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A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria
This report provides a broadly applicable and cost-effective method for the purification of mycosporine-like amino acids (MAAs) from cyanobacteria. As MAAs are known to have multiple bioactivities for health and beauty, a universal isolation method of MAAs from biomass is attractive. In particular,...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481085/ https://www.ncbi.nlm.nih.gov/pubmed/31164584 http://dx.doi.org/10.3390/mps1040046 |
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author | Ngoennet, Siripat Nishikawa, Yasuhiro Hibino, Takashi Waditee-Sirisattha, Rungaroon Kageyama, Hakuto |
author_facet | Ngoennet, Siripat Nishikawa, Yasuhiro Hibino, Takashi Waditee-Sirisattha, Rungaroon Kageyama, Hakuto |
author_sort | Ngoennet, Siripat |
collection | PubMed |
description | This report provides a broadly applicable and cost-effective method for the purification of mycosporine-like amino acids (MAAs) from cyanobacteria. As MAAs are known to have multiple bioactivities for health and beauty, a universal isolation method of MAAs from biomass is attractive. In particular, the biomass of photosynthetic microorganisms such as cyanobacteria is of interest as a natural source of useful compound production, because of their photoautotrophic property. The method presented here is applicable for the isolation of mycosporine-2-glycine (M2G), which is a rare MAA produced in a halotolerant cyanobacterium. This method also allowed for the isolation of two of the most common MAAs, shinorine (SHI) and porphyra-334 (P334). A three-step separation process using low pressure liquid chromatography yielded purified MAAs, which were characterized by nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC/MS) analyses. The purified MAAs exhibited free radical scavenging activity in the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. The experimental parameters obtained in this report may allow for a scale-up of the MAA purification process for future industrial applications. |
format | Online Article Text |
id | pubmed-6481085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64810852019-05-31 A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria Ngoennet, Siripat Nishikawa, Yasuhiro Hibino, Takashi Waditee-Sirisattha, Rungaroon Kageyama, Hakuto Methods Protoc Protocol This report provides a broadly applicable and cost-effective method for the purification of mycosporine-like amino acids (MAAs) from cyanobacteria. As MAAs are known to have multiple bioactivities for health and beauty, a universal isolation method of MAAs from biomass is attractive. In particular, the biomass of photosynthetic microorganisms such as cyanobacteria is of interest as a natural source of useful compound production, because of their photoautotrophic property. The method presented here is applicable for the isolation of mycosporine-2-glycine (M2G), which is a rare MAA produced in a halotolerant cyanobacterium. This method also allowed for the isolation of two of the most common MAAs, shinorine (SHI) and porphyra-334 (P334). A three-step separation process using low pressure liquid chromatography yielded purified MAAs, which were characterized by nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC/MS) analyses. The purified MAAs exhibited free radical scavenging activity in the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. The experimental parameters obtained in this report may allow for a scale-up of the MAA purification process for future industrial applications. MDPI 2018-12-03 /pmc/articles/PMC6481085/ /pubmed/31164584 http://dx.doi.org/10.3390/mps1040046 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Ngoennet, Siripat Nishikawa, Yasuhiro Hibino, Takashi Waditee-Sirisattha, Rungaroon Kageyama, Hakuto A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria |
title | A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria |
title_full | A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria |
title_fullStr | A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria |
title_full_unstemmed | A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria |
title_short | A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria |
title_sort | method for the isolation and characterization of mycosporine-like amino acids from cyanobacteria |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481085/ https://www.ncbi.nlm.nih.gov/pubmed/31164584 http://dx.doi.org/10.3390/mps1040046 |
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