Improved Detection and Fragmentation of Disulphide-Linked Peptides

Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ioni...

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Autores principales: Maes, Evelyne, Clerens, Stefan, Dyer, Jolon M., Deb-Choudhury, Santanu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481087/
https://www.ncbi.nlm.nih.gov/pubmed/31164574
http://dx.doi.org/10.3390/mps1030033
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author Maes, Evelyne
Clerens, Stefan
Dyer, Jolon M.
Deb-Choudhury, Santanu
author_facet Maes, Evelyne
Clerens, Stefan
Dyer, Jolon M.
Deb-Choudhury, Santanu
author_sort Maes, Evelyne
collection PubMed
description Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required.
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spelling pubmed-64810872019-05-31 Improved Detection and Fragmentation of Disulphide-Linked Peptides Maes, Evelyne Clerens, Stefan Dyer, Jolon M. Deb-Choudhury, Santanu Methods Protoc Technical Note Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required. MDPI 2018-09-03 /pmc/articles/PMC6481087/ /pubmed/31164574 http://dx.doi.org/10.3390/mps1030033 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Technical Note
Maes, Evelyne
Clerens, Stefan
Dyer, Jolon M.
Deb-Choudhury, Santanu
Improved Detection and Fragmentation of Disulphide-Linked Peptides
title Improved Detection and Fragmentation of Disulphide-Linked Peptides
title_full Improved Detection and Fragmentation of Disulphide-Linked Peptides
title_fullStr Improved Detection and Fragmentation of Disulphide-Linked Peptides
title_full_unstemmed Improved Detection and Fragmentation of Disulphide-Linked Peptides
title_short Improved Detection and Fragmentation of Disulphide-Linked Peptides
title_sort improved detection and fragmentation of disulphide-linked peptides
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481087/
https://www.ncbi.nlm.nih.gov/pubmed/31164574
http://dx.doi.org/10.3390/mps1030033
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