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A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis
Atherosclerosis, a chronic inflammatory disorder characterized by endothelial dysfunction and blood vessel narrowing, is the leading cause of cardiovascular diseases including heart attack and stroke. Herein, we present a novel tunable microfluidic atherosclerosis model to study vascular inflammatio...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481702/ https://www.ncbi.nlm.nih.gov/pubmed/31069288 http://dx.doi.org/10.1063/1.4993762 |
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author | Venugopal Menon, Nishanth Tay, Hui Min Pang, Kuin Tian Dalan, Rinkoo Wong, Siew Cheng Wang, Xiaomeng Li, King Ho Holden Hou, Han Wei |
author_facet | Venugopal Menon, Nishanth Tay, Hui Min Pang, Kuin Tian Dalan, Rinkoo Wong, Siew Cheng Wang, Xiaomeng Li, King Ho Holden Hou, Han Wei |
author_sort | Venugopal Menon, Nishanth |
collection | PubMed |
description | Atherosclerosis, a chronic inflammatory disorder characterized by endothelial dysfunction and blood vessel narrowing, is the leading cause of cardiovascular diseases including heart attack and stroke. Herein, we present a novel tunable microfluidic atherosclerosis model to study vascular inflammation and leukocyte-endothelial interactions in 3D vessel stenosis. Flow and shear stress profiles were characterized in pneumatic-controlled stenosis conditions (0%, 50% and 80% constriction) using fluid simulation and experimental beads perfusion. Due to non-uniform fluid flow at the 3D stenosis, distinct monocyte (THP-1) adhesion patterns on inflamed [tumor necrosis factor-α (TNF-α) treated] endothelium were observed, and there was a differential endothelial expression of intercellular adhesion molecule-1 (ICAM-1) at the constriction region. Whole blood perfusion studies also showed increased leukocyte interactions (cell rolling and adherence) at the stenosis of healthy and inflamed endothelium, clearly highlighting the importance of vascular inflammation, flow disturbance, and vessel geometry in recapitulating atherogenic microenvironment. To demonstrate inflammatory risk assessment using leukocytes as functional biomarkers, we perfused whole blood samples into the developed microdevices (80% constriction) and observed significant dose-dependent effects of leukocyte adhesion in healthy and inflamed (TNF-α treated) blood samples. Taken together, the 3D stenosis chip facilitates quantitative study of hemodynamics and leukocyte-endothelial interactions, and can be further developed into a point-of-care blood profiling device for atherosclerosis and other vascular diseases. |
format | Online Article Text |
id | pubmed-6481702 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-64817022019-05-08 A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis Venugopal Menon, Nishanth Tay, Hui Min Pang, Kuin Tian Dalan, Rinkoo Wong, Siew Cheng Wang, Xiaomeng Li, King Ho Holden Hou, Han Wei APL Bioeng Articles Atherosclerosis, a chronic inflammatory disorder characterized by endothelial dysfunction and blood vessel narrowing, is the leading cause of cardiovascular diseases including heart attack and stroke. Herein, we present a novel tunable microfluidic atherosclerosis model to study vascular inflammation and leukocyte-endothelial interactions in 3D vessel stenosis. Flow and shear stress profiles were characterized in pneumatic-controlled stenosis conditions (0%, 50% and 80% constriction) using fluid simulation and experimental beads perfusion. Due to non-uniform fluid flow at the 3D stenosis, distinct monocyte (THP-1) adhesion patterns on inflamed [tumor necrosis factor-α (TNF-α) treated] endothelium were observed, and there was a differential endothelial expression of intercellular adhesion molecule-1 (ICAM-1) at the constriction region. Whole blood perfusion studies also showed increased leukocyte interactions (cell rolling and adherence) at the stenosis of healthy and inflamed endothelium, clearly highlighting the importance of vascular inflammation, flow disturbance, and vessel geometry in recapitulating atherogenic microenvironment. To demonstrate inflammatory risk assessment using leukocytes as functional biomarkers, we perfused whole blood samples into the developed microdevices (80% constriction) and observed significant dose-dependent effects of leukocyte adhesion in healthy and inflamed (TNF-α treated) blood samples. Taken together, the 3D stenosis chip facilitates quantitative study of hemodynamics and leukocyte-endothelial interactions, and can be further developed into a point-of-care blood profiling device for atherosclerosis and other vascular diseases. AIP Publishing LLC 2018-01-02 /pmc/articles/PMC6481702/ /pubmed/31069288 http://dx.doi.org/10.1063/1.4993762 Text en © 2018 Author(s). 2473-2877/2018/2(1)/016103/12 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Articles Venugopal Menon, Nishanth Tay, Hui Min Pang, Kuin Tian Dalan, Rinkoo Wong, Siew Cheng Wang, Xiaomeng Li, King Ho Holden Hou, Han Wei A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis |
title | A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis |
title_full | A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis |
title_fullStr | A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis |
title_full_unstemmed | A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis |
title_short | A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis |
title_sort | tunable microfluidic 3d stenosis model to study leukocyte-endothelial interactions in atherosclerosis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481702/ https://www.ncbi.nlm.nih.gov/pubmed/31069288 http://dx.doi.org/10.1063/1.4993762 |
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