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Propidium iodide staining underestimates viability of adherent bacterial cells
Combining membrane impermeable DNA-binding stain propidium iodide (PI) with membrane-permeable DNA-binding counterstains is a widely used approach for bacterial viability staining. In this paper we show that PI staining of adherent cells in biofilms may significantly underestimate bacterial viabilit...
Autores principales: | Rosenberg, Merilin, Azevedo, Nuno F., Ivask, Angela |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482146/ https://www.ncbi.nlm.nih.gov/pubmed/31019274 http://dx.doi.org/10.1038/s41598-019-42906-3 |
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