Cargando…

Two-Step Concentration of Complex Water Samples for the Detection of Viruses

The accurate detection and quantification of pathogenic viruses in water is essential to understand and reduce the risk of human infection. This paper describes a two-step method suitable for concentrating viruses in water and wastewater samples. The method involves a tangential flow ultrafiltration...

Descripción completa

Detalles Bibliográficos
Autores principales: Farkas, Kata, McDonald, James E., Malham, Shelagh K., Jones, Davey L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481077/
https://www.ncbi.nlm.nih.gov/pubmed/31164575
http://dx.doi.org/10.3390/mps1030035
_version_ 1783413711498641408
author Farkas, Kata
McDonald, James E.
Malham, Shelagh K.
Jones, Davey L.
author_facet Farkas, Kata
McDonald, James E.
Malham, Shelagh K.
Jones, Davey L.
author_sort Farkas, Kata
collection PubMed
description The accurate detection and quantification of pathogenic viruses in water is essential to understand and reduce the risk of human infection. This paper describes a two-step method suitable for concentrating viruses in water and wastewater samples. The method involves a tangential flow ultrafiltration step that reduces the sample volume of 1–10 L to approximately 50 mL, followed by secondary precipitation using polyethylene glycol 6000, which reduces the volume to 1–4 mL. For method validation, water samples were spiked with different concentrations of enteric viruses, and viral recovery in the concentrates exceeded 10% in all experiments. The method is suitable for water samples with high and low salinity and turbidity, allowing an accurate comparison of viral titers in a diverse range of water types. Furthermore, the method has the potential to concentrate other pathogens, e.g., bacteria or protozoa. Hence, the use of this method can improve the holistic assessment of risks associated with wastewater-contaminated environments.
format Online
Article
Text
id pubmed-6481077
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64810772019-05-31 Two-Step Concentration of Complex Water Samples for the Detection of Viruses Farkas, Kata McDonald, James E. Malham, Shelagh K. Jones, Davey L. Methods Protoc Protocol The accurate detection and quantification of pathogenic viruses in water is essential to understand and reduce the risk of human infection. This paper describes a two-step method suitable for concentrating viruses in water and wastewater samples. The method involves a tangential flow ultrafiltration step that reduces the sample volume of 1–10 L to approximately 50 mL, followed by secondary precipitation using polyethylene glycol 6000, which reduces the volume to 1–4 mL. For method validation, water samples were spiked with different concentrations of enteric viruses, and viral recovery in the concentrates exceeded 10% in all experiments. The method is suitable for water samples with high and low salinity and turbidity, allowing an accurate comparison of viral titers in a diverse range of water types. Furthermore, the method has the potential to concentrate other pathogens, e.g., bacteria or protozoa. Hence, the use of this method can improve the holistic assessment of risks associated with wastewater-contaminated environments. MDPI 2018-09-10 /pmc/articles/PMC6481077/ /pubmed/31164575 http://dx.doi.org/10.3390/mps1030035 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Farkas, Kata
McDonald, James E.
Malham, Shelagh K.
Jones, Davey L.
Two-Step Concentration of Complex Water Samples for the Detection of Viruses
title Two-Step Concentration of Complex Water Samples for the Detection of Viruses
title_full Two-Step Concentration of Complex Water Samples for the Detection of Viruses
title_fullStr Two-Step Concentration of Complex Water Samples for the Detection of Viruses
title_full_unstemmed Two-Step Concentration of Complex Water Samples for the Detection of Viruses
title_short Two-Step Concentration of Complex Water Samples for the Detection of Viruses
title_sort two-step concentration of complex water samples for the detection of viruses
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481077/
https://www.ncbi.nlm.nih.gov/pubmed/31164575
http://dx.doi.org/10.3390/mps1030035
work_keys_str_mv AT farkaskata twostepconcentrationofcomplexwatersamplesforthedetectionofviruses
AT mcdonaldjamese twostepconcentrationofcomplexwatersamplesforthedetectionofviruses
AT malhamshelaghk twostepconcentrationofcomplexwatersamplesforthedetectionofviruses
AT jonesdaveyl twostepconcentrationofcomplexwatersamplesforthedetectionofviruses