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Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets
We report an experimental approach to study the mechanosensitivity of cell-cell contact upon mechanical stimulation in suspended cell-doublets. The doublet is placed astride an hourglass aperture, and a hydrodynamic force is selectively exerted on only one of the cells. The geometry of the device co...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481720/ https://www.ncbi.nlm.nih.gov/pubmed/31069308 http://dx.doi.org/10.1063/1.5025216 |
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author | Gao, Xumei Acharya, Bipul R. Engl, Wilfried Claude Otto De Mets, Richard Thiery, Jean Paul Yap, Alpha S. Viasnoff, Virgile |
author_facet | Gao, Xumei Acharya, Bipul R. Engl, Wilfried Claude Otto De Mets, Richard Thiery, Jean Paul Yap, Alpha S. Viasnoff, Virgile |
author_sort | Gao, Xumei |
collection | PubMed |
description | We report an experimental approach to study the mechanosensitivity of cell-cell contact upon mechanical stimulation in suspended cell-doublets. The doublet is placed astride an hourglass aperture, and a hydrodynamic force is selectively exerted on only one of the cells. The geometry of the device concentrates the mechanical shear over the junction area. Together with mechanical shear, the system also allows confocal quantitative live imaging of the recruitment of junction proteins (e.g., E-cadherin, ZO-1, occludin, and actin). We observed the time sequence over which proteins were recruited to the stretched region of the contact. The compressed side of the contact showed no response. We demonstrated how this mechanism polarizes the stress-induced recruitment of junctional components within one single junction. Finally, we demonstrated that stabilizing the actin cortex dynamics abolishes the mechanosensitive response of the junction. Our experimental design provides an original approach to study the role of mechanical force at a cell-cell contact with unprecedented control over stress application and quantitative optical analysis. |
format | Online Article Text |
id | pubmed-6481720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-64817202019-05-08 Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets Gao, Xumei Acharya, Bipul R. Engl, Wilfried Claude Otto De Mets, Richard Thiery, Jean Paul Yap, Alpha S. Viasnoff, Virgile APL Bioeng Articles We report an experimental approach to study the mechanosensitivity of cell-cell contact upon mechanical stimulation in suspended cell-doublets. The doublet is placed astride an hourglass aperture, and a hydrodynamic force is selectively exerted on only one of the cells. The geometry of the device concentrates the mechanical shear over the junction area. Together with mechanical shear, the system also allows confocal quantitative live imaging of the recruitment of junction proteins (e.g., E-cadherin, ZO-1, occludin, and actin). We observed the time sequence over which proteins were recruited to the stretched region of the contact. The compressed side of the contact showed no response. We demonstrated how this mechanism polarizes the stress-induced recruitment of junctional components within one single junction. Finally, we demonstrated that stabilizing the actin cortex dynamics abolishes the mechanosensitive response of the junction. Our experimental design provides an original approach to study the role of mechanical force at a cell-cell contact with unprecedented control over stress application and quantitative optical analysis. AIP Publishing LLC 2018-06-19 /pmc/articles/PMC6481720/ /pubmed/31069308 http://dx.doi.org/10.1063/1.5025216 Text en © 2018 Author(s). 2473-2877/2018/2(2)/026111/14 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Articles Gao, Xumei Acharya, Bipul R. Engl, Wilfried Claude Otto De Mets, Richard Thiery, Jean Paul Yap, Alpha S. Viasnoff, Virgile Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets |
title | Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets |
title_full | Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets |
title_fullStr | Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets |
title_full_unstemmed | Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets |
title_short | Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets |
title_sort | probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481720/ https://www.ncbi.nlm.nih.gov/pubmed/31069308 http://dx.doi.org/10.1063/1.5025216 |
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