Cargando…

A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria

This report provides a broadly applicable and cost-effective method for the purification of mycosporine-like amino acids (MAAs) from cyanobacteria. As MAAs are known to have multiple bioactivities for health and beauty, a universal isolation method of MAAs from biomass is attractive. In particular,...

Descripción completa

Detalles Bibliográficos
Autores principales: Ngoennet, Siripat, Nishikawa, Yasuhiro, Hibino, Takashi, Waditee-Sirisattha, Rungaroon, Kageyama, Hakuto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481085/
https://www.ncbi.nlm.nih.gov/pubmed/31164584
http://dx.doi.org/10.3390/mps1040046
_version_ 1783413713131274240
author Ngoennet, Siripat
Nishikawa, Yasuhiro
Hibino, Takashi
Waditee-Sirisattha, Rungaroon
Kageyama, Hakuto
author_facet Ngoennet, Siripat
Nishikawa, Yasuhiro
Hibino, Takashi
Waditee-Sirisattha, Rungaroon
Kageyama, Hakuto
author_sort Ngoennet, Siripat
collection PubMed
description This report provides a broadly applicable and cost-effective method for the purification of mycosporine-like amino acids (MAAs) from cyanobacteria. As MAAs are known to have multiple bioactivities for health and beauty, a universal isolation method of MAAs from biomass is attractive. In particular, the biomass of photosynthetic microorganisms such as cyanobacteria is of interest as a natural source of useful compound production, because of their photoautotrophic property. The method presented here is applicable for the isolation of mycosporine-2-glycine (M2G), which is a rare MAA produced in a halotolerant cyanobacterium. This method also allowed for the isolation of two of the most common MAAs, shinorine (SHI) and porphyra-334 (P334). A three-step separation process using low pressure liquid chromatography yielded purified MAAs, which were characterized by nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC/MS) analyses. The purified MAAs exhibited free radical scavenging activity in the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. The experimental parameters obtained in this report may allow for a scale-up of the MAA purification process for future industrial applications.
format Online
Article
Text
id pubmed-6481085
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-64810852019-05-31 A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria Ngoennet, Siripat Nishikawa, Yasuhiro Hibino, Takashi Waditee-Sirisattha, Rungaroon Kageyama, Hakuto Methods Protoc Protocol This report provides a broadly applicable and cost-effective method for the purification of mycosporine-like amino acids (MAAs) from cyanobacteria. As MAAs are known to have multiple bioactivities for health and beauty, a universal isolation method of MAAs from biomass is attractive. In particular, the biomass of photosynthetic microorganisms such as cyanobacteria is of interest as a natural source of useful compound production, because of their photoautotrophic property. The method presented here is applicable for the isolation of mycosporine-2-glycine (M2G), which is a rare MAA produced in a halotolerant cyanobacterium. This method also allowed for the isolation of two of the most common MAAs, shinorine (SHI) and porphyra-334 (P334). A three-step separation process using low pressure liquid chromatography yielded purified MAAs, which were characterized by nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC/MS) analyses. The purified MAAs exhibited free radical scavenging activity in the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. The experimental parameters obtained in this report may allow for a scale-up of the MAA purification process for future industrial applications. MDPI 2018-12-03 /pmc/articles/PMC6481085/ /pubmed/31164584 http://dx.doi.org/10.3390/mps1040046 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Ngoennet, Siripat
Nishikawa, Yasuhiro
Hibino, Takashi
Waditee-Sirisattha, Rungaroon
Kageyama, Hakuto
A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria
title A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria
title_full A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria
title_fullStr A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria
title_full_unstemmed A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria
title_short A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria
title_sort method for the isolation and characterization of mycosporine-like amino acids from cyanobacteria
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481085/
https://www.ncbi.nlm.nih.gov/pubmed/31164584
http://dx.doi.org/10.3390/mps1040046
work_keys_str_mv AT ngoennetsiripat amethodfortheisolationandcharacterizationofmycosporinelikeaminoacidsfromcyanobacteria
AT nishikawayasuhiro amethodfortheisolationandcharacterizationofmycosporinelikeaminoacidsfromcyanobacteria
AT hibinotakashi amethodfortheisolationandcharacterizationofmycosporinelikeaminoacidsfromcyanobacteria
AT waditeesirisattharungaroon amethodfortheisolationandcharacterizationofmycosporinelikeaminoacidsfromcyanobacteria
AT kageyamahakuto amethodfortheisolationandcharacterizationofmycosporinelikeaminoacidsfromcyanobacteria
AT ngoennetsiripat methodfortheisolationandcharacterizationofmycosporinelikeaminoacidsfromcyanobacteria
AT nishikawayasuhiro methodfortheisolationandcharacterizationofmycosporinelikeaminoacidsfromcyanobacteria
AT hibinotakashi methodfortheisolationandcharacterizationofmycosporinelikeaminoacidsfromcyanobacteria
AT waditeesirisattharungaroon methodfortheisolationandcharacterizationofmycosporinelikeaminoacidsfromcyanobacteria
AT kageyamahakuto methodfortheisolationandcharacterizationofmycosporinelikeaminoacidsfromcyanobacteria