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Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride

BACKGROUND: Long-term in vitro culture of blood stage Plasmodium parasites invariably leads to asynchronous parasite development. The most often used technique to synchronize Plasmodium falciparum culture is sorbitol treatment, which differentially induces osmotic lysis of trophozoite- and schizont-...

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Autores principales: Ngernna, Sutharinee, Chim-ong, Anongruk, Roobsoong, Wanlapa, Sattabongkot, Jetsumon, Cui, Liwang, Nguitragool, Wang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482532/
https://www.ncbi.nlm.nih.gov/pubmed/31023359
http://dx.doi.org/10.1186/s12936-019-2783-1
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author Ngernna, Sutharinee
Chim-ong, Anongruk
Roobsoong, Wanlapa
Sattabongkot, Jetsumon
Cui, Liwang
Nguitragool, Wang
author_facet Ngernna, Sutharinee
Chim-ong, Anongruk
Roobsoong, Wanlapa
Sattabongkot, Jetsumon
Cui, Liwang
Nguitragool, Wang
author_sort Ngernna, Sutharinee
collection PubMed
description BACKGROUND: Long-term in vitro culture of blood stage Plasmodium parasites invariably leads to asynchronous parasite development. The most often used technique to synchronize Plasmodium falciparum culture is sorbitol treatment, which differentially induces osmotic lysis of trophozoite- and schizont-infected red blood cells due to presence of the new permeation pathways in the membranes of these cells. However, sorbitol treatment does not work well when used to synchronize the culture-adapted Plasmodium knowlesi A1-H.1 line. METHODS: A number of common solutes were tested in lieu of sorbitol for synchronization of P. knowlesi A1-H.1 ring stage. RESULTS: Guanidine hydrochloride was found to selectively lyse trophozoite- and schizont-infected red blood cells, yielding highly synchronous and viable rings. CONCLUSIONS: A method for synchronization of P. knowlesi in human red blood cells was developed. Requiring only common laboratory reagents, this method is simple and should be applicable to most laboratory settings.
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spelling pubmed-64825322019-05-02 Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride Ngernna, Sutharinee Chim-ong, Anongruk Roobsoong, Wanlapa Sattabongkot, Jetsumon Cui, Liwang Nguitragool, Wang Malar J Methodology BACKGROUND: Long-term in vitro culture of blood stage Plasmodium parasites invariably leads to asynchronous parasite development. The most often used technique to synchronize Plasmodium falciparum culture is sorbitol treatment, which differentially induces osmotic lysis of trophozoite- and schizont-infected red blood cells due to presence of the new permeation pathways in the membranes of these cells. However, sorbitol treatment does not work well when used to synchronize the culture-adapted Plasmodium knowlesi A1-H.1 line. METHODS: A number of common solutes were tested in lieu of sorbitol for synchronization of P. knowlesi A1-H.1 ring stage. RESULTS: Guanidine hydrochloride was found to selectively lyse trophozoite- and schizont-infected red blood cells, yielding highly synchronous and viable rings. CONCLUSIONS: A method for synchronization of P. knowlesi in human red blood cells was developed. Requiring only common laboratory reagents, this method is simple and should be applicable to most laboratory settings. BioMed Central 2019-04-25 /pmc/articles/PMC6482532/ /pubmed/31023359 http://dx.doi.org/10.1186/s12936-019-2783-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology
Ngernna, Sutharinee
Chim-ong, Anongruk
Roobsoong, Wanlapa
Sattabongkot, Jetsumon
Cui, Liwang
Nguitragool, Wang
Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride
title Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride
title_full Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride
title_fullStr Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride
title_full_unstemmed Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride
title_short Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride
title_sort efficient synchronization of plasmodium knowlesi in vitro cultures using guanidine hydrochloride
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6482532/
https://www.ncbi.nlm.nih.gov/pubmed/31023359
http://dx.doi.org/10.1186/s12936-019-2783-1
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