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Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride
BACKGROUND: Long-term in vitro culture of blood stage Plasmodium parasites invariably leads to asynchronous parasite development. The most often used technique to synchronize Plasmodium falciparum culture is sorbitol treatment, which differentially induces osmotic lysis of trophozoite- and schizont-...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482532/ https://www.ncbi.nlm.nih.gov/pubmed/31023359 http://dx.doi.org/10.1186/s12936-019-2783-1 |
_version_ | 1783413901110542336 |
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author | Ngernna, Sutharinee Chim-ong, Anongruk Roobsoong, Wanlapa Sattabongkot, Jetsumon Cui, Liwang Nguitragool, Wang |
author_facet | Ngernna, Sutharinee Chim-ong, Anongruk Roobsoong, Wanlapa Sattabongkot, Jetsumon Cui, Liwang Nguitragool, Wang |
author_sort | Ngernna, Sutharinee |
collection | PubMed |
description | BACKGROUND: Long-term in vitro culture of blood stage Plasmodium parasites invariably leads to asynchronous parasite development. The most often used technique to synchronize Plasmodium falciparum culture is sorbitol treatment, which differentially induces osmotic lysis of trophozoite- and schizont-infected red blood cells due to presence of the new permeation pathways in the membranes of these cells. However, sorbitol treatment does not work well when used to synchronize the culture-adapted Plasmodium knowlesi A1-H.1 line. METHODS: A number of common solutes were tested in lieu of sorbitol for synchronization of P. knowlesi A1-H.1 ring stage. RESULTS: Guanidine hydrochloride was found to selectively lyse trophozoite- and schizont-infected red blood cells, yielding highly synchronous and viable rings. CONCLUSIONS: A method for synchronization of P. knowlesi in human red blood cells was developed. Requiring only common laboratory reagents, this method is simple and should be applicable to most laboratory settings. |
format | Online Article Text |
id | pubmed-6482532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-64825322019-05-02 Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride Ngernna, Sutharinee Chim-ong, Anongruk Roobsoong, Wanlapa Sattabongkot, Jetsumon Cui, Liwang Nguitragool, Wang Malar J Methodology BACKGROUND: Long-term in vitro culture of blood stage Plasmodium parasites invariably leads to asynchronous parasite development. The most often used technique to synchronize Plasmodium falciparum culture is sorbitol treatment, which differentially induces osmotic lysis of trophozoite- and schizont-infected red blood cells due to presence of the new permeation pathways in the membranes of these cells. However, sorbitol treatment does not work well when used to synchronize the culture-adapted Plasmodium knowlesi A1-H.1 line. METHODS: A number of common solutes were tested in lieu of sorbitol for synchronization of P. knowlesi A1-H.1 ring stage. RESULTS: Guanidine hydrochloride was found to selectively lyse trophozoite- and schizont-infected red blood cells, yielding highly synchronous and viable rings. CONCLUSIONS: A method for synchronization of P. knowlesi in human red blood cells was developed. Requiring only common laboratory reagents, this method is simple and should be applicable to most laboratory settings. BioMed Central 2019-04-25 /pmc/articles/PMC6482532/ /pubmed/31023359 http://dx.doi.org/10.1186/s12936-019-2783-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Methodology Ngernna, Sutharinee Chim-ong, Anongruk Roobsoong, Wanlapa Sattabongkot, Jetsumon Cui, Liwang Nguitragool, Wang Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride |
title | Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride |
title_full | Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride |
title_fullStr | Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride |
title_full_unstemmed | Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride |
title_short | Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride |
title_sort | efficient synchronization of plasmodium knowlesi in vitro cultures using guanidine hydrochloride |
topic | Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482532/ https://www.ncbi.nlm.nih.gov/pubmed/31023359 http://dx.doi.org/10.1186/s12936-019-2783-1 |
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