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A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis

Atherosclerosis, a chronic inflammatory disorder characterized by endothelial dysfunction and blood vessel narrowing, is the leading cause of cardiovascular diseases including heart attack and stroke. Herein, we present a novel tunable microfluidic atherosclerosis model to study vascular inflammatio...

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Autores principales: Venugopal Menon, Nishanth, Tay, Hui Min, Pang, Kuin Tian, Dalan, Rinkoo, Wong, Siew Cheng, Wang, Xiaomeng, Li, King Ho Holden, Hou, Han Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481702/
https://www.ncbi.nlm.nih.gov/pubmed/31069288
http://dx.doi.org/10.1063/1.4993762
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author Venugopal Menon, Nishanth
Tay, Hui Min
Pang, Kuin Tian
Dalan, Rinkoo
Wong, Siew Cheng
Wang, Xiaomeng
Li, King Ho Holden
Hou, Han Wei
author_facet Venugopal Menon, Nishanth
Tay, Hui Min
Pang, Kuin Tian
Dalan, Rinkoo
Wong, Siew Cheng
Wang, Xiaomeng
Li, King Ho Holden
Hou, Han Wei
author_sort Venugopal Menon, Nishanth
collection PubMed
description Atherosclerosis, a chronic inflammatory disorder characterized by endothelial dysfunction and blood vessel narrowing, is the leading cause of cardiovascular diseases including heart attack and stroke. Herein, we present a novel tunable microfluidic atherosclerosis model to study vascular inflammation and leukocyte-endothelial interactions in 3D vessel stenosis. Flow and shear stress profiles were characterized in pneumatic-controlled stenosis conditions (0%, 50% and 80% constriction) using fluid simulation and experimental beads perfusion. Due to non-uniform fluid flow at the 3D stenosis, distinct monocyte (THP-1) adhesion patterns on inflamed [tumor necrosis factor-α (TNF-α) treated] endothelium were observed, and there was a differential endothelial expression of intercellular adhesion molecule-1 (ICAM-1) at the constriction region. Whole blood perfusion studies also showed increased leukocyte interactions (cell rolling and adherence) at the stenosis of healthy and inflamed endothelium, clearly highlighting the importance of vascular inflammation, flow disturbance, and vessel geometry in recapitulating atherogenic microenvironment. To demonstrate inflammatory risk assessment using leukocytes as functional biomarkers, we perfused whole blood samples into the developed microdevices (80% constriction) and observed significant dose-dependent effects of leukocyte adhesion in healthy and inflamed (TNF-α treated) blood samples. Taken together, the 3D stenosis chip facilitates quantitative study of hemodynamics and leukocyte-endothelial interactions, and can be further developed into a point-of-care blood profiling device for atherosclerosis and other vascular diseases.
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spelling pubmed-64817022019-05-08 A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis Venugopal Menon, Nishanth Tay, Hui Min Pang, Kuin Tian Dalan, Rinkoo Wong, Siew Cheng Wang, Xiaomeng Li, King Ho Holden Hou, Han Wei APL Bioeng Articles Atherosclerosis, a chronic inflammatory disorder characterized by endothelial dysfunction and blood vessel narrowing, is the leading cause of cardiovascular diseases including heart attack and stroke. Herein, we present a novel tunable microfluidic atherosclerosis model to study vascular inflammation and leukocyte-endothelial interactions in 3D vessel stenosis. Flow and shear stress profiles were characterized in pneumatic-controlled stenosis conditions (0%, 50% and 80% constriction) using fluid simulation and experimental beads perfusion. Due to non-uniform fluid flow at the 3D stenosis, distinct monocyte (THP-1) adhesion patterns on inflamed [tumor necrosis factor-α (TNF-α) treated] endothelium were observed, and there was a differential endothelial expression of intercellular adhesion molecule-1 (ICAM-1) at the constriction region. Whole blood perfusion studies also showed increased leukocyte interactions (cell rolling and adherence) at the stenosis of healthy and inflamed endothelium, clearly highlighting the importance of vascular inflammation, flow disturbance, and vessel geometry in recapitulating atherogenic microenvironment. To demonstrate inflammatory risk assessment using leukocytes as functional biomarkers, we perfused whole blood samples into the developed microdevices (80% constriction) and observed significant dose-dependent effects of leukocyte adhesion in healthy and inflamed (TNF-α treated) blood samples. Taken together, the 3D stenosis chip facilitates quantitative study of hemodynamics and leukocyte-endothelial interactions, and can be further developed into a point-of-care blood profiling device for atherosclerosis and other vascular diseases. AIP Publishing LLC 2018-01-02 /pmc/articles/PMC6481702/ /pubmed/31069288 http://dx.doi.org/10.1063/1.4993762 Text en © 2018 Author(s). 2473-2877/2018/2(1)/016103/12 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Articles
Venugopal Menon, Nishanth
Tay, Hui Min
Pang, Kuin Tian
Dalan, Rinkoo
Wong, Siew Cheng
Wang, Xiaomeng
Li, King Ho Holden
Hou, Han Wei
A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis
title A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis
title_full A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis
title_fullStr A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis
title_full_unstemmed A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis
title_short A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis
title_sort tunable microfluidic 3d stenosis model to study leukocyte-endothelial interactions in atherosclerosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481702/
https://www.ncbi.nlm.nih.gov/pubmed/31069288
http://dx.doi.org/10.1063/1.4993762
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