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Two-Step Concentration of Complex Water Samples for the Detection of Viruses
The accurate detection and quantification of pathogenic viruses in water is essential to understand and reduce the risk of human infection. This paper describes a two-step method suitable for concentrating viruses in water and wastewater samples. The method involves a tangential flow ultrafiltration...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481077/ https://www.ncbi.nlm.nih.gov/pubmed/31164575 http://dx.doi.org/10.3390/mps1030035 |
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author | Farkas, Kata McDonald, James E. Malham, Shelagh K. Jones, Davey L. |
author_facet | Farkas, Kata McDonald, James E. Malham, Shelagh K. Jones, Davey L. |
author_sort | Farkas, Kata |
collection | PubMed |
description | The accurate detection and quantification of pathogenic viruses in water is essential to understand and reduce the risk of human infection. This paper describes a two-step method suitable for concentrating viruses in water and wastewater samples. The method involves a tangential flow ultrafiltration step that reduces the sample volume of 1–10 L to approximately 50 mL, followed by secondary precipitation using polyethylene glycol 6000, which reduces the volume to 1–4 mL. For method validation, water samples were spiked with different concentrations of enteric viruses, and viral recovery in the concentrates exceeded 10% in all experiments. The method is suitable for water samples with high and low salinity and turbidity, allowing an accurate comparison of viral titers in a diverse range of water types. Furthermore, the method has the potential to concentrate other pathogens, e.g., bacteria or protozoa. Hence, the use of this method can improve the holistic assessment of risks associated with wastewater-contaminated environments. |
format | Online Article Text |
id | pubmed-6481077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64810772019-05-31 Two-Step Concentration of Complex Water Samples for the Detection of Viruses Farkas, Kata McDonald, James E. Malham, Shelagh K. Jones, Davey L. Methods Protoc Protocol The accurate detection and quantification of pathogenic viruses in water is essential to understand and reduce the risk of human infection. This paper describes a two-step method suitable for concentrating viruses in water and wastewater samples. The method involves a tangential flow ultrafiltration step that reduces the sample volume of 1–10 L to approximately 50 mL, followed by secondary precipitation using polyethylene glycol 6000, which reduces the volume to 1–4 mL. For method validation, water samples were spiked with different concentrations of enteric viruses, and viral recovery in the concentrates exceeded 10% in all experiments. The method is suitable for water samples with high and low salinity and turbidity, allowing an accurate comparison of viral titers in a diverse range of water types. Furthermore, the method has the potential to concentrate other pathogens, e.g., bacteria or protozoa. Hence, the use of this method can improve the holistic assessment of risks associated with wastewater-contaminated environments. MDPI 2018-09-10 /pmc/articles/PMC6481077/ /pubmed/31164575 http://dx.doi.org/10.3390/mps1030035 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Farkas, Kata McDonald, James E. Malham, Shelagh K. Jones, Davey L. Two-Step Concentration of Complex Water Samples for the Detection of Viruses |
title | Two-Step Concentration of Complex Water Samples for the Detection of Viruses |
title_full | Two-Step Concentration of Complex Water Samples for the Detection of Viruses |
title_fullStr | Two-Step Concentration of Complex Water Samples for the Detection of Viruses |
title_full_unstemmed | Two-Step Concentration of Complex Water Samples for the Detection of Viruses |
title_short | Two-Step Concentration of Complex Water Samples for the Detection of Viruses |
title_sort | two-step concentration of complex water samples for the detection of viruses |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481077/ https://www.ncbi.nlm.nih.gov/pubmed/31164575 http://dx.doi.org/10.3390/mps1030035 |
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