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Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets

We report an experimental approach to study the mechanosensitivity of cell-cell contact upon mechanical stimulation in suspended cell-doublets. The doublet is placed astride an hourglass aperture, and a hydrodynamic force is selectively exerted on only one of the cells. The geometry of the device co...

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Detalles Bibliográficos
Autores principales: Gao, Xumei, Acharya, Bipul R., Engl, Wilfried Claude Otto, De Mets, Richard, Thiery, Jean Paul, Yap, Alpha S., Viasnoff, Virgile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481720/
https://www.ncbi.nlm.nih.gov/pubmed/31069308
http://dx.doi.org/10.1063/1.5025216
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author Gao, Xumei
Acharya, Bipul R.
Engl, Wilfried Claude Otto
De Mets, Richard
Thiery, Jean Paul
Yap, Alpha S.
Viasnoff, Virgile
author_facet Gao, Xumei
Acharya, Bipul R.
Engl, Wilfried Claude Otto
De Mets, Richard
Thiery, Jean Paul
Yap, Alpha S.
Viasnoff, Virgile
author_sort Gao, Xumei
collection PubMed
description We report an experimental approach to study the mechanosensitivity of cell-cell contact upon mechanical stimulation in suspended cell-doublets. The doublet is placed astride an hourglass aperture, and a hydrodynamic force is selectively exerted on only one of the cells. The geometry of the device concentrates the mechanical shear over the junction area. Together with mechanical shear, the system also allows confocal quantitative live imaging of the recruitment of junction proteins (e.g., E-cadherin, ZO-1, occludin, and actin). We observed the time sequence over which proteins were recruited to the stretched region of the contact. The compressed side of the contact showed no response. We demonstrated how this mechanism polarizes the stress-induced recruitment of junctional components within one single junction. Finally, we demonstrated that stabilizing the actin cortex dynamics abolishes the mechanosensitive response of the junction. Our experimental design provides an original approach to study the role of mechanical force at a cell-cell contact with unprecedented control over stress application and quantitative optical analysis.
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spelling pubmed-64817202019-05-08 Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets Gao, Xumei Acharya, Bipul R. Engl, Wilfried Claude Otto De Mets, Richard Thiery, Jean Paul Yap, Alpha S. Viasnoff, Virgile APL Bioeng Articles We report an experimental approach to study the mechanosensitivity of cell-cell contact upon mechanical stimulation in suspended cell-doublets. The doublet is placed astride an hourglass aperture, and a hydrodynamic force is selectively exerted on only one of the cells. The geometry of the device concentrates the mechanical shear over the junction area. Together with mechanical shear, the system also allows confocal quantitative live imaging of the recruitment of junction proteins (e.g., E-cadherin, ZO-1, occludin, and actin). We observed the time sequence over which proteins were recruited to the stretched region of the contact. The compressed side of the contact showed no response. We demonstrated how this mechanism polarizes the stress-induced recruitment of junctional components within one single junction. Finally, we demonstrated that stabilizing the actin cortex dynamics abolishes the mechanosensitive response of the junction. Our experimental design provides an original approach to study the role of mechanical force at a cell-cell contact with unprecedented control over stress application and quantitative optical analysis. AIP Publishing LLC 2018-06-19 /pmc/articles/PMC6481720/ /pubmed/31069308 http://dx.doi.org/10.1063/1.5025216 Text en © 2018 Author(s). 2473-2877/2018/2(2)/026111/14 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Articles
Gao, Xumei
Acharya, Bipul R.
Engl, Wilfried Claude Otto
De Mets, Richard
Thiery, Jean Paul
Yap, Alpha S.
Viasnoff, Virgile
Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets
title Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets
title_full Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets
title_fullStr Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets
title_full_unstemmed Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets
title_short Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets
title_sort probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481720/
https://www.ncbi.nlm.nih.gov/pubmed/31069308
http://dx.doi.org/10.1063/1.5025216
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