Improved Detection and Fragmentation of Disulphide-Linked Peptides

Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ioni...

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Detalles Bibliográficos
Autores principales: Maes, Evelyne, Clerens, Stefan, Dyer, Jolon M., Deb-Choudhury, Santanu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481087/
https://www.ncbi.nlm.nih.gov/pubmed/31164574
http://dx.doi.org/10.3390/mps1030033
Descripción
Sumario:Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required.

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