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Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study

In mammals, glucosensing markers reside in brain areas known to play an important role in the control of food intake. The best characterized glucosensing mechanism is that dependent on glucokinase (GK) whose activation by increased levels of glucose leads in specific hypothalamic neurons to decrease...

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Autores principales: Otero-Rodiño, Cristina, Rocha, Ana, Sánchez, Elisa, Álvarez-Otero, Rosa, Soengas, José L., Cerdá-Reverter, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482260/
https://www.ncbi.nlm.nih.gov/pubmed/31057490
http://dx.doi.org/10.3389/fendo.2019.00254
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author Otero-Rodiño, Cristina
Rocha, Ana
Sánchez, Elisa
Álvarez-Otero, Rosa
Soengas, José L.
Cerdá-Reverter, José M.
author_facet Otero-Rodiño, Cristina
Rocha, Ana
Sánchez, Elisa
Álvarez-Otero, Rosa
Soengas, José L.
Cerdá-Reverter, José M.
author_sort Otero-Rodiño, Cristina
collection PubMed
description In mammals, glucosensing markers reside in brain areas known to play an important role in the control of food intake. The best characterized glucosensing mechanism is that dependent on glucokinase (GK) whose activation by increased levels of glucose leads in specific hypothalamic neurons to decreased or increased activity, ultimately leading to decreased food intake. In fish, evidence obtained in recent years suggested the presence of GK-like immunoreactive cells in different brain areas related to food intake control. However, it has not been established yet whether or not those neuronal populations having glucosensing capacity are the same that express the neuropeptides involved in the metabolic control of food intake. Therefore, we assessed through dual fluorescent in situ hybridization the possible expression of GK in the melanocortinergic neurons expressing proopiomelanocortin (POMC) or agouti-related protein (AGRP). POMC and AGRP expression localized exclusively in the rostral hypothalamus, in the ventral pole of the lateral tuberal nucleus, the homolog of the mammalian arcuate nucleus. Hypothalamic GK expression confined to the ependymal cells coating the ventral pole of the third ventricle but some expression level occurred in the AGRP neurons. GK expression seems to be absent in the hypothalamic POMC neurons. These results suggest that AGRP neurons might sense glucose directly through a mechanism involving GK. In contrast, POMC neurons would not directly respond to glucose through GK and would require presynaptic inputs to sense glucose. Ependymal cells could play a critical role relying glucose metabolic information to the central circuitry regulating food intake in fish, especially in POMC neurons.
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spelling pubmed-64822602019-05-03 Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study Otero-Rodiño, Cristina Rocha, Ana Sánchez, Elisa Álvarez-Otero, Rosa Soengas, José L. Cerdá-Reverter, José M. Front Endocrinol (Lausanne) Endocrinology In mammals, glucosensing markers reside in brain areas known to play an important role in the control of food intake. The best characterized glucosensing mechanism is that dependent on glucokinase (GK) whose activation by increased levels of glucose leads in specific hypothalamic neurons to decreased or increased activity, ultimately leading to decreased food intake. In fish, evidence obtained in recent years suggested the presence of GK-like immunoreactive cells in different brain areas related to food intake control. However, it has not been established yet whether or not those neuronal populations having glucosensing capacity are the same that express the neuropeptides involved in the metabolic control of food intake. Therefore, we assessed through dual fluorescent in situ hybridization the possible expression of GK in the melanocortinergic neurons expressing proopiomelanocortin (POMC) or agouti-related protein (AGRP). POMC and AGRP expression localized exclusively in the rostral hypothalamus, in the ventral pole of the lateral tuberal nucleus, the homolog of the mammalian arcuate nucleus. Hypothalamic GK expression confined to the ependymal cells coating the ventral pole of the third ventricle but some expression level occurred in the AGRP neurons. GK expression seems to be absent in the hypothalamic POMC neurons. These results suggest that AGRP neurons might sense glucose directly through a mechanism involving GK. In contrast, POMC neurons would not directly respond to glucose through GK and would require presynaptic inputs to sense glucose. Ependymal cells could play a critical role relying glucose metabolic information to the central circuitry regulating food intake in fish, especially in POMC neurons. Frontiers Media S.A. 2019-04-18 /pmc/articles/PMC6482260/ /pubmed/31057490 http://dx.doi.org/10.3389/fendo.2019.00254 Text en Copyright © 2019 Otero-Rodiño, Rocha, Sánchez, Álvarez-Otero, Soengas and Cerdá-Reverter. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Otero-Rodiño, Cristina
Rocha, Ana
Sánchez, Elisa
Álvarez-Otero, Rosa
Soengas, José L.
Cerdá-Reverter, José M.
Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study
title Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study
title_full Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study
title_fullStr Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study
title_full_unstemmed Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study
title_short Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study
title_sort sensing glucose in the central melanocortin circuits of rainbow trout: a morphological study
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482260/
https://www.ncbi.nlm.nih.gov/pubmed/31057490
http://dx.doi.org/10.3389/fendo.2019.00254
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