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Robust CRISPR/Cas9 Genome Editing of the HUDEP-2 Erythroid Precursor Line Using Plasmids and Single-Stranded Oligonucleotide Donors

The study of cellular processes and gene regulation in terminal erythroid development has been greatly facilitated by the generation of an immortalised erythroid cell line derived from Human Umbilical Derived Erythroid Precursors, termed HUDEP-2 cells. The ability to efficiently genome edit HUDEP-2...

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Detalles Bibliográficos
Autores principales:	Moir-Meyer, Gemma, Cheong, Pak Leng, Olijnik, Aude-Anais, Brown, Jill, Knight, Samantha, King, Andrew, Kurita, Ryo, Nakamura, Yukio, Gibbons, Richard J., Higgs, Douglas R., Buckle, Veronica J., Babbs, Christian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2018
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481050/ https://www.ncbi.nlm.nih.gov/pubmed/31164570 http://dx.doi.org/10.3390/mps1030028

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