DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens

Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease. Here, we present a simple DNAzol-LAMP (loop-mediated isothermal amplification) procedure for the detection of Mycobacterium tub...

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Autores principales: Rodríguez-García, Álvaro, Mares, Rosa E., Muñoz, Patricia L. A., Meléndez-López, Samuel G., Licea-Navarro, Alexei F., Ramos, Marco A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481065/
https://www.ncbi.nlm.nih.gov/pubmed/31164577
http://dx.doi.org/10.3390/mps1040037
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author Rodríguez-García, Álvaro
Mares, Rosa E.
Muñoz, Patricia L. A.
Meléndez-López, Samuel G.
Licea-Navarro, Alexei F.
Ramos, Marco A.
author_facet Rodríguez-García, Álvaro
Mares, Rosa E.
Muñoz, Patricia L. A.
Meléndez-López, Samuel G.
Licea-Navarro, Alexei F.
Ramos, Marco A.
author_sort Rodríguez-García, Álvaro
collection PubMed
description Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease. Here, we present a simple DNAzol-LAMP (loop-mediated isothermal amplification) procedure for the detection of Mycobacterium tuberculosis in sputum specimens. Twenty smear-positive sputum samples were analyzed as follows: (i) Genetic material was extracted by a standard DNAzol protocol, and (ii) mycobacterial DNA was detected by a typical TB-specific loop-mediated isothermal amplification method. Results and diagnostic test performance attests to the suitability of the proposed procedure.
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spelling pubmed-64810652019-05-31 DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens Rodríguez-García, Álvaro Mares, Rosa E. Muñoz, Patricia L. A. Meléndez-López, Samuel G. Licea-Navarro, Alexei F. Ramos, Marco A. Methods Protoc Technical Note Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease. Here, we present a simple DNAzol-LAMP (loop-mediated isothermal amplification) procedure for the detection of Mycobacterium tuberculosis in sputum specimens. Twenty smear-positive sputum samples were analyzed as follows: (i) Genetic material was extracted by a standard DNAzol protocol, and (ii) mycobacterial DNA was detected by a typical TB-specific loop-mediated isothermal amplification method. Results and diagnostic test performance attests to the suitability of the proposed procedure. MDPI 2018-10-23 /pmc/articles/PMC6481065/ /pubmed/31164577 http://dx.doi.org/10.3390/mps1040037 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Technical Note
Rodríguez-García, Álvaro
Mares, Rosa E.
Muñoz, Patricia L. A.
Meléndez-López, Samuel G.
Licea-Navarro, Alexei F.
Ramos, Marco A.
DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens
title DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens
title_full DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens
title_fullStr DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens
title_full_unstemmed DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens
title_short DNA Extraction with DNAzol and LAMP, Performed in a Heating Block as a Simple Procedure for Detection of Mycobacterium tuberculosis in Sputum Specimens
title_sort dna extraction with dnazol and lamp, performed in a heating block as a simple procedure for detection of mycobacterium tuberculosis in sputum specimens
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481065/
https://www.ncbi.nlm.nih.gov/pubmed/31164577
http://dx.doi.org/10.3390/mps1040037
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