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A Software Architecture to Mimic a Ventricular Tachycardia in Intact Murine Hearts by Means of an All-Optical Platform

Optogenetics is an emerging method that uses light to manipulate electrical activity in excitable cells exploiting the interaction between light and light-sensitive depolarizing ion channels, such as channelrhodopsin-2 (ChR2). Initially used in the neuroscience, it has been adopted in cardiac resear...

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Autores principales: Giardini, Francesco, Biasci, Valentina, Scardigli, Marina, Pavone, Francesco S., Bub, Gil, Sacconi, Leonardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481051/
https://www.ncbi.nlm.nih.gov/pubmed/31164591
http://dx.doi.org/10.3390/mps2010007
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author Giardini, Francesco
Biasci, Valentina
Scardigli, Marina
Pavone, Francesco S.
Bub, Gil
Sacconi, Leonardo
author_facet Giardini, Francesco
Biasci, Valentina
Scardigli, Marina
Pavone, Francesco S.
Bub, Gil
Sacconi, Leonardo
author_sort Giardini, Francesco
collection PubMed
description Optogenetics is an emerging method that uses light to manipulate electrical activity in excitable cells exploiting the interaction between light and light-sensitive depolarizing ion channels, such as channelrhodopsin-2 (ChR2). Initially used in the neuroscience, it has been adopted in cardiac research where the expression of ChR2 in cardiac preparations allows optical pacing, resynchronization and defibrillation. Recently, optogenetics has been leveraged to manipulate cardiac electrical activity in the intact heart in real-time. This new approach was applied to simulate a re-entrant circuit across the ventricle. In this technical note, we describe the development and the implementation of a new software package for real-time optogenetic intervention. The package consists of a single LabVIEW program that simultaneously captures images at very high frame rates and delivers precisely timed optogenetic stimuli based on the content of the images. The software implementation guarantees closed-loop optical manipulation at high temporal resolution by processing the raw data in workstation memory. We demonstrate that this strategy allows the simulation of a ventricular tachycardia with high stability and with a negligible loss of data with a temporal resolution of up to 1 ms.
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spelling pubmed-64810512019-05-31 A Software Architecture to Mimic a Ventricular Tachycardia in Intact Murine Hearts by Means of an All-Optical Platform Giardini, Francesco Biasci, Valentina Scardigli, Marina Pavone, Francesco S. Bub, Gil Sacconi, Leonardo Methods Protoc Technical Note Optogenetics is an emerging method that uses light to manipulate electrical activity in excitable cells exploiting the interaction between light and light-sensitive depolarizing ion channels, such as channelrhodopsin-2 (ChR2). Initially used in the neuroscience, it has been adopted in cardiac research where the expression of ChR2 in cardiac preparations allows optical pacing, resynchronization and defibrillation. Recently, optogenetics has been leveraged to manipulate cardiac electrical activity in the intact heart in real-time. This new approach was applied to simulate a re-entrant circuit across the ventricle. In this technical note, we describe the development and the implementation of a new software package for real-time optogenetic intervention. The package consists of a single LabVIEW program that simultaneously captures images at very high frame rates and delivers precisely timed optogenetic stimuli based on the content of the images. The software implementation guarantees closed-loop optical manipulation at high temporal resolution by processing the raw data in workstation memory. We demonstrate that this strategy allows the simulation of a ventricular tachycardia with high stability and with a negligible loss of data with a temporal resolution of up to 1 ms. MDPI 2019-01-08 /pmc/articles/PMC6481051/ /pubmed/31164591 http://dx.doi.org/10.3390/mps2010007 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Technical Note
Giardini, Francesco
Biasci, Valentina
Scardigli, Marina
Pavone, Francesco S.
Bub, Gil
Sacconi, Leonardo
A Software Architecture to Mimic a Ventricular Tachycardia in Intact Murine Hearts by Means of an All-Optical Platform
title A Software Architecture to Mimic a Ventricular Tachycardia in Intact Murine Hearts by Means of an All-Optical Platform
title_full A Software Architecture to Mimic a Ventricular Tachycardia in Intact Murine Hearts by Means of an All-Optical Platform
title_fullStr A Software Architecture to Mimic a Ventricular Tachycardia in Intact Murine Hearts by Means of an All-Optical Platform
title_full_unstemmed A Software Architecture to Mimic a Ventricular Tachycardia in Intact Murine Hearts by Means of an All-Optical Platform
title_short A Software Architecture to Mimic a Ventricular Tachycardia in Intact Murine Hearts by Means of an All-Optical Platform
title_sort software architecture to mimic a ventricular tachycardia in intact murine hearts by means of an all-optical platform
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481051/
https://www.ncbi.nlm.nih.gov/pubmed/31164591
http://dx.doi.org/10.3390/mps2010007
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