Cargando…

Molecular Cloning, Characterization, and Nutritional Regulation of Elovl6 in Large Yellow Croaker (Larimichthys crocea)

Elongation of very long chain fatty acids protein 6 (Elovl6) is a key enzyme in fatty acid synthesis, which participates in converting palmitate (C16:0) to stearate (C18:0). Although studies of Elovl6 have been carried out in mammals, the nutritional regulation of elovl6 in fish remains poorly under...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Yongnan, Pang, Yuning, Xiang, Xiaojun, Du, Jianlong, Mai, Kangsen, Ai, Qinghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480403/
https://www.ncbi.nlm.nih.gov/pubmed/30979053
http://dx.doi.org/10.3390/ijms20071801
_version_ 1783413563570782208
author Li, Yongnan
Pang, Yuning
Xiang, Xiaojun
Du, Jianlong
Mai, Kangsen
Ai, Qinghui
author_facet Li, Yongnan
Pang, Yuning
Xiang, Xiaojun
Du, Jianlong
Mai, Kangsen
Ai, Qinghui
author_sort Li, Yongnan
collection PubMed
description Elongation of very long chain fatty acids protein 6 (Elovl6) is a key enzyme in fatty acid synthesis, which participates in converting palmitate (C16:0) to stearate (C18:0). Although studies of Elovl6 have been carried out in mammals, the nutritional regulation of elovl6 in fish remains poorly understood. In the present study, the cloning and nutritional regulation of elovl6 were determined in large yellow croaker. Sequence and phylogenetic analysis revealed that the full-length cDNA of elovl6 was 1360 bp, including an open reading frame of 810 bp encoding a putative protein of 269 amino acid that possesses the characteristic features of Elovl proteins. The transcript level of elovl6 was significantly increased in the liver of croaker fed the diets with soybean oil (enriched with 18: 2n-6, LA) or linseed oil (enriched with 18: 3n-3, ALA) than that in croaker fed the diet with fish oil (enriched with 20: 5n-3 and 22: 6n-3). Correspondingly, the elovl6 expression in croaker’s hepatocytes treated with ALA or LA was remarkably increased compared to the controls. Furthermore, the transcription factors including hepatocyte nuclear factor 1α (HNF1α), CCAAT-enhancer-binding protein β (CEBPβ), retinoid X receptor α (RXRα), and cAMP response element-binding protein 1 (CREB1) greatly enhanced promoter activity of elovl6 in large yellow croaker, and the expression of transcription factors is consistent with the changes of elovl6 expression in response to fatty acids in vivo and in vitro. In conclusion, this study revealed that elovl6 expression in large yellow croaker could be upregulated by dietary ALA or LA via the increased transcriptional expression of transcription factors including hnf1α, cebpβ, rxrα, and creb1.
format Online
Article
Text
id pubmed-6480403
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64804032019-04-29 Molecular Cloning, Characterization, and Nutritional Regulation of Elovl6 in Large Yellow Croaker (Larimichthys crocea) Li, Yongnan Pang, Yuning Xiang, Xiaojun Du, Jianlong Mai, Kangsen Ai, Qinghui Int J Mol Sci Article Elongation of very long chain fatty acids protein 6 (Elovl6) is a key enzyme in fatty acid synthesis, which participates in converting palmitate (C16:0) to stearate (C18:0). Although studies of Elovl6 have been carried out in mammals, the nutritional regulation of elovl6 in fish remains poorly understood. In the present study, the cloning and nutritional regulation of elovl6 were determined in large yellow croaker. Sequence and phylogenetic analysis revealed that the full-length cDNA of elovl6 was 1360 bp, including an open reading frame of 810 bp encoding a putative protein of 269 amino acid that possesses the characteristic features of Elovl proteins. The transcript level of elovl6 was significantly increased in the liver of croaker fed the diets with soybean oil (enriched with 18: 2n-6, LA) or linseed oil (enriched with 18: 3n-3, ALA) than that in croaker fed the diet with fish oil (enriched with 20: 5n-3 and 22: 6n-3). Correspondingly, the elovl6 expression in croaker’s hepatocytes treated with ALA or LA was remarkably increased compared to the controls. Furthermore, the transcription factors including hepatocyte nuclear factor 1α (HNF1α), CCAAT-enhancer-binding protein β (CEBPβ), retinoid X receptor α (RXRα), and cAMP response element-binding protein 1 (CREB1) greatly enhanced promoter activity of elovl6 in large yellow croaker, and the expression of transcription factors is consistent with the changes of elovl6 expression in response to fatty acids in vivo and in vitro. In conclusion, this study revealed that elovl6 expression in large yellow croaker could be upregulated by dietary ALA or LA via the increased transcriptional expression of transcription factors including hnf1α, cebpβ, rxrα, and creb1. MDPI 2019-04-11 /pmc/articles/PMC6480403/ /pubmed/30979053 http://dx.doi.org/10.3390/ijms20071801 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Yongnan
Pang, Yuning
Xiang, Xiaojun
Du, Jianlong
Mai, Kangsen
Ai, Qinghui
Molecular Cloning, Characterization, and Nutritional Regulation of Elovl6 in Large Yellow Croaker (Larimichthys crocea)
title Molecular Cloning, Characterization, and Nutritional Regulation of Elovl6 in Large Yellow Croaker (Larimichthys crocea)
title_full Molecular Cloning, Characterization, and Nutritional Regulation of Elovl6 in Large Yellow Croaker (Larimichthys crocea)
title_fullStr Molecular Cloning, Characterization, and Nutritional Regulation of Elovl6 in Large Yellow Croaker (Larimichthys crocea)
title_full_unstemmed Molecular Cloning, Characterization, and Nutritional Regulation of Elovl6 in Large Yellow Croaker (Larimichthys crocea)
title_short Molecular Cloning, Characterization, and Nutritional Regulation of Elovl6 in Large Yellow Croaker (Larimichthys crocea)
title_sort molecular cloning, characterization, and nutritional regulation of elovl6 in large yellow croaker (larimichthys crocea)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480403/
https://www.ncbi.nlm.nih.gov/pubmed/30979053
http://dx.doi.org/10.3390/ijms20071801
work_keys_str_mv AT liyongnan molecularcloningcharacterizationandnutritionalregulationofelovl6inlargeyellowcroakerlarimichthyscrocea
AT pangyuning molecularcloningcharacterizationandnutritionalregulationofelovl6inlargeyellowcroakerlarimichthyscrocea
AT xiangxiaojun molecularcloningcharacterizationandnutritionalregulationofelovl6inlargeyellowcroakerlarimichthyscrocea
AT dujianlong molecularcloningcharacterizationandnutritionalregulationofelovl6inlargeyellowcroakerlarimichthyscrocea
AT maikangsen molecularcloningcharacterizationandnutritionalregulationofelovl6inlargeyellowcroakerlarimichthyscrocea
AT aiqinghui molecularcloningcharacterizationandnutritionalregulationofelovl6inlargeyellowcroakerlarimichthyscrocea