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Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction

The aim of this study was to evaluate the effects of exercise training (ET) on the aortic vascular reactivity of ovariectomized and infarcted rats. The animals were divided into 5 groups: Control, Ovariectomized + SHAM sedentary (OVX+SHAM(SED)), OVX+SHAM and ET (OVX+SHAM(ET)), OVX + Myocardial Infar...

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Autores principales: de Oliveira, Suelen Guedes, Claudio, Erick Roberto Gonçalves, de Almeida, Simone Alves, Mengal, Vinicius, da Silva, Fabricio Bragança, Silva, Nyam Florêncio, Mauad, Helder, de Abreu, Glaucia Rodrigues
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481839/
https://www.ncbi.nlm.nih.gov/pubmed/31017961
http://dx.doi.org/10.1371/journal.pone.0215568
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author de Oliveira, Suelen Guedes
Claudio, Erick Roberto Gonçalves
de Almeida, Simone Alves
Mengal, Vinicius
da Silva, Fabricio Bragança
Silva, Nyam Florêncio
Mauad, Helder
de Abreu, Glaucia Rodrigues
author_facet de Oliveira, Suelen Guedes
Claudio, Erick Roberto Gonçalves
de Almeida, Simone Alves
Mengal, Vinicius
da Silva, Fabricio Bragança
Silva, Nyam Florêncio
Mauad, Helder
de Abreu, Glaucia Rodrigues
author_sort de Oliveira, Suelen Guedes
collection PubMed
description The aim of this study was to evaluate the effects of exercise training (ET) on the aortic vascular reactivity of ovariectomized and infarcted rats. The animals were divided into 5 groups: Control, Ovariectomized + SHAM sedentary (OVX+SHAM(SED)), OVX+SHAM and ET (OVX+SHAM(ET)), OVX + Myocardial Infarction sedentary (OVX+MI(SED)), and OVX + MI and ET (OVX+MI(ET)). ET protocol (60 minutes/day, 5x/week) in a motorized treadmill began 15 days after MI and lasted 8 weeks. The endothelium-dependent and endothelium-independent vascular reactivity were evaluated as well as the role of the reactive oxygen species (ROS). Superoxide and nitric oxide (NO) production were analyzed in situ using DHE and DAF-2 fluorescence, respectively. The expression of gp91phox and of the antioxidant enzymes were evaluated by western blotting in the thoracic aorta samples. MI promoted a significant increase in the contractile response and impaired endothelium-mediated relaxation. However, ET prevented the impairment in the vascular reactivity in MI animals. In addition, the protein expression of gp91phox and superoxide production increased and the NO production decreased in the OVX+MI(SED) group but not in the OVX+MI(ET) group. Therefore, ET improves vascular reactivity in MI ovariectomized rats by preventing the increase in the expression of gp91phox and the decrease in the antioxidant enzymes, resulting in a normal ROS and NO production. Thus, ET can be an effective therapeutic strategy for improving the MI-induced vascular alterations in estrogen deficiency condition.
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spelling pubmed-64818392019-05-07 Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction de Oliveira, Suelen Guedes Claudio, Erick Roberto Gonçalves de Almeida, Simone Alves Mengal, Vinicius da Silva, Fabricio Bragança Silva, Nyam Florêncio Mauad, Helder de Abreu, Glaucia Rodrigues PLoS One Research Article The aim of this study was to evaluate the effects of exercise training (ET) on the aortic vascular reactivity of ovariectomized and infarcted rats. The animals were divided into 5 groups: Control, Ovariectomized + SHAM sedentary (OVX+SHAM(SED)), OVX+SHAM and ET (OVX+SHAM(ET)), OVX + Myocardial Infarction sedentary (OVX+MI(SED)), and OVX + MI and ET (OVX+MI(ET)). ET protocol (60 minutes/day, 5x/week) in a motorized treadmill began 15 days after MI and lasted 8 weeks. The endothelium-dependent and endothelium-independent vascular reactivity were evaluated as well as the role of the reactive oxygen species (ROS). Superoxide and nitric oxide (NO) production were analyzed in situ using DHE and DAF-2 fluorescence, respectively. The expression of gp91phox and of the antioxidant enzymes were evaluated by western blotting in the thoracic aorta samples. MI promoted a significant increase in the contractile response and impaired endothelium-mediated relaxation. However, ET prevented the impairment in the vascular reactivity in MI animals. In addition, the protein expression of gp91phox and superoxide production increased and the NO production decreased in the OVX+MI(SED) group but not in the OVX+MI(ET) group. Therefore, ET improves vascular reactivity in MI ovariectomized rats by preventing the increase in the expression of gp91phox and the decrease in the antioxidant enzymes, resulting in a normal ROS and NO production. Thus, ET can be an effective therapeutic strategy for improving the MI-induced vascular alterations in estrogen deficiency condition. Public Library of Science 2019-04-24 /pmc/articles/PMC6481839/ /pubmed/31017961 http://dx.doi.org/10.1371/journal.pone.0215568 Text en © 2019 de Oliveira et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
de Oliveira, Suelen Guedes
Claudio, Erick Roberto Gonçalves
de Almeida, Simone Alves
Mengal, Vinicius
da Silva, Fabricio Bragança
Silva, Nyam Florêncio
Mauad, Helder
de Abreu, Glaucia Rodrigues
Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction
title Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction
title_full Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction
title_fullStr Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction
title_full_unstemmed Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction
title_short Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction
title_sort exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481839/
https://www.ncbi.nlm.nih.gov/pubmed/31017961
http://dx.doi.org/10.1371/journal.pone.0215568
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