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Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats

Under some occupational conditions, workers are inevitably exposed to high-intensity radiofrequency (RF) fields. In this study, we investigated the effects of one-month exposure to a 220 MHz pulsed modulated RF field at the power density of 50 W/m(2) on the sperm quality in male adult rats. The sper...

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Autores principales: Guo, Ling, Lin, Jia-Jin, Xue, Yi-Zhe, An, Guang-Zhou, Zhang, Jun-Ping, Zhang, Ke-Ying, He, Wei, Wang, Huan, Li, Wei, Ding, Gui-Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480634/
https://www.ncbi.nlm.nih.gov/pubmed/30974849
http://dx.doi.org/10.3390/ijerph16071286
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author Guo, Ling
Lin, Jia-Jin
Xue, Yi-Zhe
An, Guang-Zhou
Zhang, Jun-Ping
Zhang, Ke-Ying
He, Wei
Wang, Huan
Li, Wei
Ding, Gui-Rong
author_facet Guo, Ling
Lin, Jia-Jin
Xue, Yi-Zhe
An, Guang-Zhou
Zhang, Jun-Ping
Zhang, Ke-Ying
He, Wei
Wang, Huan
Li, Wei
Ding, Gui-Rong
author_sort Guo, Ling
collection PubMed
description Under some occupational conditions, workers are inevitably exposed to high-intensity radiofrequency (RF) fields. In this study, we investigated the effects of one-month exposure to a 220 MHz pulsed modulated RF field at the power density of 50 W/m(2) on the sperm quality in male adult rats. The sperm quality was evaluated by measuring the number, abnormality and survival rate of sperm cells. The morphology of testis was examined by hematoxylin–eosin (HE) staining. The levels of secreting factors by Sertoli cells (SCs) and Leydig cells (LCs) were determined by enzyme linked immunosorbent assay (ELISA). The level of cleaved caspase 3 in the testis was detected by immunofluorescence staining. Finally, the expression levels of the apoptosis-related protein (caspase 3, BAX and BCL2) in the testis were assessed by Western blotting. Compared with the sham group, the sperm quality in the RF group decreased significantly. The levels of secreting factors of SCs and the morphology of the testis showed an obvious change after RF exposure. The level of the secreting factor of LCs decreased significantly after RF exposure. The levels of cleaved caspase 3, caspase 3, and the BAX/BCL2 ratio in the testis increased markedly after RF exposure. These data collectively suggested that under the present experimental conditions, 220 MHz pulsed modulated RF exposure could impair sperm quality in rats, and the disruption of the secreting function of LCs and increased apoptosis of testis cells induced by the RF field might be accounted for by this damaging effect.
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spelling pubmed-64806342019-04-29 Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats Guo, Ling Lin, Jia-Jin Xue, Yi-Zhe An, Guang-Zhou Zhang, Jun-Ping Zhang, Ke-Ying He, Wei Wang, Huan Li, Wei Ding, Gui-Rong Int J Environ Res Public Health Article Under some occupational conditions, workers are inevitably exposed to high-intensity radiofrequency (RF) fields. In this study, we investigated the effects of one-month exposure to a 220 MHz pulsed modulated RF field at the power density of 50 W/m(2) on the sperm quality in male adult rats. The sperm quality was evaluated by measuring the number, abnormality and survival rate of sperm cells. The morphology of testis was examined by hematoxylin–eosin (HE) staining. The levels of secreting factors by Sertoli cells (SCs) and Leydig cells (LCs) were determined by enzyme linked immunosorbent assay (ELISA). The level of cleaved caspase 3 in the testis was detected by immunofluorescence staining. Finally, the expression levels of the apoptosis-related protein (caspase 3, BAX and BCL2) in the testis were assessed by Western blotting. Compared with the sham group, the sperm quality in the RF group decreased significantly. The levels of secreting factors of SCs and the morphology of the testis showed an obvious change after RF exposure. The level of the secreting factor of LCs decreased significantly after RF exposure. The levels of cleaved caspase 3, caspase 3, and the BAX/BCL2 ratio in the testis increased markedly after RF exposure. These data collectively suggested that under the present experimental conditions, 220 MHz pulsed modulated RF exposure could impair sperm quality in rats, and the disruption of the secreting function of LCs and increased apoptosis of testis cells induced by the RF field might be accounted for by this damaging effect. MDPI 2019-04-10 2019-04 /pmc/articles/PMC6480634/ /pubmed/30974849 http://dx.doi.org/10.3390/ijerph16071286 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guo, Ling
Lin, Jia-Jin
Xue, Yi-Zhe
An, Guang-Zhou
Zhang, Jun-Ping
Zhang, Ke-Ying
He, Wei
Wang, Huan
Li, Wei
Ding, Gui-Rong
Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats
title Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats
title_full Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats
title_fullStr Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats
title_full_unstemmed Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats
title_short Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats
title_sort effects of 220 mhz pulsed modulated radiofrequency field on the sperm quality in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480634/
https://www.ncbi.nlm.nih.gov/pubmed/30974849
http://dx.doi.org/10.3390/ijerph16071286
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