmed | Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays |
title_short | Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays |
title_sort | liquid phase and microwave-assisted extractions for multicomponent phenolic pattern determination of five romanian galium species coupled with bioassays |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480365/ https://www.ncbi.nlm.nih.gov/pubmed/30925810 http://dx.doi.org/10.3390/molecules24071226 |
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