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sgRNA-shRNA Structure Mediated SNP Site Editing on Porcine IGF2 Gene by CRISPR/StCas9

The SNP within intron 3 of the porcine IGF2 gene (G3072A) plays an important role for muscle growth and fat deposition in pigs. In this study, the StCas9 derived from Streptococcus thermophilus together with the Drosha-mediated sgRNA-shRNA structure were combined to boost the G to A base editing on...

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Detalles Bibliográficos
Autores principales:	Sun, Yongsen, Yan, Nana, Mu, Lu, Sun, Bing, Deng, Jingrong, Fang, Yuanyuan, Shao, Simin, Yan, Qiang, Han, Furong, Zhang, Zhiying, Xu, Kun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2019
Materias:	Genetics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482158/ https://www.ncbi.nlm.nih.gov/pubmed/31057603 http://dx.doi.org/10.3389/fgene.2019.00347

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