First fungemia case due to environmental yeast Wickerhamomyces myanmarensis: detection by multiplex qPCR and antifungal susceptibility

AIM: Presenting the first clinical case of Wickerhamomyces myanmarensis. PATIENTS & METHODS: Yeast cells were isolated from blood and central venous catheter of a 5.5-year-old male subject. API 20C AUX, MALDI-TOF MS, ITS and LSU rDNA sequencing, and our qPCR assay were used for identification an...

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Detalles Bibliográficos
Autores principales: Arastehfar, Amir, Bakhtiari, Mina, Daneshnia, Farnaz, Fang, Wenjie, Sadati, Sara Khanjari, Al-Hatmi, Abdullah MS, Groenewald, Marizeth, Sharifi-Mehr, Hamid, Liao, Wanqing, Pan, Weihua, Zomorodian, Kamiar, Hagen, Ferry, Boekhout, Teun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Medicine Ltd 2019
Materias:
Case Series
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482385/
https://www.ncbi.nlm.nih.gov/pubmed/30859860
http://dx.doi.org/10.2217/fmb-2018-0253
Descripción
Sumario:AIM: Presenting the first clinical case of Wickerhamomyces myanmarensis. PATIENTS & METHODS: Yeast cells were isolated from blood and central venous catheter of a 5.5-year-old male subject. API 20C AUX, MALDI-TOF MS, ITS and LSU rDNA sequencing, and our qPCR assay were used for identification and the MIC values were determined by CLSI M27-A3. RESULTS: ITS and LSU rDNA sequencing identified both isolates as W. myanmarensis, while API 20C AUX and MALDI-TOF MS did not identify them correctly. Our qPCR specifically distinguished W. myanmarensis from W. anomalus. Isolate obtained from blood showed a higher MIC value for fluconazole, voriconazole and posaconazole. CONCLUSION: Utilization of reliable identification tools might reveal the genuine spectrum of opportunistic yeast species.

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