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Multi-sample deformability cytometry of cancer cells

There is growing recognition that cell deformability can play an important role in cancer metastasis and diagnostics. Advancement of methods to characterize cell deformability in a high throughput manner and the capacity to process numerous samples can impact cancer-related applications ranging from...

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Autores principales: Ahmmed, Shamim M., Bithi, Swastika S., Pore, Adity A., Mubtasim, Noshin, Schuster, Caroline, Gollahon, Lauren S., Vanapalli, Siva A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481721/
https://www.ncbi.nlm.nih.gov/pubmed/31069319
http://dx.doi.org/10.1063/1.5020992
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author Ahmmed, Shamim M.
Bithi, Swastika S.
Pore, Adity A.
Mubtasim, Noshin
Schuster, Caroline
Gollahon, Lauren S.
Vanapalli, Siva A.
author_facet Ahmmed, Shamim M.
Bithi, Swastika S.
Pore, Adity A.
Mubtasim, Noshin
Schuster, Caroline
Gollahon, Lauren S.
Vanapalli, Siva A.
author_sort Ahmmed, Shamim M.
collection PubMed
description There is growing recognition that cell deformability can play an important role in cancer metastasis and diagnostics. Advancement of methods to characterize cell deformability in a high throughput manner and the capacity to process numerous samples can impact cancer-related applications ranging from analysis of patient samples to discovery of anti-cancer compounds to screening of oncogenes. In this study, we report a microfluidic technique called multi-sample deformability cytometry (MS-DC) that allows simultaneous measurement of flow-induced deformation of cells in multiple samples at single-cell resolution using a combination of on-chip reservoirs, distributed pressure control, and data analysis system. Cells are introduced at rates of O(100) cells per second with a data processing speed of 10 min per sample. To validate MS-DC, we tested more than 50 cell-samples that include cancer cell lines with different metastatic potential and cells treated with several cytoskeletal-intervention drugs. Results from MS-DC show that (i) the cell deformability correlates with metastatic potential for both breast and prostate cancer cells but not with their molecular histotype, (ii) the strongly metastatic breast cancer cells have higher deformability than the weakly metastatic ones; however, the strongly metastatic prostate cancer cells have lower deformability than the weakly metastatic counterparts, and (iii) drug-induced disruption of the actin network, microtubule network, and actomyosin contractility increased cancer cell deformability, but stabilization of the cytoskeletal proteins does not alter deformability significantly. Our study demonstrates the capacity of MS-DC to mechanically phenotype tumor cells simultaneously in many samples for cancer research.
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spelling pubmed-64817212019-05-08 Multi-sample deformability cytometry of cancer cells Ahmmed, Shamim M. Bithi, Swastika S. Pore, Adity A. Mubtasim, Noshin Schuster, Caroline Gollahon, Lauren S. Vanapalli, Siva A. APL Bioeng Special Topic: Bioengineering of Cancer There is growing recognition that cell deformability can play an important role in cancer metastasis and diagnostics. Advancement of methods to characterize cell deformability in a high throughput manner and the capacity to process numerous samples can impact cancer-related applications ranging from analysis of patient samples to discovery of anti-cancer compounds to screening of oncogenes. In this study, we report a microfluidic technique called multi-sample deformability cytometry (MS-DC) that allows simultaneous measurement of flow-induced deformation of cells in multiple samples at single-cell resolution using a combination of on-chip reservoirs, distributed pressure control, and data analysis system. Cells are introduced at rates of O(100) cells per second with a data processing speed of 10 min per sample. To validate MS-DC, we tested more than 50 cell-samples that include cancer cell lines with different metastatic potential and cells treated with several cytoskeletal-intervention drugs. Results from MS-DC show that (i) the cell deformability correlates with metastatic potential for both breast and prostate cancer cells but not with their molecular histotype, (ii) the strongly metastatic breast cancer cells have higher deformability than the weakly metastatic ones; however, the strongly metastatic prostate cancer cells have lower deformability than the weakly metastatic counterparts, and (iii) drug-induced disruption of the actin network, microtubule network, and actomyosin contractility increased cancer cell deformability, but stabilization of the cytoskeletal proteins does not alter deformability significantly. Our study demonstrates the capacity of MS-DC to mechanically phenotype tumor cells simultaneously in many samples for cancer research. AIP Publishing LLC 2018-06-21 /pmc/articles/PMC6481721/ /pubmed/31069319 http://dx.doi.org/10.1063/1.5020992 Text en © 2018 Author(s). 2473-2877/2018/2(3)/032002/14 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Special Topic: Bioengineering of Cancer
Ahmmed, Shamim M.
Bithi, Swastika S.
Pore, Adity A.
Mubtasim, Noshin
Schuster, Caroline
Gollahon, Lauren S.
Vanapalli, Siva A.
Multi-sample deformability cytometry of cancer cells
title Multi-sample deformability cytometry of cancer cells
title_full Multi-sample deformability cytometry of cancer cells
title_fullStr Multi-sample deformability cytometry of cancer cells
title_full_unstemmed Multi-sample deformability cytometry of cancer cells
title_short Multi-sample deformability cytometry of cancer cells
title_sort multi-sample deformability cytometry of cancer cells
topic Special Topic: Bioengineering of Cancer
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481721/
https://www.ncbi.nlm.nih.gov/pubmed/31069319
http://dx.doi.org/10.1063/1.5020992
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