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Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study
In mammals, glucosensing markers reside in brain areas known to play an important role in the control of food intake. The best characterized glucosensing mechanism is that dependent on glucokinase (GK) whose activation by increased levels of glucose leads in specific hypothalamic neurons to decrease...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482260/ https://www.ncbi.nlm.nih.gov/pubmed/31057490 http://dx.doi.org/10.3389/fendo.2019.00254 |
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author | Otero-Rodiño, Cristina Rocha, Ana Sánchez, Elisa Álvarez-Otero, Rosa Soengas, José L. Cerdá-Reverter, José M. |
author_facet | Otero-Rodiño, Cristina Rocha, Ana Sánchez, Elisa Álvarez-Otero, Rosa Soengas, José L. Cerdá-Reverter, José M. |
author_sort | Otero-Rodiño, Cristina |
collection | PubMed |
description | In mammals, glucosensing markers reside in brain areas known to play an important role in the control of food intake. The best characterized glucosensing mechanism is that dependent on glucokinase (GK) whose activation by increased levels of glucose leads in specific hypothalamic neurons to decreased or increased activity, ultimately leading to decreased food intake. In fish, evidence obtained in recent years suggested the presence of GK-like immunoreactive cells in different brain areas related to food intake control. However, it has not been established yet whether or not those neuronal populations having glucosensing capacity are the same that express the neuropeptides involved in the metabolic control of food intake. Therefore, we assessed through dual fluorescent in situ hybridization the possible expression of GK in the melanocortinergic neurons expressing proopiomelanocortin (POMC) or agouti-related protein (AGRP). POMC and AGRP expression localized exclusively in the rostral hypothalamus, in the ventral pole of the lateral tuberal nucleus, the homolog of the mammalian arcuate nucleus. Hypothalamic GK expression confined to the ependymal cells coating the ventral pole of the third ventricle but some expression level occurred in the AGRP neurons. GK expression seems to be absent in the hypothalamic POMC neurons. These results suggest that AGRP neurons might sense glucose directly through a mechanism involving GK. In contrast, POMC neurons would not directly respond to glucose through GK and would require presynaptic inputs to sense glucose. Ependymal cells could play a critical role relying glucose metabolic information to the central circuitry regulating food intake in fish, especially in POMC neurons. |
format | Online Article Text |
id | pubmed-6482260 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64822602019-05-03 Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study Otero-Rodiño, Cristina Rocha, Ana Sánchez, Elisa Álvarez-Otero, Rosa Soengas, José L. Cerdá-Reverter, José M. Front Endocrinol (Lausanne) Endocrinology In mammals, glucosensing markers reside in brain areas known to play an important role in the control of food intake. The best characterized glucosensing mechanism is that dependent on glucokinase (GK) whose activation by increased levels of glucose leads in specific hypothalamic neurons to decreased or increased activity, ultimately leading to decreased food intake. In fish, evidence obtained in recent years suggested the presence of GK-like immunoreactive cells in different brain areas related to food intake control. However, it has not been established yet whether or not those neuronal populations having glucosensing capacity are the same that express the neuropeptides involved in the metabolic control of food intake. Therefore, we assessed through dual fluorescent in situ hybridization the possible expression of GK in the melanocortinergic neurons expressing proopiomelanocortin (POMC) or agouti-related protein (AGRP). POMC and AGRP expression localized exclusively in the rostral hypothalamus, in the ventral pole of the lateral tuberal nucleus, the homolog of the mammalian arcuate nucleus. Hypothalamic GK expression confined to the ependymal cells coating the ventral pole of the third ventricle but some expression level occurred in the AGRP neurons. GK expression seems to be absent in the hypothalamic POMC neurons. These results suggest that AGRP neurons might sense glucose directly through a mechanism involving GK. In contrast, POMC neurons would not directly respond to glucose through GK and would require presynaptic inputs to sense glucose. Ependymal cells could play a critical role relying glucose metabolic information to the central circuitry regulating food intake in fish, especially in POMC neurons. Frontiers Media S.A. 2019-04-18 /pmc/articles/PMC6482260/ /pubmed/31057490 http://dx.doi.org/10.3389/fendo.2019.00254 Text en Copyright © 2019 Otero-Rodiño, Rocha, Sánchez, Álvarez-Otero, Soengas and Cerdá-Reverter. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Otero-Rodiño, Cristina Rocha, Ana Sánchez, Elisa Álvarez-Otero, Rosa Soengas, José L. Cerdá-Reverter, José M. Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study |
title | Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study |
title_full | Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study |
title_fullStr | Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study |
title_full_unstemmed | Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study |
title_short | Sensing Glucose in the Central Melanocortin Circuits of Rainbow Trout: A Morphological Study |
title_sort | sensing glucose in the central melanocortin circuits of rainbow trout: a morphological study |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482260/ https://www.ncbi.nlm.nih.gov/pubmed/31057490 http://dx.doi.org/10.3389/fendo.2019.00254 |
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